Status of Antioxidant Enzymes in Normal Cycling and α-Tocopherol Supplemented Anestrus Buffalo Heifers (Bubalus bubalis) |
R. S. Kahlon, Rajvir Singh |
|
|
|
|
Abstract |
The present investigation was undertaken to study status of erythrocytic antioxidant enzymes in normal cycling and ??tocopherol supplemented anestrus buffalo heifers. The pre-supplementation erythrocytic activities of superoxide dismutase (U/mg Hb), glutathione peroxidase (U/mg Hb) and glucose-6-phosphate dehydrogenase (U/g Hb) upregulated significantly (p<0.05) in anestrus heifers (10.08??.09, 14.09??.54, 9.25??.29) when compared to normal cycling ones (6.93??.04, 11.61??.19, 5.58??.26). The oral supplementation of ??tocopherol @ 3,000 mg per week per animal in anestrus heifers declined erythrocytic superoxide dismutase and glucose-6-phosphate dehydrogenase activities significantly (p<0.01) but led to non-significant increase in erythrocytic glutathione peroxidase activity. Results indicated that supplementation of ??tocopherol to anestrus buffalo heifers mitigated the effects of oxidative stress to improve their antioxidant status. |
Keywords:
Antioxidant Enzyme; a-Tocopherol; Anestrus; Buffalo Heifers |
|