Phytochemical Analysis of the Antioxidant Compounds of Baper Tea and Its Potential as an Immunomodulatory agent and Candidate for Standardized Herbal Medicine
DOI:
https://doi.org/10.48048/tis.2023.6391Keywords:
Baper tea, Covid-19, Immunomodulatory agents, Local wisdom, Standardized herbal medicineAbstract
The antioxidant content of Baper tea was determined using phytochemical testing, and the potential of these contents as immunomodulatory agents and candidates for standardized herbal medications was investigated. A randomized design was used in this experimental study. Phytochemical assays are used to determine the antioxidant content of Baper tea, whereas organoleptic studies are performed using hedonic tests. A literature review was used to assess the content's potential as an immunomodulatory agent and candidate for standardized herbal medicine. The study lasted four months at the Biology and National Food and Drug Agency Laboratory in Bali Province. Data analysis is based on descriptive methods. Baper tea is prepared in a 2.1.1 ratio. According to the hedonic test results, 41 panelists (87.23 %) prefer Baper tea products based on their color assessment, flavor, aroma, community reception, and acceptability. The matrix and security test revealed a water content of 4.43 %, an IC50 of 24.27 ppm, α-glucosidase inhibitors of 12.18 ppm, a total phenolic content of 28.00 %, and a total flavonoid content of 15.57 %. Baper tea raw materials contain components that have the potential to act as immunomodulatory agents and candidates for standardized herbal medications based on local wisdom.
HIGHLIGHTS
- There are very few standardized herbal medicinal products that are efficacious as immunomodulators in the market
- Shallot skin (Allium Cepa), morel berry leaves (Physalis angulata), and small gooseberry leaves (Phyllanthus urinaria) packaged in Baper Tea preparations contain bioactive compounds that have the potential to be used as immunomodulatory agents and candidate for standardized herbal medicine
- Baper tea contains very active antioxidant compounds at a formulation ratio of 2:1:1. With panelists' acceptance of Baper Tea reaching 87.23 %
GRAPHICAL ABSTRACT 
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E Burhan, F Isbaniah, AD Susanto , TY Aditama, Soedarsono, TR Sartono, YJ Sugiri, R Tantular, BYM Sinaga, RRD Handayani and H Agustin. COVID-19 Pneumonia: Diagnosis and Management in Indonesia. 1st ed. Indonesian Lung Doctors Association, Jakarta, Indonesia, 2020.
Gunadi, H Wibawa, MS Hakim, Marcellus, I Trisnawati, RE Khair, R Triasih, Irene, Afiahayati, K Iskandar, Siswanto, N Anggorowati, EW Daniwijaya, E Supriyati, DAA Nugrahaningsih, E Budiono, H Retnowulan, Y Puspadewi, I Puspitawati, O Sianipar, D Afandy, S Simanjaya, W Widitjiarso, DA Puspitarani, F Fahri, U Riawan, AR Fauzi, AS Kalim, NR Ananda, A Setyati, D Setyowireni, IS Laksanawati, E Arguni, T Nuryastuti and T Wibawa. Molecular epidemiology of SARS-CoV-2 isolated from COVID-19 family clusters. BMC Med. Genom. 2021; 14, 144.
R Nidom, N Foundation and S Indrasari. Viroinformatics investigation of B-cell epitope conserved region in SARS- CoV- 2 lineage B.1.1.7 isolates originated from Indonesia to develop vaccine candidate against COVID-19 Viroinformatics investigation of B-cell epitope conserved region in SAR. J. Pharm. Phcog. Res. 2021; 9, 766-79.
ANM Ansori, VD Kharisma, SS Muttaqin, Y Antonius and AA Parikesit. Genetic variant of SARS-CoV-2 isolates in Indonesia: Spike glycoprotein gene. J. Pure Appl. Microbiol. 2020; 14, 971-78.
HA Rothan and SN Byrareddy. The epidemiology and pathogenesis of coronavirus disease (COVID-19) outbreak. J. Autoimmun. 2020; 109, 102433.
E Parwanto. Response to mutation and variants of the SARS-CoV-2 gene. Universa Medicina 2021; 40, 77-8.
J Zhang, L Huang, C Zhang, S Liu, P Zhao, H Liu, L Zhu, Y Tai, C Bai, T Gao, J Song, P Xia, J Dong, J Zhao and W Fu-Sheng. Pathological findings of COVID-19 associated with acute respiratory distress syndrome. Lancet Respir. Med. 2020; 8, 420-22.
D Ridwanuloh and F Syarif. Isolation and identification of flavonoid compounds from ciplukan stem (Physalis angulata L.). Pharma Xplore 2019; 4, 288-96.
IR Aziz, D Armita, H Hajrah and K Makmur. Indonesian local plant regulatory genes: COVID-19 immunomodulators. Teknosains 2020; 14, 238-46.
LN Venu and A Austin. Antiviral efficacy of medicinal plants against respiratory viruses: Respiratory syncytial virus (RSV) and coronavirus (COV)/COVID 19. J. Phytopharmacology 2020; 9, 281-90.
IMOA Pawarta. Traditional medicine. 1st ed. Udayana University Press, Denpasar, Indonesia, 2017.
M Rus, DC Nistor-Cseppento, C Bustea, RAC Aron, C Pantis, G Zengin, A Sehgal, R Kaur, A Kumar, S Arora, D Setia, D Chandel and S Bungau. Exploring the multifocal role of phytochemicals as immunomodulators. Biomed. Pharmacother. 2021; 133, 110959.
V Handayani and N Nurfadillah. Pharmacognostic study of green meniran herb (Phyllanthus niruri L.) and red meniran herb (Phyllanthus urinaria L.). Indonesian J. Pharm. Nat. Prod. 2016; 1, 18-23.
IGAS Wangiyana, Supriadi, A Nikmatullah, et al. Phytochemical screening and antioxidant activity of Gyrinops tea from agarwood plantation on Lombok island, Indonesia. IOP Conf. Series Earth Environ. Sci. 2021; 712, 012029.
FP Luhurningtyas, J Susilo, R Yuswantina, E Widhihastuti and FW Ardiyansah. The immunomodulatory activity and phenolic content of red ginger rhizome extract (Zingiber officinale Rosc. Var. Rubrum). Indonesian J. Pharm. Nat. Prod. 2021; 4, 51-9.
N Marefati, N Eftekhar, M Kaveh, J Boskabadi, F Beheshti and MH Boskabady. The effect of allium cepa extract on lung oxidant, antioxidant, and immunological biomarkers in ovalbumin-sensitized rats. Med. Princ. Pract. 2018; 27, 122-28.
National Standardization Agency of Indonesia. SNI 3836: 2013 about packaged dried tea. National Standardization Agency of Indonesia, Jakarta, Indonesia 2013.
WHO. Guidelines on good herbal processing practices for herbal medicines, Fifty; expert committee on specifications for pharmaceutical preparations. WHO, Geneva, Switzerland, 2018.
M Darwin, MR Mamondol, SA Sormin, Y Nurhayati, H Tambunan, D Sylvia, IMDM Adnyana, B Prasetiyo, P Vianitati and AA Gebang. Quantitative approach research method. 1st ed. CV Media Sains Indonesia, Bandung, Indonesia, 2021.
DA Alfitasari, AM Kusuma and ZR Hakim. Immunodulatory activity of ethanol extract of shallots (allium cepa L.) on non-specific immune response in male mice of BALB/C strain by carbon clearance method. Biosfera 2017; 34, 75.
AV Anand, B Balamuralikrishnan, M Kaviya, K Bharathi, A Parithathvi, M Arun, N Senthilkumar, S Velayuthaprabhu, M Saradhadevi, NA Al-Dhabi, MV Arasu, MI Yatoo, R Tiwari and K Dhama. Medicinal plants, phytochemicals, and herbs to combat viral pathogens including sars-cov-2. Molecules 2021; 26, 1775.
L Febriana. Potential supplements in the management of COVID-19. Cont. Med. Educ. 2021; 48. 93-6.
V Mallajosyula, C Ganjavi, S Chakraborty, AM McSween, AJ Pavlovitch-Bedzyk, J Wilhelmy, A Nau, M Manohar, KC Nadeau and MM Davis. CD8+ T cells specific for conserved coronavirus epitopes correlate with milder disease in COVID-19 patients. Sci. Immunol. 2021; 6, eabg5669.
Ş Taştemur and H Ataseven. Quercetin in the treatment and prevention of COVID-19, COVID-19 tedavi ve profilaksisinde Quercetin. Cumhuriyet Med. J. 2021; 43, 100-16.
AV González-de-Peredo, M Vázquez-Espinosa, C Carrera, E Espada-Bellido, M Ferreiro-González, GF Barbero and M Palma. Development of a rapid UHPLC-PDA method for the simultaneous quantification of flavonol contents in onions (Allium cepa L.). Pharmaceuticals 2021; 14, 310.
B Putra, RN Azizah and EM Nopriyanti. Efek imunomodulator ekstrak etanol herba krokot (Portulaca oleracea L.) terhadap tikus putih (Rattus norvegicus) jantan dengan parameter delayed type hypersensitivity (DTH): Effect of immunomodulator of purslane herb ethanol (Portulaca oleracea L.) on Rat (Rattus norvegicus) with delayed type hypersensitivity (DTH) parameter. Galenika J. Pharm. 2020; 6, 20-5.
K Hyun-Na, H Seung-Heon, S Han-Geuk, Y Taek-Soo, L Ki-Nam, K Nam-Song, K Cheorl-Ho and K Hyung-Min. Inulin stimulates NO synthesis via activation of PKC-α and protein tyrosine kinase, resulting in the activation of NF-κB by IFN-γ-primed RAW 264.7 cells. J. Nutr. Biochem. 2013; 14, 598-605.
X Liu, J Xie, S Jia, L Huang, Z Wang, C Li and M Xie. Immunomodulatory effects of an acetylated cyclocarya paliurus polysaccharide on murine macrophages RAW264.7. Int. J. Biol. Macromol. 2017; 98, 576-81.
P Pandey, F Khan, A Kumar, A Srivastava and NK Jha. Screening of potent inhibitors against 2019 novel coronavirus (COVID-19) from allium sativum and allium cepa: An in silico approach. Biointerface Res. Appl. Chem. 2021; 11, 7981-93.
KH Darmawan. Utilization of nano ethanolic extract combination chamber bitter (Phyllanthus niruri L.) and Garlic (Allium sativum L.) as a natural immunomodulator in nanoherbal development, in silico and in vitro study. J. Pharmaceut. Sci. Clin. Res. 2017; 2,110.
L Sapra, A Bhardwaj, Z Azam, D Madhry, B Verma, S Rathore and RK Srivastava. Phytotherapy for treatment of cytokine storm in COVID-19. Front. Biosci. 2021; 26, 51-75.
S Khaerunnisa, H Kurniawan, R Awaluddin and S Suhartati. Potential inhibitor of COVID-19 main protease (M-pro) from several medicinal plant compounds by molecular docking study. Preprints 2020; 2020, 2020030226.
Y Octarina, E Prasetiyono, D Febrianti and R Robin. Effectiveness of ciplukan (Physalis angulata L.) leaf extract on immunity system of tilapia (Oreochromis niloticus). J. Riset Akuakultur 2018; 13. 259.
SR Rahayu and MW Diarti. Test the inhibition of ciplukan leaf filtrate (Physalis angulata linn) against the growth of Staphylococcus aureus bacteria. J. Anal. Med. Biosci. 2018, 5, 101-6.
H Febryantono, Siswanto, PE Santosa and M Hartono. The effects of meniran (Phyllanthus niruri L) extract dose on antibody titer of newcastle desease and avian influenza in male broiler.
Int. J. Anim. Husbandry Vet. Sci. 2020; 4, 52-8.
N Meiliana. Effect of oral administration of ethanol extract of dayak onion tubers (Eleuthrine palmifolia (L.) Merr) to BALB/c mice on prevention of decreased number of NK Cells and CD 8+. J. Biosci. 2016; 18, 13.
L Xia, Y Shi, J Su, T Friedemann, Z Tao, Y Lu, Y Ling, Y Lv, R Zhao, Z Geng, X Cui, H Lu and S Schröder. Shufeng Jiedu, a promising herbal therapy for moderate COVID-19: Antiviral and anti-inflammatory properties, pathways of bioactive compounds, and a clinical real-world pragmatic study. Phytomedicine 2020; 85, 153390.
T Rathinavel, S Thangaswamy, S Ammashi and S Kumarasamy. Virtual screening of COVID-19 drug from three indian traditional medicinal plants through in silico approach. Res. J. Biotechnol. 2020; 15, 124-40.
O Sytar, M Brestic, S Hajihashemi, M Skalicky, J Kubeš, L Lamilla-Tamayo, U Ibrahimova, S Ibadullayeva and M Landi. COVID-19 prophylaxis efforts based on natural antiviral plant extracts and their compounds. Molecul. 2021; 26, 727.
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