Electrochemical Flow Immunoassay Using Capillary Column and Ferrocene Conjugated Immunoglobulin G

Tae-Kyu LIM; Noriyuki NAKAMURA; Masateru IKEHATA; Tadashi MATSUNAGA

doi:10.5796/electrochemistry.68.872

Communications

Electrochemical Flow Immunoassay Using Capillary Column and Ferrocene Conjugated Immunoglobulin G

Tae-Kyu LIM, Noriyuki NAKAMURA, Masateru IKEHATA, Tadashi MATSUNAGA

Author information

Keywords: Ferrocene Conjugated IgG, HCG, Electrochemical Flow Immunoassay, Capillary Column

JOURNAL FREE ACCESS

2000 Volume 68 Issue 11 Pages 872-874

DOI https://doi.org/10.5796/electrochemistry.68.872

Details

Abstract

This paper describes a miniaturized flow immunoassay system. Ferrocene (Fc) conjugated with anti-HCG immunoglobulin G (IgG) antibody (Fc-IgG) was prepared and used as a novel analytical reagent. The system consists of the immunoreaction part, the capillary column packed with cation exchange resin, and the flow cell for electrochemical detection of Fc-IgG. The assay yielded a linear relationship between signal and HCG concentration in the range of 0-2000 mIU/mL (L=dm³). This electrochemical flow immunoassay requires minute quantities of serum and generates highly reproducible results.

Corresponding author