Antagonism between Trichoderma harzianum, T.viride and some species of the nematode fungi on solid media

Authors

  • Ali A Kasim College of Science/University of Misan
  • Anfal A College of Science/University of Misan

DOI:

https://doi.org/10.52834/jmr.v16i31.31

Keywords:

Trichoderma harzianum, T. viride, Nematode-Trapping Fungi

Abstract

      The antagonism between Trichoderma harzianum, T.viride and seven species of nematode trapping fungi (Arthrobotrys oligospora, A.conoides, A.udermata, A.thaumasia, A.microcaphoides, A.cookedichison and Clonostachys rosea) were  evaluated on solid media (potato dextrose agar (PDA) and Corn Meal Agar (CMA)). The results showed that T.harzianum and T.viride revealed a high antagonism capacity against the tested nematode trapping fungi. It was found that T.rhizianum showed the highest antagonism ability comparing with T.viride. both species gave antagonism degrees 1, 2, 3 according to the Bell et al. (1982), and 2 antagonism degree was the most apparent on both CMA and PDA media. The results showed that there was no significant difference in the degree of antagonism when using CMA and PDA except for some of the fungi that had a difference between the two media. It was noted that the T.harzianum had 1 antagonism degree with A.conoides fungi and on the CMA and PDA and A.thaumasia fungi on the CMA, while the T.viride gave the same degree with A.eudermata on the PDA.

References

الساعدي، احمد مشاري ذاري. 2011. إمكانية تصنيع مستحضر احيائي من الفطر Trichoderma viride وتطبيقه في المكافحة الاحيائية لمرض ذبول وتعقد جذور البطيخ المتسبب عن الفطر Fusarium oxysporum f.sp.melonis والنيماتود Meloidogyne spp.، رسالة ماجستير، كلية الزراعة، جامعة البصرة.

قاسم، علي عبدالواحد. 1997. دراسة حول الفطريات المهلكة للنيماتود في تربة جنوب العراق. رسالة ماجستير، جامعة البصرة، 101.

Bhardwaj, N. R. and J. Kumar . 2017. Characterization of volatile secondary metabolites from Trichoderma asperellum. J. Appl. & Nat. Sci. 9 (2): 954 - 959.

Dong, L. Q., and Zhang, K. Q. (2006). Microbial control of plant-parasitic nematodes: A five-party interaction. Plant and Soil, 288, 31–45.

Gouveia, S. A., F. E. F. Soares, T. Morgan, B. L. Sufiate, G. P. Tavares, and F. R. Braga. 2017. “Enhanced Production of Monacrosporium thaumasium Protease and Destruction Action on Root-Knot Nematode Meloidogyne Javanica Eggs.” Rhizosphere 3 (P1): 13–15. https://doi.org/10.1016/j.rhisph.2016.12.001.

Dix NJ, Webster J. 1995 – Fungal ecology. London. Chapman & Hall.

Duffy B, Schouten A, Raaijmakers JM. 2003 – Pathogen self-defense: mechanisms to counteract microbial antagonism. Annual Review of Phytopathology 41, 501–538, Doi:10.1146/annurev. phyto.41.052002.095606.

Harman, G. E. 2000. “Myths and Dogmas of Biocontrol Changes in Perception Derived from Research on Trichoderma Harzianum T-22.” Plant Disease 84: 377–393.

Herrera-estrella, A., and S. Casas-flores. 2016. “Nematophagous Fungi.” In Environmental and Microbial Relationships, edited by I.S. Druzhinina and C.P. Kubicek, 3rd Editio, 247–67. Switzerland: Springer International.

kader, M. A. Al. 2008. “In Vitro Studies on Nematode Interactions with Their Antagonistic Fungi in the Rhizosphere of Various Plants.” Albert-Ludwigs-Universität Freiburg im Breisgau, Germany.

Klepzig, K. D., Moser, J. C., Lombardero, M. J., Ayres, M. P., Hofstetter, R. W. and Walkinshaw, C. J. 2001. Mutualism and Antagonism: Ecological Interactions Among Bark Beetles, Mites and Fungi. Biotic Interactions in Plant-Pathogen Associations (eds M.J. Jeger and NJ. Spence) p.237-267.

Lafta, Anfal A. and Kasim, A. A. 2019. Effect of Nematode-trapping fungi, Trichoderma harzianum and Pseudomonas fluorescens in controlling Meloidogyne spp. Plant Archives. 19 (1) : 1163-1168.

Lee, J., Huh, N., Hong, J. H., Kim, B. S., Kim, G. H. and Kim, J. J. 2012. The antagonistic properties of Trichoderma spp. inhabiting woods for potential biological control of wood-damaging fungi. Holzforschung, Vol. 66, pp. 883–887, DOI 10.1515/HF.2011.187

Patkowska1, E., Błażewicz-Woźniak M. and Konopiński M. 2015. Antagonistic activity of selected fungi occurring in the soil after root chicory cultivation. Plant Soil Environ. Vol. 61, 2015, No. 2: 55–59. doi: 10.17221/920/2014-PSE.

Reino, J. L., R. F. Guerrero, Hernandez G. R., and I. G. Collado. 2008. “Secondary Metabolites from Species of the Biocontrol Agent Trichoderma Secondary Metabolites from Species of the Biocontrol Agent Trichoderma.” Phytochem Rev 7: 89–123.

Suresh, N. and Nelson, R. 2016. Isolation of antagonistic fungi and evaluation of antifungal activity of the separated metabolite against the red rot of sugarcane pathogen. European Journal of Experimental Biology, 6(1): 15-21.

Szabó, M., K. Csepregi, M. Gálber, F. Virányi, and C. Fekete. 2012. “Control Plant-Parasitic Nematodes with Trichoderma Species and Nematode-Trapping Fungi: The Role of Chi18-5 and Chi18-12 Genes in Nematode Egg-Parasitism.” Biological Control 63 (2): 121–28.

Verma. M., Brar, S. K., Tyagi, R.D., Surampalli, R.Y. and Val´ero, J.R. 2007. Antagonistic fungi, Trichoderma spp.: Panoply of biological control. Biochemical Engineering Journal 37.1–20.

Vinale, F., Sivasithamparam, K., Ghisalberti, E.L., Marra, R., Woo, S.L., Lorito, M.,2008. Trichoderma–plant–pathogen interactions. Soil Biology and Biochemistry 40, 1–10.

Waing KGD, Abella EA, Kalaw SP, Waing FP, Galvez CT 2015 – Antagonistic interactions among different species of leaf litter fungi of Central Luzon State University. Plant Pathology & Quarantine 5(2), 122–130, Doi 10.5943/ppq/5/2/9

Published

2020-05-31

How to Cite

قاسم ع. ع. . . . ., & الرحيماوي أ. ع. (2020). Antagonism between Trichoderma harzianum, T.viride and some species of the nematode fungi on solid media. Journal of Misan Researches, 16(31), 266-276. https://doi.org/10.52834/jmr.v16i31.31

Issue

Section

Articals