Document Type : Original Article
Authors
- Debaraj B. Pallai 1
- Raj R. Badekar 2
- Kalimoddin I. Momin 3
- Abhay S. Bondge 4
- Ganpat R. Nagargoje 5
- Prasad D. Kadam 6
- Sharad P. Panchgalle 7
- Vijaykumar S. More 8
1 Delicare Life Sciences Badlapur - 421503, Dist. Thane, (M.S.), India
2 Riva Industries, Karjat - 410201, Dist. Raigad, (M.S.), India
3 Department of Chemistry, Rajarshi Shahu Mahavidyalaya, Latur - 413512, Dist. Latur, (M.S.), India
4 Department of Chemistry, Shivaneri Mahavidyalaya Shirur Anantpal – 413544, Dist. Latur, (M.S.), India
5 Department of Chemistry, Shivaji Mahavidyalaya, Renapur - 413527, Dist. Latur, (M.S.), India
6 Department of Chemistry, Shri Kumarswami Mahavidyalaya, Ausa – 413520, Dist. Latur, (M.S.), India
7 Department of Chemistry, K. M. C. College, Khopoli -410203, Dist. Raigad, (M.S.), India
8 Department of Chemistry, Kai. Rasika Mahavidyalaya Deoni - 413519, Dist. Latur, Maharashtra, India
Abstract
Electronic absorption spectra, infrared spectroscopy, nuclear magnetic resonance, molar conductance, and elemental analysis are some of the physicochemical methods used to characterize the 4-chloro-N'-[(E)-(2,6-dihydroxy phenyl) methylidene] benzohydrazide (HBHDH) molecule that has been synthesized. The results of the analysis show that the metal and ligand formed a 1:2 ratio. According to the spectra, the ligand HBHDH forms tridentate coordination spheres with metal ions using an ONO bond. Complexes with Fe(II), Co(II), Ni(II), Cu(II), and Mn(II) have an octahedral shape because of this. In contrast to the tetrahedral structure of the complexes created with Zn(II), Cd(II), and Hg(II), the Pd(II) complex takes on a square planar layout. According to the molar conductance values in DMF, the complexes do not undergo electrolysis. It has been determined that the metal complexes and Schiff's base HBHDH ligand have antibacterial action. The antibacterial efficiency of each combination and ligand against E. coli bacteria is higher than that of regular streptomycin.Candida albicans (MCC 1439) and Saccharomyces cerevisiae (MCC 1033) have significant antifungal effectiveness, with their growth being inhibited by over 92% in the presence of Mn(II) and Fe(II) complexes, respectively.
Graphical Abstract
Keywords
Main Subjects
Introduction
In particular, hydrazones -NH-N=CRR′ (where R and R′ = H, alkyl, aryl) have attracted a lot of attention as a family of ligands due to their ability to create stable complexes with most metal ions through novel coordination mechanisms [1-4]. Catalytic processes and simulations of biological systems have made use of hydrazone Schiff base complexes. Schiff base metal complexes with nitrogen and other donors have recently been the subject of a great deal of chemical investigation. Their biological activity and stability, like their anti-convulsant and anti-tumor properties, may be related to osteoporosis. Oxidation catalysis and electrochemistry are two of the many possible uses for hydrazones [5,6]. Hydrazones have multiple uses, including as growth regulators, nematocides, rodenticides, and herbicides [7]. According to literature [8-10], much of the research has focused on hydrazone complexes of transition metal ions.
Hydrazine Schiff base metal complexes prepared from 4-chlorobenzohydrazide and 2, 4-dihydroxybenzaldehyde have not yet been documented in any study. The objective of this study is to create and characterize complexes of 4-chloro-N'-[(E)-(2,6-dihydroxyphenyl)methylidene]benzohydrazide (HBHDH) with several metals. Spectroscopic methods (electronic absorption, infrared, 1H nuclear magnetic resonance) and magnetic moments are used to investigate the complexes’ shape, bond types, and probable chelation mechanism. Antimicrobial and antifungal activity testing was performed on all of the synthesized compounds.
Experimental
The analytical grade (AR) chemicals and solvents used to synthesize the HBHDH ligand and its related metal complexes were supplied by S.D. Fine. To prepare metal complexes, certain metal salts are required, such as FeSO4, CoCl2·6H2O, NiCl2·7H2O, PdCl2, CuCl2·5H2O, ZnCl2·7H2O, CdCl2, HgCl2, and MnCl2·2H2O. You can buy them and use them just the way they are. The production of 4-chlorobenzohydrazide [11] followed the steps outlined in the published literature. We found the amounts of metals and chlorides using Vogel's approach [12]. The ligand and its metal complexes were studied by measuring their infrared spectra with KBr pellets using a Brucker infrared spectrophotometer. A JASCO UV-Visible spectrophotometer (V-650) was used to detect the electronic absorption spectra of the metal complexes within the 1000 to 200 nm wavelength range. Researchers recorded the 1H-NMR spectra of the ligand and its metal complexes in DMSO-d6 using a Bruker 400 MHz spectrophotometer, with TMS acting as an internal reference. The method developed by Rast was used to determine molecular weights [13-17].
At room temperature, the metal complexes' magnetic susceptibilities were measured using Gouy's method, calibrated with Hg [Co(SCN)4], which stands for mercury (II) tetra thiocyanate cobalt (II). A solution of nitrobenzene (10-3 M) was used to determine the molar conductivity at room temperature.
Preparation of HBHDH ligand
In Scheme 1, a hot 4-chlorobenzohydrazide (1) was mixed with a hot ethanolic solution of 2, 6-dihydroxybenzaldehyde (2) dropwise while being constantly agitated. An oil bath could heat the reaction mixture for three to four hours. We filtered the yellow PPT, washed it with ethanol, and dried it under vacuum after it cooled to room temperature. Colour; yellow. M.W.; 290.70 g/mol. % yield; 69.07. M.P.; 183. % composition: C, 57.84; H, 3.81; N, 9.64; O, 16.51; S, 12.20. UV-vis (lnm); 335, 295, and 335. FT(IR) (cm-1); 3257, 2926, 3190, 2937, 1629, 1605, and 1295. 1H-NMR (d); 12.01 (-OH, C2), 11.46 (-OH, C6), 10.02 (-NH), 8.55 (-CH=), and 6.36-7.99 (Ar. protons).
Scheme 1. Preparation of HBHDH ligand
Preparation of metal complexes
The appropriate metal salt (0.05 mol) was slowly added to a 15 mL container of hot ethanolic HBHDH ligand (0.1 mol) solution while stirring continuously. Hot ethanol was used to carry out the reaction. The inclusion of 0.1 N NaOH brought the reaction mixture’s pH up to 7. The mixture was heated in an oil bath and refluxed for five to eight hours. Cooling the solution after digestion reduced its volume by half and allowed it to solidify. Following a period of drying at room temperature, the product was rinsed with ethanol. Results should range from 69 to 73%.
[Fe(BHDH)2]
Colour: Blue. M.W.: 635.24 g/mol. % yield: 73.46. M.P.: 200. % composition: C, 52.89; H, 3.75; N, 8.82; O, 15.11; S, 11.20; Fe, 8.79. UV-Vis (lnm): 613 and 503. FT(IR) (cm-1): 2926, 3152, 2840, 1584, 1525, 1233, 631, 550, and 520.
[Co(BHDH)2]
Color: brown. M.W: 640.39 g/mol. % yield: 76.44. M.P.: 203. % composition: C, 52.47; H, 3.12; N, 8.75; O, 15.00; S, 11.10; Co, 9.12. UV-Vis (lnm): 900 and 675. FT(IR) (cm-1): 2919, 3448, 2849, 1596, 1542, 1217, 653, 590, and 511.
[Ni(BHDH)2]
Colour: light green. M.W.: 640.09 g/mol. % yield: 79.13. M.P.: 204. % composition: C, 52.49; H, 3.12; N, 8.75; O, 15.00; S, 11.10; Ni, 9.17. UV-Vis (lnm): 995 and 667. FT(IR) (cm-1): 2924, 3408, 2848, 1593, 1549, 1217, 639, 618, and 532.
[Pd(BHDH)2]
Colour: Green. M.W.: 687.40 g/mol. % yield: 83.87. M.P.: 209. % composition: C, 48.48; H, 2.91; N, 8.15; O, 14.00; S, 10.33; Pd, 15.42. UV-Vis (lnm): 396, 331, and 285. FT(IR) (cm-1): 2923, 3288, 2851, 1552, 1518, 1218, 535, and 525. 1H-NMR (d); 11.51 (-OH, C6), 9.74 (-NH), 8.65 (-CH=), 6.41-8.04 (Ar. protons).
[Cu(BHDH)2]
Colour: Green. M.W.: 644.95 g/mol. % yield: 81.61. M.P.: 206. % composition: C, 52.10; H, 3.10; N, 8.68; O, 14.90; S, 11.00; Cu, 9.85. UV-Vis (lnm): 638. FT(IR) (cm-1): 2923, 3152, 2852, 1581, 1513, 1219, 595, 521, and 515.
[Zn(BHDH)2]
Colour: Yellow. M.W.: 646.76 g/mol. % yield: 77.40. M.P.: 206. % composition: C, 52.10; H, 3.10; N, 8.68; O, 14.90; S, 11.00; Zn, 9.85. UV-Vis (lnm): 332, 310, and 230. FT(IR) (cm-1): 2922, 3341, 2851, 1592, 1547, 1215, 595, and 528. 1H-NMR (d); 11.28 (-OH, C6), 9.61 (-NH), 8.48 (-CH=), 7.11-7.55 (Ar. protons).
[Cd(BHDH)2]
Colour: Yellow. M.W.: 693.81 g/mol. % yield: 81.55. M.P.: 210. % composition: C, 48.43; H, 2.88; N, 8.07; O, 13.80; S, 10.20; Cd, 16.20. UV-Vis (lnm); 340, 253. FT(IR) (cm-1): 2979, 3197, 2835, 1579, 1548, 1226, 602, and 552. 1H-NMR (d); 11.27 (-OH, C6), 10.04 (-NH), 8.65 (-CH=), 6.41-8.67 (Ar. protons).
[Hg(BHDH)2]
Colour: Yellow. M.W.: 781.40 g/mol. % yield; 69.77. M.P.: 206. % composition: C, 43.00; H, 2.56; N, 7.17; O, 12.29; S, 9.09; Hg, 25.67. UV-Vis (lnm); 340, 310, 230. FT(IR) (cm-1); 2922, 3321, 2852, 1604, 1540, 1241, 529, and 510. 1H-NMR (d); 11.56 (-OH, C6), 9.89 (-NH), 8.55 (-CH=), 6.36-7.99 (Ar. protons).
[Mn(BHDH)2]
Colour: Brown. M.W.: 634.34 g/mol. % yield: 69.88. M.P.: 202. % composition: C, 52.97; H, 3.15; N, 8.83; O, 15.10; S, 11.20; mn, 8.66. UV-Vis (lnm); 561 and 398. FT(IR) (cm-1): 2925, 3257, 2836, 1552, 1515, 1218, 618, 571, and 534.
Biological evaluation
To investigate the chemicals' efficacy against various microorganisms, including Staphylococcus aureus (MCC 2408), B. subtilis (MCC 2010), Escherichia coli (MCC 2412), Pseudomonas aeruginosa (MCC 2080), fungi (C. albicans (MCC 1439), and S. cerevisiae (MCC 1033) [16], the conventional disc-agar diffusion technique was employed. Fluconazole was considered the best treatment for Gram-negative bacteria.
This is in contrast to the usage of cycloheximide against fungi and cephalothin against Gram-positive bacteria. A 2 and 1 mg/mL solution of the compounds was prepared before testing using DMF, which did not have any inhibitory effect. The experimental medium used was PDA, which stands for potato dextrose agar and is composed of 200 g of potato infusion, 6 g of dextrose, and 15 g to measure.
Before being delicately put on incubated agar surfaces, discs of filter paper were soaked with 10 mL of a solution containing a controllable concentration of test compounds. Following incubation for 36 hours at 27 °C for bacteria and 48 hours at 24 °C for fungus, the distance between each disc and the surrounding clear zone was measured. The mean inhibition zones were then calculated.
Results and discussion
The HBHDH ligand and its complexes exhibit resistance to the negative impacts of oxygen and moisture when stored at room temperature. The complexes exhibit limited solubility in water, although they are partially soluble in ethanol and methanol and completely soluble in DMF, nitrobenzene, and DMSO solvents. Table 1 presents the analytical data for both the ligand and the complexes. The results corroborate the chemical formula of the ligand, C14H11ClN2O3, and the 1:2 metal-to-ligand stoichiometry in the complexes.
Table 1. Analytical and physicochemical data of metal complexes with the HBHDH ligand
The molar conductance values of the complexes in DMF at a concentration of 10-3 M, ranging from 9.8 to 20 ohm-1cm2mol-1, suggest that the complexes lack electrolytic properties [18].
1H-NMR study of HBHDH ligand
Table 2 lists the pertinent data for determining the structure of the HBHDH ligand from the 1H NMR spectra that were acquired in CDCl3 (suplementary file). At 12.02 ppm, the HBHDH ligand's 1H-NMR spectra revealed a singlet (S, 1H, -OH C2). The C2 position's phenolic -OH group was designated as the site of this singlet. This peak is absent in all prepared metal complexes, indicating the deprotonation of the hydroxyl group during complexation. At 11.46 ppm, there was another singlet that was identified as the proton of the C6 position's phenolic -OH group (S, 1H, -OH C6) [19-20]. The singlet was observed at 10.02 ppm, assigned as the proton of the imino-NH group. The -CH= group of the prepared ligand and its metal complexes was observed at 8.55 ppm. The multiplets seen in the range from 6.38 to 7.99 ppm are assigned by aromatic protons.
Table 2.1H-NMR spectroscopic data of the HBHDH ligand and its metal complexes
Absorption and magnetic moment spectra
The HBHDH ligand and its complexes were subjected to electronic spectra measurements in DMF (suplementary file). The projected structure of synthesized transition complexes and data on magnetic moments are displayed in Table 3, which is based on recorded electronic absorption spectra. The aromatic ring π→π* transition, the >C=O transition, and the n®π* transitions of the C=N groups were identified in the ligand's electronic absorption spectra, which showed high-energy bands at 235, 295, and 335 nm, respectively. It was considered that intra-ligand and LMCT transitions of coordinated ligands caused these bands to be moved to 230-340, 253-378, and 331-480 nm in complexes. The 5T2g → 5Eg transition, identified as occurring at 613 nm and assigned to one atom, validated the complex's octahedral geometry, and a magnetic moment value of 5.12 B.M. was determined for the Fe (II) complex. The magnetic moment values of Fe(II) complexes were 3.14 B.M. and 4.45 B.M., respectively, in the literature [21,22]. The Co(II) complex's electronic spectra display three transitions at 900 and 621 nm, which can be attributed to 4T1g (F) → 4T1g (F) and 4T2g (F) → 4T1g (P) transitions, respectively.
Table 3. HBHDH ligand electronic spectral data and metal complexes
In contrast, the Ni(II) complex exhibits three transitions at 995, 667, and 545 nm, which can be attributed to 3T2g→3T2g, 3T2g→3T1g(F), and 3T2g→3T1g(P) transitions, each of which suggests an octahedral geometry. The electronic spectra of the Cu(II) compound showed a wideband with low intensity at around 638 nm in the visible region [23], which is caused by the d-d (2B1g→2Eg) transition. All signs led to a distorted octahedral arrangement.
In the Mn(II) complex, the octahedral structure was brought about via the d-d (6A1g→5Eg, 4D, and 6A1g→5T1g) transition, which was seen as the lowest-energy band at 561 and 398 nm, respectively. The electronic spectra of the Pd(II) complex showed transitions at 396, 331, and 285 nm [25]. These alterations were given a square, flat form. Because their d10 transition is complete, complexes containing Zn(II), Cd(II), and Hg(II) do not display a d-d band.
FT(IR) spectral studies
As a result of comparing the IR spectra indicated in Table 4 with those of free HBHDH ligands, we can see the different kinds of bonds that form in complexes (suplementary file). At 3190, 1629, 1605, and 1295 cm-1, respectively, the infrared bands for n(N-H), n(C=O), n(C=N), and phenolic (C-O) are visible in the free HBHDH ligand. The wavelengths that were measured are these. At 3295 cm-1, however, a medium broad band was observed due to the o-hydroxy group’s intramolecular solid hydrogen bonding. The breadth of this band was approximately 3295 cm-1. When this band vanished in complexes, it meant that phenolic oxygen had deprotonated and was coordinating with metal ions. The phenolic (C-O) frequency decreased from 1295 cm-1 to 1215-1233 cm-1, in contrast to the ligand's frequency of 1350 cm-1, lending credence to this theory. It is worth noting that this band vanished in complexes, suggesting that metal ions coordinate with phenolic oxygen by deprotonation. The stretching frequency of (C=N), which is present in free ligands, changes to a value of 63-90 cm-1 lower [24] when complexes are present. In this case, the coordination of the metal ion with the nitrogen of the azomethine molecule is seen. The (C=O) vibrations moved to the lower side of the spectrum in all transition metal complexes, indicating that this group was involved in the complexation process [26-27].
Table 4. FT(IR) spectra of metal complexes with the HBHDH ligand
That HBHDH ligands in metal complexes are monobasic tridentate was thus proven by this finding. A band in the vicinity of 3152-3448 cm-1 is allocated to the 6-position hydroxy group of the aromatic ring by the HBHDH ligand and its complexes. It appears from this band that the complexation mechanism does not involve the 6-position hydroxyl group [28-30]. The bands observed in the 535-653, 521-618, and 510-534 cm-1 far-infrared regions can be ascribed to the n(MO), n(M), and n(MN) vibrations, respectively. The structures of complexes are allocated based on spectral investigations.
Antimicrobial study
Testing the HBHDH ligand and its compounds for antibacterial properties is shown in Table 5. The standard and its metal complexes had higher activity against all microorganisms, but the ligand HBHDH had lesser activity. The chelation that took place is the reason behind this, according to Tweedy's hypothesis of chelation.
Table 5. HBHDH ligand and its metal complexes have been studied for their antibacterial properties
Antibacterial activity
The antibacterial efficacy associated with each metal complex is nearly uniform against all bacterial strains.
The average B. subtilis has an inhibition zone of 13mm, but Cu(II), Zn(II), and Co(II) all have far larger inhibition zones of 14 mm, 13 mm, and 13 mm, respectively, against E. coli. The Hg(II) molecule had no impact on S. aureus (Figure 1).
Figure 1. Antibacterial activity of HBHDH ligand and its metal complexes
Antifungal activity
The Ni(II) complex exhibits potent antifungal activity, as demonstrated by its efficacy against C. albicans. S. cerevisiae demonstrates a growth rate reduction of over 90% in the presence of the Mn(II) complex (Table 6). The Co(II) complex inhibits the development of all fungal strains (Figure 2).
Table 6. Antifungal investigation on the HBHDH ligand and its metal complexes
Figure 2. HBHDH ligand and its metal complexes have antifungal behavior
Conclusion
Various spectroscopy techniques, including analytical, infrared (IR), electrical, magnetic, and electronic absorption spectra, were used to validate the binding of ligands to metal. The research revealed that the HBHDH ligand exhibited a monobasic tridentate structure with transition metal ions. The Mn(II), Fe(II), Ni(II), Cu(II), and Co(II) complexes were determined to have octahedral geometry, whereas the Zn(II), Mn(II), Hg(II), and Cd(II)complexes were found to have square planar and tetrahedral geometry for Pd(II) complex. These conclusions were drawn from the findings of analytical and physicochemical examinations. The metal complexes exhibit a considerably greater antibacterial activity compared to the ligand. Typically, the ligand and its complexes have significantly greater efficacy against E. coli bacteria compared to normal Penicillin.
Conflict of interest
The authors declare that there is no conflict of interest regarding the publication of this paper.
Acknowledgements
Authors thank Dr. Chandrakant Jawale (Principal, Kai. Rasika Mahavidyalaya Deoni, Dist. Latur) for support, suggestions and encouragement during the execution of this project.
Orcid
Debraj Pallai: https://orcid.org/0009-0002-4879-9416
Raj Badekar: https://orcid.org/0000-0002-9425-4743
Ganpat R. Nagargoje: https://orcid.org/0009-0003-3796-8496
Abhay S. Bondge: https://orcid.org/0000-0002-6249-7185
Prasad D. Kadam: https://orcid.org/0009-0004-1480-2271
Kalimoddin I. Momin: https://orcid.org/0000-0001-6297-6509
Sharad P. Panchgalle: https://orcid.org/0000-0001-9706-7567
Vijaykumar S. More: https://orcid.org/0000-0002-6110-8774
Citation: D. Pallai, R. Badekar, K.I. Momin, A. Bondge, G.R. Nagargoje, P.D. Kadam, S.P. Panchgalle, V.S. More Synthesis, Spectral and Biological Studies of Co(II), Fe(II), Ni(II), Cu(II), Pd(II), Mn(II), Hg(II), Cd(II), and Zn(II) Complexes Derived from Benzohydrazide Schiff Base. J. Appl. Organomet. Chem., 2024, 4(1), 76-87.
----------------------------------------------------------------------------------------------------------------------------------------------------
OPEN ACCESS
©2024 The author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit: http://creativecommons.org/licenses/by/4.0/
PUBLISHER NOTE
Sami Publishing Company remains neutral concerning jurisdictional claims in published maps and institutional affiliations.
CURRENT PUBLISHER
Sami Publishing Company