The development of laser-driven neutron source (LDNS) utilizing laser-accelerated ions and its application to several kinds of neutron analysis performed at LFEX facility of ILE Osaka University. The LDNS generates neutrons in the wide range of energy from meV to MeV. The main argument of this review is to demonstrate “Single-Shot” neutron analysis by LDNS, where one analysis is accomplished by a single bunch of neutrons generated with a single laser shot. This method enables the high-speed analysis of nuclear information including elements and isotopes.