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Nano Biomedicine and Engineering 2019, 11(4): 333-339 https://doi.org/10.5101/nbe.v11i4.p333-339
Research Article | Open Access | Issue | Published: 02 November 2019

Electrochemical Determination of Japanese Encephalitis Virus Antigen Using Silver Nanoparticles Modified Screen-Printed Carbon Electrode

Show Author's Information Suk Fun Chin1( ),  Lih Shan Lim1,  Huat Choi Lai1,  Suh Cem Pang1,  Magdline Sia Henry Sum2,  David Perera2
Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia
Institute of Health and Community Medicine, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia
Keywords:
Electrochemical impedance spectroscopy, Silver nanoparticle, Screen printed carbon electrode, Japanese encephalitis virus antigen
Cite this article:
Chin SF, Lim LS, Lai HC, et al. Electrochemical Determination of Japanese Encephalitis Virus Antigen Using Silver Nanoparticles Modified Screen-Printed Carbon Electrode. Nano Biomedicine and Engineering, 2019, 11(4): 333-339. https://doi.org/10.5101/nbe.v11i4.p333-339
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Abstract Full text About this article

In this study, we aim to fabricate a silver nanoparticles (Ag NPs) based electrochemical biosensor for Japanese Encephalitis virus antigen detection. Ag NPs were deposited onto screen-printed carbon electrode (SPCE) and the electrochemical properties of the Ag NPs modified SPCE were investigated via electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). It was observed that the deposition of Ag NPs onto electrode surface has significantly enhanced the conductivity up to 20.5% than that of bare SPCE. The EIS data indicated limit of detection (LOD) of 2.60 ng/mL (at S/N = 3) towards JEV antigen, with an analysis assay time of 20 min. This presented Ag NPs modified SPCE has demonstrated a promising and rapid alternative to conventional biosensing techniques towards JEV antigen.


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Electrochemical Determination of Japanese Encephalitis Virus Antigen Using Silver Nanoparticles Modified Screen-Printed Carbon Electrode

Show Author's information Suk Fun Chin1( ), Lih Shan Lim1, Huat Choi Lai1, Suh Cem Pang1, Magdline Sia Henry Sum2, David Perera2
Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia
Institute of Health and Community Medicine, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia

Abstract

In this study, we aim to fabricate a silver nanoparticles (Ag NPs) based electrochemical biosensor for Japanese Encephalitis virus antigen detection. Ag NPs were deposited onto screen-printed carbon electrode (SPCE) and the electrochemical properties of the Ag NPs modified SPCE were investigated via electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). It was observed that the deposition of Ag NPs onto electrode surface has significantly enhanced the conductivity up to 20.5% than that of bare SPCE. The EIS data indicated limit of detection (LOD) of 2.60 ng/mL (at S/N = 3) towards JEV antigen, with an analysis assay time of 20 min. This presented Ag NPs modified SPCE has demonstrated a promising and rapid alternative to conventional biosensing techniques towards JEV antigen.

Keywords: Electrochemical impedance spectroscopy, Silver nanoparticle, Screen printed carbon electrode, Japanese encephalitis virus antigen

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Publication history
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Publication history

Received: 12 September 2019
Accepted: 02 November 2019
Published: 02 November 2019
Issue date: December 2019

Copyright

© Suk Fun Chin, Lih Shan Lim, Huat Choi Lai, Suh Cem Pang, Magdline Sia Henry Sum, and David Perera.

Acknowledgements

Acknowledgements

Financial support by the Ministry of Education (MOE) through the Exploratory Research Grant Scheme (ERGS), Grant no. ERGS/STG05(01)/1005/2013(02), F07/(DPP01)/1121/2014 and graduate scholarships from MyBrain15 (MyMaster) Programme, Universiti Malaysia Sarawak Zamalah, MyBrain15(MyPhD) Programme are gratefully acknowledged.

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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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