The Antioxidative, Antimicrobial Activity and HPLC Analysis of Ornithogalum pyrenaicum

Biological Activity of Ornithogalum pyrenaicum

Authors

DOI:

https://doi.org/10.62482/pmj.2

Keywords:

Antimicrobial activity, antioxidant activity, Ornithogalum pyrenaicum, RP-HPLC, total phenolic content

Abstract

Introduction: The aim of this study was to specify the antioxidant, antimicrobial activity and phenolic contents of Ornithogalum pyrenaicum.

Methods: The antioxidant capacity of O. pyrenaicum was determined by 2,2-diphenylpicrylhydrazyl (DPPH) method and ferric reduction antioxidant power by FRAP method. The total phenolic content (TPC) was of the samples was determined using spectrophotometric method. The phenolic contents the samples were analyzed by reverse phase-high performance liquid chromatography (RP-HPLC). Antimicrobial activity was researched on 9 microorganism by agar diffusion method.

Results: As a result of the study, high phenolic contents and strong antioxidant capacitiy were observed. Phenolic compounds were detected as p-coumaric acid and benzoic acid.  Additionally, it was determined that O. pyrenacium had considerable antimicrobial activity on Yersinia pseudotuberculosis, Staphylococcus aureus and moderate activity on Pseudomonas aeruginosa and Mycobacterium smegmatis.

Conclusion: In conclusion, O. pyrenaicum extract could be evaluated in the pharmaceutical and cosmetic fields due to their antioxidant and antimicrobial potential and phenolic compounds.

References

Yalçın CÖ, Yılmaz Sarıaltın S, Çiçek Polat D. Quantification of phenolic and flavonoid contents and some biological activities of Ornithogalum sigmoideum Freyn & Sint. J. Res. Pharm. 2020; 24(4): 487-496. Available from: https://doi.org/10.35333/jrp.2020.197

Zengin G, Uysal S, Ceylan R, Aktumsek A. Phenolic constituent, antioxidative and tyrosinase inhibitory activity of Ornithogalum narbonense L. from Turkey: a phytochemical study. Ind. Crops Prod. 2015; 70: 1-6. Available from: https://doi.org/10.1016/j.indcrop.2015.03.012

Pfosser M, Speta F. Phylogenetics of Hyacinthaceae based on plastid DNA sequences. Ann. Missouri Bot. Gard. 1999; 86 (4): 852-857. Available from: https://doi.org/10.2307/2666172

Goldblatt P, Manning JC. A review of chromosome cytology in Hyacinthaceae subfamily Ornithogaloideae (Albuca, Dipcadi, Ornithogalum and Pseudogaltonia) in sub- Saharan Africa. S. Afr. J. Bot. 2011; 77 (3): 581-591. Available from: https://doi.org/10.1016/j.sajb.2011.03.011

Martinez-Azorin M, Crespo MB, Juan A, Fay MF. Molecular phylogenetics of subfamily Ornithogaloideae (Hyacinthaceae) b new taxonomic arrangement. Ann. Bot. 2011; 107 (1): 1-37. Available from: https://doi.org/10.1093/aob/mcq207

Melzer D. New drug treatment for Alzheimer’s diseases: Lessons for healthcare policy. BMJ [Internet]. 1998 Mar 7; 316(7133): 762-764. Available from: https://doi.org/10.1136/bmj.316.7133.762

Bacanlı M, Taner G, Başaran AA, Başaran N. Bitkisel Kaynaklı Fenolik Yapıdaki Bileşikler ve Sağlığa Yararlı Etkileri. Turkiye Klinikleri J. Pharm. Sci. 2015; 4 (1): 9-16. doi: 10.5336/pharmsci.2014-42688

Kambizi L, Afolayan AJ. An ethnobotanical study of plants used for the treatment of sexually transmitted diseases (njovhera) in Guruve district, Zimbabwe. J. Ethnopharmacol. 2001 Sep; 77(1): 5-9. Available from: https://doi.org/10.1016/S0378-8741(01)00251-3

Beermann C, Gruschwitz N, Walkowski K, Gopel A. Growth modulating properties of polyphenolic apple pomace extract on food associated microorganisms. J. Microbiol. Biotechnol. Food Sci. 2013; 3(2): 176-181. Available from: https://www.researchgate.net/publication/327437645

Brantner A, Maleš Ž, Pepeljnjak S, Antolić A. Antimicrobial activity of Paliurus spina-christi Mill.(Christs thorn). J. Ethnopharmacol. 1996 Jun; 52 (2): 119-122. Available from: https://doi.org/10.1016/0378-8741(96)01408-0

Pinho E, Soares G, Henriques M. Evaluation of antibacterial activity of caffeic acid encapsulated by β-cyclodextrins. J. Microencapsul. 2015 Oct 7; 32(8): 804-810. Available from: https://doi.org/10.3109/02652048.2015.1094531

Kırıcı M, Ispir U, Seker E. Influence of Caffeic Acid on the Inhibition of Yersinia ruckeri.

J. Appl. Biol. Sci. 2016; 10 (1): 47-49.

Cowan MM. Plant products as antimicrobial agents. Clin. Microbiol. Rev. 1999 Oct 1; 12 (4): 564-582. Available from: https://doi.org/10.1128/cmr.12.4.564

Singleton VL, Rossi JA Jr. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic. 1965 Jan 1; 16 (3): 144-158.

Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal. Biochem. 1996 Jul 15; 239 (1): 70-76. Available from: https://doi.org/10.1006/abio.1996.0292

Molyneux P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. Sci. Technol. 2004 Mar-Apr; 26 (2): 211-219. Available from: https://www.researchgate.net/publication/237620105

Perez C, Pauli M, Bazerque P. An antibiotic assay by the well agar method. Acta Biol. Med. Exp. 1990 Jan; 15: 113-115. Available from: https://www.researchgate.net/publication/303960600

Aliyazicioglu R, Demir S, Badem M, Sener SO, Korkmaz N et al. Antioxidant, antigenotoxic, antimicrobial activities and phytochemical analysis of Dianthus carmelitarum. Rec. Nat. Prod. 2017 Jan; 11 (3): 270-284. Available from: https://www.researchgate.net/publication/317742636

Macdougall P. Fruitful synthesis of science and fiction. Nature. 2002 Jan 3; 415(6867): 13-14. Available from: https://doi.org/10.1038/415013d

Scalbert A, Manach C, Morand C, Remesy C, Jimenez L. Dietary polyphenols and the prevention of diseases. Crit. Rev. Food Sci. Nutr. 2005; 45(4): 287-306. Available from: https://doi.org/10.1080/1040869059096

Kolaç T, Gürbüz P, Yetiş G. Doğal ürünlerin fenolik içeriği ve antioksidan özellikleri. İ. Ü. Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi, 2017 May 26; 5(1): 26-42. Available from: https://dergipark.org.tr/tr/pub/inonusaglik/issue/29401/410872

Malkoç M, Çakır H, Kara Y, Can Z, Kolaylı S. Phenolic composition and antioxidant properties of Anzer honey from black sea region of Turkey. U. Bee J. 2019 Sept 6; 19 (2): 143-151. Available from: https://doi.org/10.31467/uluaricilik.602906

Kong CS, Jeong CH, Choi JS, Kim KJ, Jeong JW. Antiangiogenic effects of p‐coumaric acid in human endothelial cells. Phytother. Res. 2013 Mar; 27 (3): 317-323. Available from: https://doi.org/10.1002/ptr.4718

Chen R, Li Y, Dong H, Liu Z, Li S et al. Optimization of ultrasonic extraction process of polysaccharides from Ornithogalum caudatum Ait and evaluation of its biological activities. Ultrason. Sonochem. 2012 Nov; 19 (6): 1160-1168. Available from: https://doi.org/10.1016/j.ultsonch.2012.03.008

Ebrahimzadeh MA, Nabavl SM, Nabavl SF, Eslami B. Antioxidant activity of the bulb and aerial parts of Ornithogalum sintenisii L (Liliaceae) at flowering stage. Trop. J. Pharm. Res. 2010 Apr; 9 (2): 141-148. Available from: Available from: http://www.tjpr.org

Ouerghemmi I, Rebey IB, Rahali FZ, Bourgou S, Pistelli L, Ksouri R, Marzouk B, Tounsi MS. Antioxidant and antimicrobial phenolic compounds from extracts of cultivated and wild-grown Tunisian Ruta chalepensis. J. Food Drug Anal. 2017 Apr; 25 (2): 350-359. Available from: http://dx.doi.org/10.1016/j.jfda.2016.04.001

Tsugo K, Nakamura SI, Yamanaka H, Une Y. A study on the efficacy of the recombinant Yersinia adhesin A vaccine against yersiniosis in the early phase. J. Vet. Med. Sci. 2017 Mar 20; 79(5): 855-863. doi: 10.1292/jvms.16-0528

Şen Akoğlu A, Halkman AK. Çiğ sütte Pseudomonas aeruginosa sayılması için yöntem modifikasyonları üzerine çalışmalar. Orlab On-Line Mikrobiyoloji Dergisi. 2006; 4 (4): 21. Available from: www.mikrobiyoloji.org/pdf/702060402.pdf

Best CA, Best TJ. Mycobacterium smegmatis infection of the hand. Hand (NY). 2009 Jun; 4 (2): 165-166. Available from: https://doi.org/10.1007/s11552-008-9147-6

Downloads

Published

2024-02-29

How to Cite

Aliyazicioglu, R., Kanbolat, Şeyda, Badem, M., Sener, S. O., Korkmaz, N., Ozgen, U., & Alpay Karaoglu, S. (2024). The Antioxidative, Antimicrobial Activity and HPLC Analysis of Ornithogalum pyrenaicum: Biological Activity of Ornithogalum pyrenaicum. Pharmedicine Journal, 1(1), 20–26. https://doi.org/10.62482/pmj.2

Issue

Section

Articles