Expression of mRNAs for DNA-methyltransferases and histone deacetylases in granulosa cells and follicular fluid of women undergoing in vitro fertilization: A pilot study
1 Doctoral School of Health Sciences, Faculty of Health Sciences, University of Pécs, Pécs, Hungary.
2 National Laboratory for Human Reproduction, University of Pécs, 7624Pécs, Hungary.
3 Department of Public Health Medicine, Medical School, University of Pécs, Pécs, Hungary.
4 ELKH-PTE Human Reproduction Scientific Research Group, 7624 Pécs, Hungary.
5 Department of Obstetrics and Gynaecology, Medical School, University of Pécs, Pécs, Hungary.
Research Article
International Journal of Life Science Research Archive, 2023, 04(01), 069-079.
Article DOI: 10.53771/ijlsra.2023.4.1.0015
Publication history:
Received on 08 December 2022; revised on 19 January 2023; accepted on 21 January 2023
Abstract:
Background: Gene products involved in reproduction frequently undergo post-transcriptional modifications by DNA methylation and histone acetylation.
Aims: To assess the predictive value of gene expression levels of DNA methyltransferases (DNMTs) and histone deacetylases (HDACs) in patients treated with in vitro fertilization (IVF).
Study design: Prospective, l clinical study.
Subject and methods: 31 consecutive patients with male (n=17) or female (n=14) infertility diagnoses were enrolled. Granulosa cells (GCs) and follicular fluid (FF) were obtained at the oocytes retrieval during IVF. MRNA levels of DNMT1, DNMT3a, DNMT3b and HDAC5, HDAC6 were measured in GCs and FF by quantitative RT-PCR using ROCHE Lightcycler 480.
Outcome measures: Number of oocytes retrieved, mature oocytes and viable embryos, as well as chemical and clinical pregnancy.
Results: It was demonstrated that genes for DNMTs and HDACs could be detected in nearly equal amount in GC and FF, however, only the DNMT3a transcript in FF correlated with that in GC (r=0.478, p<0.033). Moreover, FF DNMT3a was significantly higher in the pregnant (N=9) than in the non-pregnant (N=22) patients (p<0.016), and HDAC6 in GC was significantly related to the number of oocytes retrieved (r=0.413, p<0.026), MII oocytes (r= 0.383, p<0.040) and viable embryos (r=0.413, p<0.025).
Conclusions: In our clinical setting the expression of mRNA for FF DNMT3a and for GC HDAC6 has the potential to assess IVF outcome.
Keywords:
DNA-methyltransferases; Follicular fluid; Granulosa cells; Histone deacetylases; In vitro fertilization; MRNA expression
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