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Phytofabrication of silver nanoparticles using callus extracts of natural tetraploid Trifolium pratense L. and its bioactivities

Year 2023, Current Perspectives in Modern Biology: Exploring Diverse Frontiers, Paradigms, and Novel Horizons, 18 - 28, 31.12.2023

Havva Karahan Nurten Tetik Hatice Çölgeçen

https://doi.org/10.51753/flsrt.1357092

Abstract

One of the main subjects of plant biotechnology is plant tissue culture and in recent years is considered a possible approach model for green and eco-friendly biosynthesis of nanoparticles. This study aimed to present calli produced from the natural tetraploid Trifolium pratense L. containing high amounts of phenolic compounds and glycosidic bioactive macromolecules and the biosynthesis of silver nanoparticles from calli. Combinatorial optimization of silver nanoparticles was achieved for the first time in this study, thanks to the stabilizing and reducing properties of hypocotyl, apical meristem, and epicotyl derived callus extracts of the natural tetraploid T. pratense L. biosynthesized nanoparticles from three different callus extracts. Callus extracts were used to create different experiments with AgNO3 at various concentrations (0.16, 0.5, 0.84, 1.18, 1.52 and 1.96 mg L-1), different temperatures (40, 50, 60, 70, 80, 90, 100°C), and different pH levels (5, 7, 10) to carry out the biosynthesis of AgNPs. Biologically synthesized AgNPs were easily monitored by color change in ultraviolet and UV-Vis spectroscopy proved to be a fast and simple method. Also, TEM, XRD, and FTIR analyses were done to characterize and confirm the formation of crystalline nanoparticles. It was determined that antibacterial activity inhibition was achieved by using the Agar-well diffusion method for antibacterial activity measurements on Gram-positive Staphylococcus aureus ATCC 25923 and Gram-negative Escherichia coli CECT 4972 bacteria. Biosynthesized AgNPs were observed in the wavelength range of 400-500 nm in the UV-VIS spectrum. TEM analysis demonstrated the size and shape of biosynthesized silver nanoparticles under different conditions. It was observed that the smaller silver nanoparticles were spherical and the larger silver nanoparticles were triangular, elliptical, and spherical shape. The XRD analysis proved the presence of Ag0 in nanoparticles and showed crystal structure for silver nanoparticles. By FTIR analysis, O-H hydroxyl groups of functional groups on the AgNP surface, H-linked OH stretching, C-H stretching, -CH stretching of -CH2 and -CH3 functional groups, C-N and carboxylate, aliphatic phosphate and primary amine stretching were expressed. Biosynthesized silver nanoparticles showed antibacterial activity against Gram-positive S. aureus ATCC 25923 bacteria, AgNP hypocotyl (1.7mm), AgNP-epicotyl (1.1mm) against Gram-negative E. coli CECT 4972 bacteria. Among the hypocotyl, apical meristem, and epicotyl callus cultures, the highest antioxidant activity was observed in the AgNPs obtained from hypocotyl-concentration experiments, with a DPPH radical activity of 52% and an ABTS radical activity of 68%. In conclusion, these findings underscore the potential of biotechnological strategies in green nanotechnology, which can be offered for developing metal nanoparticles with potential biomedicine and biotechnology applications.

Keywords

Antibacterial activity antioxidant activity callus culture characterization reducing and stabilizing agents silver nanoparticles

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Year 2023, Current Perspectives in Modern Biology: Exploring Diverse Frontiers, Paradigms, and Novel Horizons, 18 - 28, 31.12.2023

Havva Karahan Nurten Tetik Hatice Çölgeçen

https://doi.org/10.51753/flsrt.1357092

Abstract

References

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  • Alfarraj, N. S., Tarroum, M., Al-Qurainy, F., Nadeem, M., Khan, S., Salih, A. M., ... & Perveen, K. (2023). Biosynthesis of Silver Nanoparticles and Exploring Their Potential of Reducing the Contamination of the In Vitro Culture Media and Inducing the Callus Growth of Rumex nervosus Explants. Molecules, 28(9), 3666.
  • Allawadhi, P., Singh, V., Khurana, A., Khurana, I., Allwadhi, S., Kumar, P., ... & Bharani, K. K. (2021). Silver nanoparticle based multifunctional approach for combating COVID-19. Sensors International, 2, 100101.
  • Anjum, S., & Abbasi, B. H. (2016). Thidiazuron-enhanced biosynthesis and antimicrobial efficacy of silver nanoparticles via improving phytochemical reducing potential in callus culture of Linum usitatissimum L.. International Journal of Nanomedicine, 11, 715-728.
  • Aref, M. S., & Salem, S. S. (2020). Bio-callus synthesis of silver nanoparticles, characterization, and antibacterial activities via Cinnamomum camphora callus culture. Biocatalysis and Agricultural Biotechnology, 27, 101689.
  • Arsène, M. M. J., Podoprigora, I. V., Davares, A. K. L., Razan, M., Das, M. S., & Senyagin, A. N. (2021). Antibacterial activity of grapefruit peel extracts and green-synthesized silver nanoparticles. Veterinary World, 14(5), 1330-1341.
  • Avcioglu, F., Behçet, M., Karabork, S., & Kurtoglu, M. G. (2019). Yara örneklerinden izole edilen Mikroorganizmaların Antimikrobiyal direnç oranları-üç yıllık değerlendirme. Düzce Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi, 9(3), 110-114.
  • Labulo, A. H., Adesuji, E. T., Dedeke, O. A., Bodede, O. S., Oseghale, C. O., Moodley, R., ... & Adegoke, O. A. (2016). A dual-purpose silver nanoparticles biosynthesized using aqueous leaf extract of Detarium microcarpum: an under-utilized species. Talanta, 160, 735-744.
  • Aziz, M. S. A., Shaheen, M. S., Nekeety, A. A., & Wahhab, M. A. A. (2014). Antioxidant and antibacterial activity of silver nanoparticles biosynthesized using Chenopodium murale leaf extract. Journal of Saudi Chemical Society, 18, 356-363.
  • Baran, M. F., Keskin, C., Baran, A., Hatipoğlu, A., Yildiztekin, M., Küçükaydin, S., ... & Eftekhari, A. (2023). Green synthesis of silver nanoparticles from Allium cepa L. Peel extract, their antioxidant, antipathogenic, and anticholinesterase activity. Molecules, 28(5), 2310.
  • Baruah, K., Haque, M., Langbang, L., Das, S., Aguan, K., & Roy, A.S. (2021). Ocimum sanctum mediated green synthesis of silver nanoparticles: A biophysical study towards lysozyme binding and anti-bacterial activity. Journal of Molecular Liquids, 337, 116422.
  • Bedlovičová, Z., Strapáč, I., Baláž, M., & Salayová, A. (2020). A brief overview on antioxidant activity determination of silver nanoparticles. Molecules, 25(14), 3191.
  • Bernabé-Antonio, A., Martínez-Ceja, A., Romero-Estrada, A., Sánchez-Carranza, J. N., Columba-Palomares, M. C., Rodríguez-López, V., ... & Gutiérrez-Hernández, J. M. (2022). Green synthesis of silver nanoparticles using Randia aculeata L. cell culture extracts, characterization, and evaluation of antibacterial and antiproliferative Activity. Nanomaterials, 12(23), 4184.
  • Botcha, S., & Prattipati, S. D. (2020). Callus extract mediated green synthesis of silver nanoparticles, their characterization and cytotoxicity evaluation against MDA-MB-231 and PC-3 cells. BioNanoScience, 10, 11-22.
  • Buyukkartal, H. N., & Colgecen, H. (2007). The reasons of sterility during pollen grain formation in the natural tetraploid Trifolium pratense L. International Journal of Botany, 3(2), 188-195.
  • Chowdhury, M. H., Ray, K., Geddes, C. D., & Lakowicz, J. R. (2008). Use of silver nanoparticles to enhance surface plasmon-coupled emission (SPCE). Chemical Physics Letters 452, 162-167.
  • Chowdhury, R. A., Dhar, S. A., Das, S., Nahian, K., & Qadir, R. (2021). Green synthesis and characterization of silver nanoparticles from the aqueous extract of the leaves of Citrus aurantifolia. Materials Today: Proceedings, 44, 1039-1042.
  • CLSI, (2020). Performance standards for antimicrobial susceptibility testing. 30th ed. CLSI supplement M100. Wayne, PA: Clinical and Laboratory Standards Institute.
  • Colgecen, H., Calıskan, U., Kartal, M., & Buyukkartal, H. N. (2014). Comprehensive evaluation of phytoestrogen accumulation in plants and in vitro cultures of Medicago sativa L. 'Elçi' and natural tetraploid Trifolium pratense L.. Turkish Journal of Biology, 38, 619-627.
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Details

Primary Language English
Subjects Plant Biotechnology
Journal Section Research Articles
Authors

Havva Karahan 0000-0003-0518-6265

Nurten Tetik 0000-0002-1883-1166

Hatice Çölgeçen 0000-0001-8246-4279

Publication Date December 31, 2023
Submission Date September 8, 2023
Published in Issue Year 2023 Current Perspectives in Modern Biology: Exploring Diverse Frontiers, Paradigms, and Novel Horizons

Cite

APA Karahan, H., Tetik, N., & Çölgeçen, H. (2023). Phytofabrication of silver nanoparticles using callus extracts of natural tetraploid Trifolium pratense L. and its bioactivities. Frontiers in Life Sciences and Related Technologies18-28. https://doi.org/10.51753/flsrt.1357092
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