Ethylene-free, mature-green 'Cavendish' bananas (Musa sp., AAA type) were subjected to multiple (3X) applications of an ethylene antagonist 1-methylcyclopropene (1-MCP) for 24 hrs at 20℃ to investigate the ripening response. By performing application of 1-MCP three times, it was found that a distinct ethelene evolution in treated bananas appeared at 27 days after treatment (DAT) whereas a ethelene evolution in untreated bananas appeared at 8 DAT. Single treatment was less effective in delaying ripening than repeated applications but the concentrations of 100 and 500 nℓ/ℓ of 1-MCP affected the retardation to the same extent. Regardless of ripening period, the fruit firmness and breakdown of starch to sugar at the ripe stage were almost at the same level in both treated and untreated bananas, although some bananas changed abnormally in peel color. It is often observed that the untreated bananas demonstrated ethelene evolution at 4 DAT, much earlier than expected, thus we investigated the 1-MCP response of the bananas possibly contaminated by ethylene during transit or storage. The simultaneous presence of ethelene affected the effectiveness of 1-MCP treatment. At ≤ 0.5 μℓ/ℓ ethelene, 20 nℓ/ℓ 1-MCP was sufficient to obtain almost maximum delay in ripening. However, at 1.0 μℓ/ℓ ethelene, 100 nℓ/ℓ 1-MCP was necessary to obtain the maximum delay. These indicate a competitive relationship between ethelene and 1-MCP, the effect of exposure to ethelene during transit or storage could be canceled out by using higher concentrations of 1-MCP.