2004 Volume 72 Issue 1 Pages 32-34
Rapid detection system for nitrifying bacteria was developed using photon correlation spectroscope (PCS) technique. The method is based on that nitrifying bacterial cells reacted with anti-nitrifying bacteria antibody were able to analyze by PCS technique. When nitrifying bacterial cells were reacted with the antibody beads, the size distribution was shifted to larger range. In contrast, in the reference bacterial cells, the size distribution shows little change. The phenomenon was assumed that since the antibody reacted with nitrifying bacteria selectively, they were aggregated with bacterial cells, and the distribution size increased. Thus, it is possible to discriminate between aimed nitrifying bacteria and other species of bacterial cells using PCS technique. One assay could be completed in 10 min, and the total PCS assay time including sample preparation was within 60 min.
