Journal of Biomedical Engineering Research (대한의용생체공학회:의공학회지)

The Korean Society of Medical and Biological Engineering (대한의용생체공학회)
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Surface Immobilization of $(1{\to}3)(1{\to}6)-{\beta}-glucan$ onto Biodegradable Polymer for Tissue Regeneration

조직 재생을 위한 Poly (D, L-lactide-co-glycolide) 표면에 $(1{\to}3)(1{\to}6)-{\beta}-glucan$ 고정에 대한 세포 점착 및 성장 효과

  • Lee, S.G. (Dept. of Biomedical Engineering, Inje Univ.) ;
  • Lee, J.B. (Dept. of Biomedical Engineering, Inje Univ.) ;
  • Yu, S.M. (Dept. of Biomedical Engineering, Inje Univ.) ;
  • Park, J.C. (Dept. Medical Eng., Collage of Med., Yonsei Univ.) ;
  • Choi, J.B. (Dept. Mechanical Systems Eng., Hansung Univ.) ;
  • Kim, J.K. (Dept. of Biomedical Engineering, Inje Univ.)
  • 이상길 (인제대학교 의용공학과) ;
  • 이정복 (인제대학교 의용공학과) ;
  • 유성미 (인제대학교 의용공학과) ;
  • 박종철 (연세의과대학 의학공학교실) ;
  • 최재봉 (한성대학 기계시스템공학과) ;
  • 김정구 (인제대학교 의용공학과)
  • Published : 2006.10.31
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Abstract

We examined the effects of ${\beta}$-glucan-reinforced PLGA film and scaffold on HDFs (human dermal fibroblast) attachment and proliferation. The PLGA films were prepared by simple solvent-casting method. The prepared films were grafted with $(1{\to}3)(1{\to}6)-{\beta}-glucan$ in various ratios after plasma treatment on surface. The surface of the film was characterized by contact angle measurement, scanning electron microscope (SEM), and Fourier-transform infrared spectrophotometer (FT-IR). The amount of $(1{\to}3)(1{\to}6)-{\beta}-glucan$ in the prepared film was indirectly determined by phenol-sulfuric acid method. The HDFs (Human dermal fibroblasts) were used to evaluate the cell attachment and proliferation on PLGA specimens before and after plasma/${\beta}-glucan$ treatment. The result showed that the plasma treated groups exhibited more mont of ${\beta}-glucan$ might be grafted than the non plasma treated groups. Cell attachment was significantly enhanced in the plasma/${\beta}-glucan$ grafted group after 4 hours incubation (p<0.05) due to the improved hydrophilicity and cytoactivity effect of the ${\beta}-glucan$. The cell proliferation of plasma/${\beta}-glucan$ (2mg/ml) grafted group was the highest rate among the groups (p<0.05).

Keywords

References

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