Abstract
DNA methylation pattern could be considered a biomarker to be exploited for the study and management of several human diseases. In this chapter, detailed protocols are provided for two experimental approaches used for quantitative methylation analysis of bisulfite converted DNA: methylation-specific PCR (MSP) and pyrosequencing.
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D’Addario, C., Pucci, M. (2023). DNA Methylation Analysis of Cnr1 Gene Promoter. In: Maccarrone, M. (eds) Endocannabinoid Signaling. Methods in Molecular Biology, vol 2576. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2728-0_31
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DOI: https://doi.org/10.1007/978-1-0716-2728-0_31
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