Simultaneous off-axis multiplexed holography and regular fluorescence microscopy of biological cells
Posted on 2018-05-22 - 20:33
We present a new technique for obtaining simultaneous multimodal quantitative phase and fluorescence microscopy of biological cells, providing both quantitative phase imaging and molecular specificity using a single camera. Our system is based on an interferometric multiplexing module, externally positioned at the exit of an optical microscope. In contrast to previous approaches, the presented technique allows conventional fluorescence imaging, rather than interferometric off-axis fluorescence imaging. We demonstrate the presented technique for imaging fluorescent beads and live biological cells.
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Nygate, Yoav; Singh, Gyanendra; Barnea, Itay; Shaked, Natan (2018). Simultaneous off-axis multiplexed holography and regular fluorescence microscopy of biological cells. Optica Publishing Group. Collection. https://doi.org/10.6084/m9.figshare.c.4041035.v1
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AUTHORS (4)
YN
Yoav Nygate
GS
Gyanendra Singh
IB
Itay Barnea
NS
Natan Shaked