In order to develop an ‘in vivo model’ to examine the direct effect of drugs to bone tissue, we conducted the following experiment in Japanese white rabbits. Under anesthesia we drilled a hole, 4mm in diameter, in the distal epiphyseal segment of the bilateral femurs. After 4, 7, 14 and 28 days, the rabbits were killed, and radiographic and histological examinations were performed. After 4 days, new bone formation was observed histologically at the marginal area of the drilled cavity and after 28 days, the cavity was almost filled with new bone. Fibrin Sealant was absorbed gradually and disappeared completely after 28 days.
Two holes of each rabbit were filled, one with the mixture of calcitonin-Fibrin Sealant, another with Fibrin Sealant only. The result of this experiment showed that after 14 days, no significant difference in bone regeneration between these two holes could be found, but after 28 days, the amount of new bone tended to be larger in the hole filled with calcitonin-Fibrin Sealant than in that of Fibrin Sealant only.