Synthesis of Biopolymeric α-Lactalbumin Protein Nanoparticles and Nanospheres as Green Nanofluids Using in Drug Delivery and Food Technology

Pegah Esmaeilzadeh; Zahra Fakhroueian; Ali Akbar Miran Beigi

doi:10.4028/www.scientific.net/JNanoR.16.89

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Synthesis of Biopolymeric α-Lactalbumin Protein Nanoparticles and Nanospheres as Green Nanofluids Using in Drug Delivery and Food Technology
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HomeJournal of Nano ResearchJournal of Nano Research Vol. 16Synthesis of Biopolymeric α-Lactalbumin Protein...

Synthesis of Biopolymeric α-Lactalbumin Protein Nanoparticles and Nanospheres as Green Nanofluids Using in Drug Delivery and Food Technology

Abstract:

New nanofluids carrier containing fine homogeneous whey protein α-lactalbumin spherical nanoparticles and nanospheres have been synthesized by three methods in this research. Biochemical enzymatic catalysis by V8- protease, chemical hydrolysis (sol-gel assisted self assembly method) using cationic templates and metal ions ligand forming, and also by direct acidic titration method with applying various ethylene glycol derivatives containg alcohol solvents. Repeatability and stability of synthetic nanoparticles and nanospheres milk protein structures in clear and crystalline green water-based nanofluids was confirmed by dynamic light scattering (DLS) analysis and TEM images (2-5nm) even after a one year period of preparation. Fabrication of non-toxic nanosolutions-nanofluids including nonionic and cationic surfactants, cosurfactants and suitable solvents, besides proper incubation temperatures (37-50°C), adjusted aging times and doped of divalent ions–bridging (Ca2+, Mn2+ and Zn2+ ) between specific amino acids and carboxyl groups, are so vital factors in kinetically controlled self-assembly phenomenon of the natural protein building blocks to form high resistant and homogeneous nanoparticles and nanospheres in transparent and clear green nanofluids. Such nanofluids can have many applications in food and pharmaceutical industries for example in formulate foods with high nutritional value in oral and digestive (ingestion) routes, and drug delivery. Interesting textures of SEM images have also shown these new nanobiostructures of natural milk protein morphologies.

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Periodical:

Journal of Nano Research (Volume 16)

Pages:

89-96

DOI:

https://doi.org/10.4028/www.scientific.net/JNanoR.16.89

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Online since:

January 2012

Authors:

Pegah Esmaeilzadeh, Zahra Fakhroueian, Ali Akbar Miran Beigi

Keywords:

DSL Analysis, Green Nanofluids, Green Solvents, Polymer Protein Nanoparticles, Protein Nanospheres, Self-Assembly, Spherical Nanoparticles, α-Lactalbumin

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References

[1] Y. Huang, C.Y. Chiang, S.K. Lee, Y. Gao, E.L. Hu, J.D. Yoreo, A.M. Belcher, Programmable Assembly of Nanoarchitectures Using Genetically Engineered Viruses, Nano Lett. 5 (7) (2005) 1429-1434.

DOI: 10.1021/nl050795d

Google Scholar

[2] J.K. Vasir, M.K. Reddy, V.D. Labhasetwar, Nanosystems in drug targeting: opportunities and challenges, Current Nanosci. 1 (2005) 47-64.

DOI: 10.2174/1573413052953110

Google Scholar

[3] S. Pramanik, P. Banerjee, A. Sarkar, S.C. Bhattacharya, Size-dependent interaction of gold nanoparticles with transport protein: A spectroscopic study, J. Luminescence. 128 (2008) 1969-(1974).

DOI: 10.1016/j.jlumin.2008.06.008

Google Scholar

[4] C. Weber, C. Coester, J. Kreuter, K. Langer, Desolvation process and surface characterization of protein nanoparticles, Int. J. Pharm. 194 (1) (2000) 91-102.

DOI: 10.1016/s0378-5173(99)00370-1

Google Scholar

[5] L. Chen, G.E. Remondetto, M. Subirade, Food protein-based materials as nutraceutical delivery systems, Trends in Food Science & Technology 17 (2006) 272-283.

DOI: 10.1016/j.tifs.2005.12.011

Google Scholar

[6] R. Mehravar, M. Jahanshahi, N. Saghatoleslami, Production of biological nanoparticles from α-lactalbumin for drug delivery and food science application, Afr. J. Biotechnol. 8 (12) (2009) 6822-6827.

Google Scholar

[7] Q. Yuan, H.H. Duan, L.L. Li, L.D. Sun, Y.W. Zhang, C.H. Yan, Controlled synthesis and assembly of ceria-based nanomaterials, J. Colloid Interface Sci. 335 (2009) 151-167.

DOI: 10.1016/j.jcis.2009.04.007

Google Scholar

[8] J. Tang, F. Redl, Y. Zhu, T. Siegrist, L.E. Brus, M.L. Steigerwald, An organometallic synthesis of TiO2 nanoparticles, Nano Lett. 5 (2005) 543-548.

DOI: 10.1021/nl047992h

Google Scholar

[9] Y. Li, J. Zhou, S. Tung, E. Schneider, S. Xi, A review on development of nanofluid preparation and characterization, Powder Techno. 196 (2009) 89-101.

DOI: 10.1016/j.powtec.2009.07.025

Google Scholar

[10] L. Godson, B. Raja, D.M. Lal, S. Wongwises, Enhancement of heat transfer using nanofluids-An overview, Renewable and Sustainable Energy Reviews 14 (2010) 629-641.

DOI: 10.1016/j.rser.2009.10.004

Google Scholar

[11] B. Yang, Z.H. Han, Thermal conductivity enhancement in water-in-FC72 nanoemulsion fluids, Appl Phys. Lett. 88 (261914) (2006) 1-3.

DOI: 10.1063/1.2218325

Google Scholar

[12] D. Zhu, X. Li, N. Wang, X. Wang, J. Gao, H. Li, Dispersion behavior and thermal conductivity characteristics of Al2O3-H2O nanofluids, Curr. Appl. Phys. 9 (2009) 131-139.

DOI: 10.1016/j.cap.2007.12.008

Google Scholar