Abstract
The oxidative stress and antioxidant systems in soybean leaves and roots infected with plant pathogen Aspergillus niger were studied following treatment with different concentrations of cholic acid. Several oxidative stress parameters were analyzed: production of superoxide (O2 ·−) and hydroxyl radicals (·OH), lipid peroxidation (LP), and superoxide dismutase (SOD; EC 1.15.1.1) activity, as well as the content of reduced glutathione (GSH). Results showed that inoculation with A. niger led to the increase of O2 ·− production and GSH quantities in leaves and ·OH in roots. The highest activity of SOD occured in infected plants treated with cholic acid in concentrations of 40 and 60 mg L−1 which ultimately led to a decrease in O2 ·− production. Inoculation with Aspergillus in combination with elevated cholic acid concentrations also increased ·OH production which is correlated with increased LP. These results may support the idea of using cholic acid as an elicitor to trigger hypersensitive response in plant cells. Use of cholic acid may also actively contribute to soybean plants defense response against pathogen attack.
[1] Boue S.M., Carter C.H., Ehrlich K.C., Cleveland T.E., Induction of the soybean phytoalexins coumesttrol and glyceollin by Aspergillus, J. Agric. Food Chem., 2000, 48, 2167–2172 http://dx.doi.org/10.1021/jf991280910.1021/jf9912809Search in Google Scholar
[2] Govrin E.M., Levine A., The hypersensitive response facilitates plant infection by the necrotrophic pathogen Botrytis cinerea, Curr. Biol., 2000, 10, 751–757 http://dx.doi.org/10.1016/S0960-9822(00)00560-110.1016/S0960-9822(00)00560-1Search in Google Scholar
[3] Pontier D., Balague C., Roby D., The hypersensitive response. A programmed cell death associated with plant resistance, C.R. Acad. Sci Paris, Sciences de la vie, 1988, 321, 721–734 10.1016/S0764-4469(98)80013-9Search in Google Scholar
[4] Greenberg J.T., Programmed cell death in plant pathogen interactions, Annu. Rev. Plant Physiol. Plant Mol. Biol., 1997, 48, 525–545 http://dx.doi.org/10.1146/annurev.arplant.48.1.52510.1146/annurev.arplant.48.1.525Search in Google Scholar PubMed
[5] Scandalios J.G., Oxygen stress and superoxide dismutases, Plant Physiol., 1993, 101, 7–12 10.1104/pp.101.1.7Search in Google Scholar PubMed PubMed Central
[6] Blokhina O., Virolainen E., Fagerstedt K.V., Antioxidants, oxidative damage and oxygen deprivation stress: a Review, Ann. Bot., 2003, 91, 179–194 http://dx.doi.org/10.1093/aob/mcf11810.1093/aob/mcf118Search in Google Scholar PubMed PubMed Central
[7] Malenčić Dj., Vasić D., Popović M., Dević D., Antioxidant systems in sunflower as affected by oxalic acid, Biol. Plant., 2004, 48, 243–247 http://dx.doi.org/10.1023/B:BIOP.0000033451.96311.1810.1023/B:BIOP.0000033451.96311.18Search in Google Scholar
[8] Kwon S.I., Anderson A.J., Differential production of superoxide dismutase and catalase isozymes during infection of wheat by a Fusarium proliferatum-like fungal isolate, Physiol. Mol. Plant Pathol., 2001, 58, 73–81 http://dx.doi.org/10.1006/pmpp.2000.031310.1006/pmpp.2000.0313Search in Google Scholar
[9] Malenčić Dj., Kiprovski B., Popović M., Prvulović D., Miladinović J., Djordjević V., Changes in antioxidant system in soybean as affected by Sclerotinia sclerotiorum (Lib.) de Bary, Plant Physiol. Biochem., 2010, 48, 903–908 http://dx.doi.org/10.1016/j.plaphy.2010.08.00310.1016/j.plaphy.2010.08.003Search in Google Scholar PubMed
[10] Mandal S., Mitra A., Mallick N., Biochemical characterization of oxidative burst during interaction between Solanum lycopersicum and Fusarium oxysporum f. sp. lycopersici, Physiol. Mol. Plant Pathol., 2008, 72, 56–61 http://dx.doi.org/10.1016/j.pmpp.2008.04.00210.1016/j.pmpp.2008.04.002Search in Google Scholar
[11] Koga J., Kubota H., Gomi S., Umemura K., Ohnishi M., Kono T., Cholic acid, a bile acid elicitor of hypersensitive cell death, pathogenesis-related protein synthesis, and phytoalexin accumulation in rice, Plant Physiol., 2006, 140, 1475–1483 http://dx.doi.org/10.1104/pp.105.07033410.1104/pp.105.070334Search in Google Scholar
[12] Shimizu T., Jikumaru Y., Okada A., Okada K., Koga J., Umemura K., et al., Effects of a bile acid elicitor, cholic acid, on the biosynthesis of diterpenoid phytoalexins in suspension-cultured rice cells, Phytochemistry, 2008, 69, 973–81 http://dx.doi.org/10.1016/j.phytochem.2007.10.00510.1016/j.phytochem.2007.10.005Search in Google Scholar
[13] Blee K.A., Jupe S.C., Richard G., Zimmerlin A., Davies D.R., Bolwell G.P., Molecular identification and expression of the peroxidase responsible for the oxidative burst in French bean (Phaseolus vulgaris L.) and related members of the gene family, Plant Mol. Biol., 2001, 47, 607–620 http://dx.doi.org/10.1023/A:101230732478210.1023/A:1012307324782Search in Google Scholar
[14] Kawano T., Pinontoan R., Uozumi N., Morimitsu Y., Miyake C., Asada K., et al., Phenylethylamine-induced generation of reactive oxygen species and ascorbate free radicals in tobacco suspension culture: mechanism for oxidative burst mediating Ca2+ influx, Plant Cell Physiol., 2000, 41, 1259–1266 http://dx.doi.org/10.1093/pcp/pcd05310.1093/pcp/pcd053Search in Google Scholar
[15] Sokol R.J., Winklhofer-Roob B.M., Devereaux M.W., McKim Jr. J.M., Generation of hydroperoxides in isolated rat hepatocytes and hepatic mitochondria exposed to hydrophobic bile acids, Gastroenterology, 1995, 109, 1249–1256 http://dx.doi.org/10.1016/0016-5085(95)90585-510.1016/0016-5085(95)90585-5Search in Google Scholar
[16] Palmeira C.M., Rolo A.P., Mitochondrially-mediated toxicity of bile acids, Toxicology, 2004, 203, 1–15 http://dx.doi.org/10.1016/j.tox.2004.06.00110.1016/j.tox.2004.06.001Search in Google Scholar
[17] Kevrešan S.E., Malenčić Dj.R., Popović M.T., Kuhajda K.N., Kandrač J.E., The effect of cholic acid treatment on the oxidative status of soybean plants, J. Serb. Chem. Soc., 2009, 74, 857–865 http://dx.doi.org/10.2298/JSC0909857K10.2298/JSC0909857KSearch in Google Scholar
[18] Misra H.P., Fridovich I., The role of superoxide anion in the autooxidation of epinephrine and a simple measurement for superoxide dismutase, J. Biol. Chem., 1972, 247, 3170–3175 10.1016/S0021-9258(19)45228-9Search in Google Scholar
[19] Cheesman K.H., Beavis A., Esterbaurer H., Hydroxyl-radical-induced iron-catalysed degradation of 2-deoxyribose, Biochem. J., 1988, 252, 649–653 10.1042/bj2520649Search in Google Scholar
[20] Placer Z.A., Cushman L.L., Johnson B.C., Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems, Anal. Biochem., 1966, 16, 359–364 http://dx.doi.org/10.1016/0003-2697(66)90167-910.1016/0003-2697(66)90167-9Search in Google Scholar
[21] Gidrol H., Sergihini H., Nobhani B., Mocquot B., Mazilak P., Biochemical changes induced by accelerated agent of sunflower seeds. Lipid peroxidation and membrane damage, Physiol. Plant., 1987, 76, 591–598 http://dx.doi.org/10.1111/j.1399-3054.1989.tb05484.x10.1111/j.1399-3054.1989.tb05484.xSearch in Google Scholar
[22] Sedlak J., Lindsay H., Estimation of total protein bound and non protein sulphydryl groups in tissue with Ellman’s reagent, Anal Biochem, 1968, 25, 192–205 http://dx.doi.org/10.1016/0003-2697(68)90092-410.1016/0003-2697(68)90092-4Search in Google Scholar
[23] Doke N., Generation of superoxide anion by potato tuber protoplasts during the hypersensitive response to hyphal wall components of Phytophthora infestans and specific inhibition of the reaction by suppressors of hypersensitivity, Physiol. Plant Pathol., 1983, 23, 359–367 10.1016/0048-4059(83)90020-6Search in Google Scholar
[24] Lamb C., Dixon R.A., The oxidative burst in plant disease resistance, Annu. Rev. Plant Physiol. Plant Mol. Biol., 1997, 48, 251–275 http://dx.doi.org/10.1146/annurev.arplant.48.1.25110.1146/annurev.arplant.48.1.251Search in Google Scholar PubMed
[25] Delledonne M., Zeier J., Marocco A., Lamb C., Signal interactions between nitric oxide and reactive oxygen intermediates in the plant hypersensitive disease resistance response, Proceed. Nat. Acad. Sci. USA, 2001, 98, 13454–13459 http://dx.doi.org/10.1073/pnas.23117829810.1073/pnas.231178298Search in Google Scholar PubMed PubMed Central
[26] Delledonne M., Xia Y., Dixon R.A., Lamb C., Nitric oxide functions as a signal in plant disease resistance, Nature (London), 1988, 394, 585–588 10.1038/29087Search in Google Scholar PubMed
[27] Gupta A.S., Webb R.P., Holaday A.S., Allen R.D., Overexpression of superoxide dismutase protects plants from oxidative stress, Plant Physiol., 1993, 103, 1067–1073 10.1104/pp.103.4.1067Search in Google Scholar PubMed PubMed Central
[28] Lukivskaya O., Zavodnik L., Knas M., Buko V., Antioxidant mechanism of hepatoprotection by ursodeoxycholic acid in experimental alcoholic steatohepatitis, Adv. Med. Sci., 2006, 51, 54–59 Search in Google Scholar
[29] Arias I.M., Jakoby W.B., Glutathione: Metabolism and Function, Raven Press, New York, 1976 Search in Google Scholar
[30] Palma J.M., Gomez M., Yanez J., Del Rio L.A., Increased levels of peroxisomal active oxygenrelated enzymes in copper-tolerant pea plants, Plant Physiol., 1987, 85, 570–574 http://dx.doi.org/10.1104/pp.85.2.57010.1104/pp.85.2.570Search in Google Scholar PubMed PubMed Central
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