Development of a Blocking ELISA Method for Detection of Getah Virus Antibodies in Horse Sera

Dong-Kun Yang; Yu-Ri Park; Ha-Hyun Kim; Hye Jeong Lee; Subin Oh; Bang-Hun Hyun

doi:10.4167/jbv.2023.53.2.170

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2023 Vol.53, Issue 2 Preview Page

Original Article

Development of a Blocking ELISA Method for Detection of Getah Virus Antibodies in Horse Sera

31 December 2023. pp. 170-178

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Abstract

Recent climate change and global warming are likely to increase mosquito-borne diseases. Getah virus (GETV) is a mosquito-borne virus that infects horses and other mammals, including humans. Currently, GETV infection in horse sera is confirmed by the virus neutralization (VN) test. Therefore, there is a need for a new enzyme-linked immunosorbent assay (ELISA) to detect GETV-antibody in a large number of horse serum samples. We aimed to develop a blocking ELISA (B-ELISA) method for the specific detection of GETV antibodies in horse serum samples. Antibodies against GETV in the sera of 175 horses were measured using the VN test. The purified QIAG9301-100P virus was used as an antigen for the B-ELISA. A monoclonal antibody (1E1) was conjugated with horseradish peroxidase and used as a detection antibody. For the establishment of the B-ELISA, antigen concentration, serum dilution factor, and conjugate dilution concentration were determined as 5 µg/mL, 20 times, and 3.6 µg/mL, respectively. We evaluated the sensitivity, specificity, and accuracy of the B-ELISA using sera from 175 horses. The B-ELISA had a diagnostic sensitivity of 91.4%, a specificity of 94.0%, and an accuracy of 93.1% compared with that of the VN test. The B-ELISA was significantly correlated with the VN test (r = 0.83). The new B-ELISA could replace the VN test and be useful for the sero-surveillance of GETV in horse sera. It is also expected to be able to detect GETV antibodies in the serum of various animals including pigs.

Keywords

Arboviruses

ELISA

Serology

Horse disease

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Information

Publisher :The Korean Society for Microbiology and The Korean Society of Virology
Publisher(Ko) :대한미생물학회‧대한바이러스학회
Journal Title :JOURNAL OF BACTERIOLOGY AND VIROLOGY
Volume : 53
No :2
Pages :170-178
Received Date : 2023-08-02
Revised Date : 2023-09-21
Accepted Date : 2023-10-17
DOI :https://doi.org/10.4167/jbv.2023.53.2.170

JOURNAL OF BACTERIOLOGY AND VIROLOGY ISSN:1598-2467(Print) 2093-0429(Online)

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