Allen, Richard (2021). CIL:9018, Coleps hirtus, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0W37VB1

A high resolution view near the oral apparatus of the attachment site of a microtubule filament bundle, fibrous interconnections, and a rod near a basal body that is out of the section plane in this TEM. Discoidal vesicles probably involved in digestion are aligned along the inside edge of the rod as are the extrusive organelles, the toxicysts. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 5/28/69 by R. Allen with Philips 300 operating at 60kV. Neg. 14,800X. The raw film was scanned with an Epson Perfection V750 Pro. This high resolution image is suitable for quantitative analysis.

Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 3244

• Coleps hirtus

• Actin cytoskeleton
• Cellular ingestive element
• Ciliated Protist
• Cytoskeleton
• Eukaryotic cell
• Eukaryotic Protist
• Microtubule basal body
• Microtubule cytoskeleton
• Plasma membrane

• Cambridge Culture Collection, Cambridge, England, catalog # LB1656/1
• Digestive system process
• Microfibril organization
• Microtubule cytoskeleton organization
• Structural molecule activity

• data
• image

• No linguistic content; Not applicable

Samplenumber: 9018

Doi: https://doi.org/10.6075/J0W37VB1

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL9018

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12