Morphological determination of localization and function of Golgi proteins

Yusheng Xing; Yannan Jian; Xiaodan Zhao; Yue Zhang; Zhenqian Zhang; Xing Zhang; Xiaoyan Zhang

doi:10.52601/bpr.2024.240008

Volume 10 Issue 2

Apr. 2024

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Yusheng Xing, Yannan Jian, Xiaodan Zhao, Yue Zhang, Zhenqian Zhang, Xing Zhang, Xiaoyan Zhang. Morphological determination of localization and function of Golgi proteins. Biophysics Reports, 2024, 10(2): 121-132. doi: 10.52601/bpr.2024.240008

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Yusheng Xing, Yannan Jian, Xiaodan Zhao, Yue Zhang, Zhenqian Zhang, Xing Zhang, Xiaoyan Zhang. Morphological determination of localization and function of Golgi proteins. Biophysics Reports, 2024, 10(2): 121-132. doi: 10.52601/bpr.2024.240008

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Morphological determination of localization and function of Golgi proteins

doi: 10.52601/bpr.2024.240008

1.
College of Biomedicine and Health, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China

More Information

Corresponding author: xiaoyanz@mail.hzau.edu.cn (X. Zhang)
Received Date: 31 January 2024
Accepted Date: 21 February 2024

Available Online: 26 April 2024

Publish Date: 30 April 2024

Abstract

Abstract

In animal cells, the Golgi apparatus serves as the central hub of the endomembrane secretory pathway. It is responsible for the processing, modification, and sorting of proteins and lipids. The unique stacking and ribbon-like architecture of the Golgi apparatus forms the foundation for its precise functionality. Under cellular stress or pathological conditions, the structure of the Golgi and its important glycosylation modification function may change. It is crucial to employ suitable methodologies to study the structure and function of the Golgi apparatus, particularly when assessing the involvement of a target protein in Golgi regulation. This article provides a comprehensive overview of the diverse microscopy techniques used to determine the specific location of the target protein within the Golgi apparatus. Additionally, it outlines methods for assessing changes in the Golgi structure and its glycosylation modification function following the knockout of the target gene.
- Golgi apparatus,
- Golgi fragmentation,
- Immunofluorescence,
- Super-resolution microscopy,
- Electron microscopy,
- Lectin staining,
- Glycosylation

Yusheng Xing, Yannan Jian, Xiaodan Zhao, Yue Zhang, Zhenqian Zhang, Xing Zhang and Xiaoyan Zhang declare that they have no conflict of interest.
This article does not contain any studies with human or animal subjects performed by the any of the authors.
^#usheng Xing, Yannan Jian and Xiaodan Zhao contributed equally to this work.

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