Allen, Richard (2021). CIL:2872, Coleps hirtus, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0N29W06

A high resolution image of a longitudinal section of an oral basal body and cilium with the attachment of a bundle of microtubular filaments and a rod that covers the proximal end of the basal body. A layer of filaments underlies the corrugated lips and lamellae extend from these lips. The oblique section through the bundle of microtubules exposes the periodic appearance of the bundle. Parasomal sacs, discoidal vesicles, mucocysts, early endosomes, and toxicysts are seen in this cortical section. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 5/28/69 by R. Allen with Philips 300 operating at 60kV. Neg. 14,800X. The raw film was scanned with a Nikon Coolscan 9000ED. This high resolution image is suitable for quantitative analysis

Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 3242

• Coleps hirtus

• Actin cytoskeleton
• Axonemal dynein complex
• Axonemal microtubule
• Ciliated Protist
• Cilium axoneme
• Cytoskeleton
• Eukaryotic cell
• Eukaryotic Protist
• Microtubule basal body
• Microtubule cytoskeleton
• Plasma membrane

• Aid to ingestion
• Cambridge Culture Collection, Cambridge, England, catalog # LB1656/1
• Ciliary or flagellar motility
• Microfibril organization
• Microtubule cytoskeleton organization
• Motility
• Structural constituent of cytoskeleton
• Structural molecule activity

• data
• image

• No linguistic content; Not applicable

Samplenumber: 2872

Doi: https://doi.org/10.6075/J0N29W06

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL2872

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12