Allen, Richard (2021). CIL:36756, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0MP527S

A grazing section through the cell‚Äôs surface shows segments of six rows of cilia running across the figure sectioned superfically at the top and deeper into the cell as one progresses down the figure. Crests of the polygonal ridges appear at the top of the figure where the expanded alveoli fill most of the space in the surface depressions. Septa, the borders of adjoining alveolar sacs, run between basal body/cilium complexes along a kinety. Trichocyst tips penetrate into the lateral ridges that separate the concave depressions of a kinety. Within the cortical cytosol a more superficial system of striated bands connects the slopes of adjacent ridges, laterally, longitudinally and diagonally. Internal to the striated bands is a meshwork of centrin-containing fibrous bundles called the infraciliary lattice. This meshwork surrounds the proximal half of basal bodies but does not insert into the pellicular membranes. Collidine-buffered fixative. TEM taken on 7/8/69 by R. Allen with Philips 300 operating at 60kV. Neg. 14,800X. Bar = 0.5µm.

• 2021

• Allen, Richard

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:38892) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed

• Cell Image Library Group ID: 9847

• Paramecium caudatum

• Cell by organism
• Cell cortex
• Ciliated Protist
• Cilium
• Cortical cytoskeleton
• Eukaryotic cell
• Eukaryotic Protist
• Microtubule basal body
• Pellicle

• Cortical cytoskeleton organization
• Plasma membrane organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 36756

Doi: https://doi.org/10.6075/J0MP527S

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36756

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12