Size Estimation of Biological Ink Particles Dispersed in Liquids Using Atomic Force Microscopy

Toshihiko Matsuura; Takamine Kato; Makoto Horii; Shohei Todo; Ken-ichi Minato; Takashi Ueno

doi:10.56646/jjapcp.1.0_011003

20th International Colloquium on Scanning Probe Microscopy

Session ID : 011003

DOI https://doi.org/10.56646/jjapcp.1.0_011003

Conference information

Host: The Japan Society of Applied Physics

Name : 20th International Colloquium on Scanning Probe Microscopy

Location : Okinawa, Japan

Date : December 17, 2012 - December 19, 2012

Size Estimation of Biological Ink Particles Dispersed in Liquids Using Atomic Force Microscopy

Toshihiko Matsuura, Takamine Kato, Makoto Horii, Shohei Todo, Ken-ichi Minato, Takashi Ueno

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CONFERENCE PROCEEDINGS OPEN ACCESS

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Abstract

The size distributions of size-controlled ink particles isolated from the ink sacs of squid and cuttlefish were studied by atomic force microscopy (AFM). Topological images indicated that the squid ink particles deposited on mica were not spherical but instead were hemisphere-like. From the heights and widths of the hemisphere-like structures on mica, the diameters of the squid ink particles dispersed in aqueous suspension were estimated to be approximately nm, close to that obtained from dynamic light scattering analysis. The estimated values were almost the same regardless of the spring constant of AFM cantilevers. A precise estimation was also achieved for the cuttlefish ink particles. The estimation method suggested in the present study would be a valid technique for precisely measuring the size distribution of biological particles by AFM.

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