Plasma-Activated Electrospun Polylactide Fiber Meshes as Matrices for Tissue Engineering

Matthias Schnabelrauch; Ralf Wyrwa; Henrike Rebl; Claudia Bergemann; Birgit Finke; Michael Schlosser; Uwe Walschus; Silke Lucke; Klaus Dieter Weltmann; J. Barbara Nebe

doi:10.4028/www.scientific.net/MSF.783-786.1337

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HomeMaterials Science ForumMaterials Science Forum Vols. 783-786Plasma-Activated Electrospun Polylactide Fiber...

Plasma-Activated Electrospun Polylactide Fiber Meshes as Matrices for Tissue Engineering

Abstract:

Poly (L-lactide-co-D/L-lactide)-based fiber meshes resembling structural features of the native extracellular matrix have been prepared by electrospinning. Subsequent coating of the electrospun fibers with an ultrathin plasma polymerized allylamine (PPAAm) layer changed the hydrophobic nature of the polylactide surface into a hydrophilic polymer network and provided positively charged amino groups on the fiber surface able to interact with negatively charged pericellular matrix components. Cell experiments in vitro using different types of human epithelial cells (gingiva, uroepithel) revealed that the PPAAm-activated surfaces promoted the occupancy of the meshes by cells accompanied by improved initial cell spreading. An in vivo study in a rat intramuscular implantation model demonstrated that the local inflammatory tissue response did not differ between PPAAm-coated and untreated polylactide meshes.

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Periodical:

Materials Science Forum (Volumes 783-786)

Pages:

1337-1342

DOI:

https://doi.org/10.4028/www.scientific.net/MSF.783-786.1337

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Online since:

May 2014

Authors:

Matthias Schnabelrauch*, Ralf Wyrwa, Henrike Rebl, Claudia Bergemann, Birgit Finke, Michael Schlosser, Uwe Walschus, Silke Lucke, Klaus Dieter Weltmann, J. Barbara Nebe

Keywords:

Biodegradable Meshes, Cell Adhesion, Cell Matrices, Cytocompatibility, Electro-Spinning, In Vivo Study, Inflammation, Surface Coating, Tissue Regeneration

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References

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