Metabolite Profile, Antioxidant Activity and Anti-Candida Activity of Fermented Star Fruit Bioextract (Averrhoa carambola L.)
DOI:
https://doi.org/10.48048/tis.2022.4183Keywords:
Metabolite profile, Antioxidant activity, Anti-candida activity, Fermented bioextract, Averrhoa carambola L.Abstract
Nowadays bioextracts are widely used in various applications, however there is limited information on chemical constituents and biological activities of the bioextract produced for consumption. Therefore, the purpose of this study was to investigate the antioxidant activity, anti-candida activity and metabolic profile of fermented star fruit (Averrhoa carambola L.) bioextract. The fermented star fruit bioextract was produced through fermentation lasting 180 days, during which the activity and the metabolite profile were determined at 30-day intervals. The antioxidant activity was tested using the DPPH radical scavenging assay and the anti-candida activity was determined by the broth micro-dilution method. The metabolic profile was analyzed using LC-ESI-QTOF-MS/MS. The results showed that as fermentation proceeded, the potency of antioxidant and anti-candida activity observed from the bioextract increased. The lowest IC50 value for DPPH inhibition (25 %v/v) and the lowest minimum inhibitory concentration (6.25 %v/v) against Candida albicans were both observed after fermentation for 150 days. The metabolite analysis identified 4 main chemical classes: Sugars, organic acids, flavonoids and nitrogen compounds. The chemical diversity was highest in the Day 120 through Day 150 samples. This study shows that fermented bioextract from star fruit at incubation time 150 - 180 days carries potential health benefits for application in anti-candida and antioxidant products. Possible toxicity, skin and oral mucosal irritation should be considered for further study.
HIGHLIGHTS
- The 150-day and 180-day star fruit bioextract exhibited strong anti-candida and antioxidant activities
- Four main chemical classes were found in the bioextract: Sugars, organic acids, flavonoids and nitrogen compounds
- The star fruit bioextract could be used as a source of anti-candida and antioxidant activity for development health promotion and cosmetic products
GRAPHICAL ABSTRACT
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P Dasgupta, P Chakraborty and NN Bala. Averrhoa carambola: An updated review. Int. J. Pharm. Res. Rev. 2013; 2, 54-63.
N Narain, PS Bora, HJ Holschuh and MADS Vasconcelos. Physical and chemical composition of carambola fruit (Averrhoa carambola L.) at three stages of maturity. Ciencia y Tecnologia Alimentaria 2001; 3, 144-8.
SAM Saghir, A Sadikun, K Kooi-Yeong and V Murugaiyah. Star fruit (Averrhoa carambola L.): From traditional uses to pharmacological activities. Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas 2013; 12, 209-19.
KB Silva, CTS Pinheiro, CRM Soares, MA Souza, TJ Matos-Rocha, SA Fonseca, JMSJ Pavão, JG Coata, LLS Pires and AF Santos. Phytochemical characterization, antioxidant potential and antimicrobial activity of Averrhoa carambola L. (Oxalidaceae) against multiresistant pathogens. Braz. J. Biol. 2021; 81, 509-15.
B Majhi, KB Satapathy and SK Mishra. Antimicrobial activity of Averrhoa carambola L. leaf extract and its phytochemical analysis. Res. J. Pharm. Tech. 2019; 12, 1219-24.
K Sripraya and N Trachoo. Antimicrobial activity of fermented fruit juices on selected foodborne pathogens. Asia Pac. J. Sci. Tech. 2008; 13, 906-18.
P Yasawardene, U Jayarajah, I De Zoysa and SL Seneviratne. Mechanisms of star fruit (Averrhoa carambola) toxicity: A mini-review. Toxicon 2020; 187, 198-202.
A Tancho. Applied nature farming: Principles, concepts and techniques in Thailand. National Science and Technology Development Agency, Pathumthani, Thailand, 2008.
Y Feng, M Zhang, AS Mujumdar and Z Gao. Recent research process of fermented plant extract: A review. Trends Food Sci. Tech. 2017; 65, 40-8.
S Phornphisutthimas. Fermented bio-extracts and agricultures. J. Res. Unit Sci. Tech. Environ. Learn. 2012; 3, 59-65.
N Kamla, V Limpinuntana, S Ruaysoongnern and RW Bell. Role of fermented bio-extracts produced by farmers on growth, yield and nutrient contents in cowpea (Vigna unguiculata (L.) Walp.) in Northeast Thailand. Biol. Agr. Horticulture 2008; 25, 353-68.
S Pimratch, B Toomsan and D Jothityangkoon. Wood vinegar and fermented bioextracts: Natural products to enhance growth and yield of tomato (Solanum lycopersicum L.). Scientia Horticulturae 2013; 154, 66-72.
C Noisopa, B Prapagdee, C Navanugraha and R Hutacharoen. Effects of Bio-extracts on the growth of Chinese kale. Agr. Nat. Resource 2010; 44, 808-15.
V Kunathigan and S Wiratthikowit. The application of “Bioextract” or “Bio-fermented solution” for treatment of domestic wastewater. In: Proceedings of the 2nd International Conference on Sustainable Global Agriculture and Food (ICSAF), Semarang, Indonesia. 2016, p. 51-61.
S Peerajan, C Chaiyasut, S Sirilun, K Chaiyasut, P Kesika and BS Sivamaruthi. Enrichment of nutritional value of Phyllanthus emblica fruit juice using the probiotic bacterium, Lactobacillus paracasei HII01 mediated fermentation. Food Sci. Tech. 2016; 36, 116-23.
C Chaiyasut. Fermented bioextracts. National Science and Technology Development Agency, Pathumthani, Thailand, 2010, p. 67-84.
C Liu, Y Xue, Y Ye, F Yuan, J Liu and J Shuang. Extraction and characterization of antioxidant compositions from fermented fruit juice of Morinda citrifolia (Noni). Agr. Sci. China 2007; 6, 1494-1501.
CY Wang, CC Ng, H Su, WS Tzeng and YT Shyu. Probiotic potential of noni juice fermented with lactic acid bacteria and bifidobacterial. Int. J. Food Sci. Nutr. 2009; 60, 98-106.
N Nuengchamnong, S Boonpathanasak and P Tepwitukkij. Rapid screening of antioxidant compounds in homemade fruit fermented juice using an online LC-ESI-MS/MS and DPPH assay. Chiang Mai J. Sci. 2011; 38, 430-8.
A Wojdyło, J Oszmiański and R Czemerys. Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chem. 2007; 105, 940-9.
W Brand-Williams, ME Cuvelier and C Berset. Use of a free radical method to evaluate antioxidant activity. LWT Food Sci. Tech. 1995; 28, 25-30.
LL Mensor, FS Menezes, GG Leitão, AS Reis, TC dos Santos, CS Coube and SG Leitão. Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother. Res. 2001; 15, 127-30.
MC Arendrup, J Meletiadis, JW Mouton, K Lagrou, P Hamal and J Guinea. EUCAST definitive document E. DEF 7.3. Method for the determination of broth dilution minimum inhibitory concentrations of antifungal agents for yeasts, Available at: https://www.eucast.org/fileadmin/src/
media/PDFs/EUCAST_files/AFST/Files/EUCAST_E_Def_7.3.2_Yeast_testing_definitive_revised_2020.pdf, accessed June 2020.
FP Valim, E Aguiar-Oliveira, ES Kamimura, VD Alves and RR Maldonado. Production of star fruit alcoholic fermented beverage. Indian J. Microbiol. 2016; 56, 476-81.
Y Lu, CW Tan, D Chen and SQ Liu. Potential of three probiotic lactobacilli in transforming star fruit juice into functional beverages. Food Sci. Nutr. 2018; 6, 2141-50.
M Liu, K Yang, Y Qi, J Zhang, M Fan and X Wei. Fermentation temperature and the phenolic and aroma profile of persimmon wine. J. Inst. Brewing 2018;124, 269-75.
N Sabokbar and F Khodaiyan. Total phenolic content and antioxidant activities of pomegranate juice and whey based novel beverage fermented by kefir grains. J. Food Sci. Tech. 2016; 53, 739-47.
S Nanasombat, K Khanha, J Phan-im, J Jitaied, S Wannasomboon, S Patradisakorn and A Wongsil. Antimicrobial and antioxidant activities of Thai local fruit extracts: Application of a selected fruit extract, Phyllanthus emblica linn. as a natural preservative in raw ground pork during refrigerated storage. Online J. Sci. Tech. 2012; 2, 1-7.
K Lakmal, P Yasawardene, U Jayarajah and SL Seneviratne. Nutritional and medicinal properties of Star fruit (Averrhoa carambola): A review. Food Sci. Nutr. 2021; 9, 1810-23.
G Shui and LP Leong. Residue from star fruit as valuable source for functional food ingredients and antioxidant nutraceuticals. Food Chem. 2006; 97, 277-84.
J Szutowska. Functional properties of lactic acid bacteria in fermented fruit and vegetable juices: A systematic literature review. Eur. Food Res. Tech. 2020; 246, 357-72.
OA Adebo and I Gabriela Medina-Meza. Impact of fermentation on the phenolic compounds and antioxidant activity of whole cereal grains: A mini review. Molecules 2020; 25, 927.
NS Ramadan, LA Wessjohann, A Mocan, DC Vodnar, NH El-Sayed, SA El-Toumy, DA Mohamed, ZA Aziz, A Enrlich and MA Farag. Nutrient and sensory metabolites profiling of Averrhoa carambola L. (Starfruit) in the context of its origin and ripening stage by GC/MS and chemometric analysis. Molecules 2020; 25, 2423.
BS Chidi, FF Bauer and D Rossouw. Organic acid metabolism and the impact of fermentation practices on wine acidity: A review. S. Afr. J. Enol. Viticulture 2018; 39, 1-15.
C Chaiyasut, S Jantavong, C Kruatama, P Sartjin, S Sirilun and L Shank. Factors affecting methanol content of fermented plant beverage containing Morinda citrifolia. Afr. J. Biotechnol. 2013; 12, 4356-63.
H Heymann and SE Ebeler. Rapid methods to analyze alcoholic beverages. In: H Heymann and SE Ebeler (Eds.). Sensory and instrumental evaluation of alcoholic beverages. Academic Press, San Diego, United States, 2016, p. 84-104.
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