Darkfield orthogonal polarized spectral imaging for studying endovascular laser-tissue interactions in vivo-a preliminary study

Due to the limited number of suitable intravital microscopy techniques, relatively little is known about the opto-thermal (endo)vascular responses to selective photothermolysis, used as a default treatment modality for superficial vascular anomalies such as port wine stains, telangiectasias, and hemangiomas. In this preliminary study we present a novel microscopy technique for studying (endo)vascular laser-tissue interactions in vivo, in which conventional orthogonal polarized spectral (OPS) imaging is combined with darkfield (DF) illumination. DFOPS imaging of rat mesenteric vasculature irradiated at increasing powers revealed the following (tissular) responses: formation of translucent aggregates, retrograde flow, gradual and immediate hemostasis, reinstatement of flow, vessel disappearance, and perivascular collagen damage. DFOPS imaging therefore constitutes a useful tool for examining (endo)vascular events following selective photothermolysis.