Microbial Community Composition on Lignite before and after the Addition of Phosphate Mining Wastes

Sören Bellenberg; Margarete Kalin; Wolfgang Sand

doi:10.4028/www.scientific.net/AMR.825.42

Paper Titles

Microbial Ecology in Extreme Acidic Pit Lakes from the Iberian Pyrite Belt (SW Spain)
p.23

Geochemistry and Microbiology in an Acidic, High Altitude (4,000 m) Salt Flat — High Andes, Northern Chile
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Quantification of the Microbial Community in Lateritic Deposits
p.33

Proteo and Actinobacteria Diversity at a Sulfide, Salt and Acid-Rich Lake in the North of Chile
p.37

Microbial Community Composition on Lignite before and after the Addition of Phosphate Mining Wastes
p.42

Underground Degradation of Lignite Coal Spoil Material by a Mixed Microbial Community under Acid Mine Drainage Conditions
p.46

Insights into Bacterial Community Structure of a Commercial Copper Bioheapleaching Plant: Growth of Heterotrophic Bacteria in the System
p.50

Enrichment of "Ferrovum" spp. and Gallionella Relatives Using Artificial Mine Water
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Comparison of Indigenous and Exogenous Mesophilic Bacteria for the Bioleaching of Low Grade Copper Ores
p.58

HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 825Microbial Community Composition on Lignite before...

Microbial Community Composition on Lignite before and after the Addition of Phosphate Mining Wastes

Abstract:

Empirical field tests in which phosphate mining wastes (NPR) were added to various sulphidic mining wastes resulted in neutral drainage. A biofilm was documented on waste rock covering the pyrite. This was likely the cause of the improved effluents. NPR was added to sterile columns containing pyritic lignite and spiked with granulated pyrite. The biological columns were inoculated with 10⁸ cells/g of lignite-cultured microbes from Rio Agrio, Argentina. The microbes were maintained at 30°C using pyrite as sole energy source. The microbial populations were monitored with MPN before NPR addition and 2, 4 and 10 weeks later. After 45 days strong bioleaching was evident, as measured by electrical conductivity. Concurrently, a shift in the microbial populations took place. In the presence of NPR about 90% of populations consisted of heterotrophic neutrophiles covering the pyrite surfaces with a 10 μm thick biofilm, whereas without NPR, 99% of the populations were iron oxidizing acidophiles in mono-layered biofilms. These results are relevant to controlling processes in heap leaching and acid rock drainage.

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Periodical:

Advanced Materials Research (Volume 825)

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42-45

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.825.42

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Online since:

October 2013

Authors:

Sören Bellenberg, Margarete Kalin, Wolfgang Sand

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