Evaluation of Crude Extracts of Mimosa pusica LINN. against Colletotrichum gloeosporioides to Control Anthracnose

Article Preview

Abstract:

The antifungal activity of crude extracts of M. pudica Linn. was evaluated by determining the minimum inhibitory concentration (MIC) of the solvent extracts. Subsequently, an antifungal assay was carried out by determining the inhibition of radial growth in PDA plates added with plant extracts. MIC value of crude extracts was 15.00, 18.50 and 19.50 µg/mL for 95% ethanol, dichloromethane and acetone extracts, respectively. Concerning the inhibition of radial growth, ethanol crude extracts of M. Pudica was most effective and exhibited highest antifungal activity of 55.65% at 10.00 µg/mL, 54.02% at 1.00 µg/mL, 50.95% at 0.10 µg/mL, and 48.70% at 0.01 µg/mL against C. gloeosporioides., respectively. The highest yield was found in 95% ethanol at 73 mg/g. The higher yield was presented when the higher polar solvents were used in this experiment. Since factors such as solvent of extraction may influence the properties of derived crude extract, different extraction methods may be of interest for further trial.

You might also be interested in these eBooks

Info:

Periodical:

Pages:

703-707

Citation:

Online since:

May 2013

Export:

Price:

[1] C.M. Garcia-Pajon and I.G. Collado: Natural Products Reports. Vol. 20 (2003), pp.426-431

Google Scholar

[2] A.M. Alvarez and W.T. Nishijima: Plant Disease. Vol. 71 (1987), pp.681-686

Google Scholar

[3] Jayakumar, R. Bhaskaran, S. Tsushina: Canadian Journal of Microbiology. Vol. 53 (2007), pp.196-206].

Google Scholar

[4] M.S. Mokbel and T. Suganuma: European Food Research and Technology. Vol. 224 (2004), pp.39-47

Google Scholar

[5] R.C. Hernazdez-Albiter, L.L. Barrera-Necha, S. Bautisata-Banos, L. Bravo-Luna: Revista Mexicana de Fitopatalogia. Vol. 25 (2007), pp.180-185

Google Scholar

[6] I. Somda, V. Leth, P. Sereme: Asian Journal of Plant Sciences. Vol. 6 (2007), pp.1182-1189

Google Scholar

[7] L.L. Barrera-Necha, S. Bautista-Banos, H.E. Flores-Montezuma, A. Rojas-Estudillo: Plant Pathology Journal. Vol. 7 (2008), pp.174-178

Google Scholar

[8] M. Naradisorn and A. Ruenkum: As. J. Food Ag-Ind. Special Issue (2009), p. S138-S142

Google Scholar

[9] L. Azmi, M. K. Singh and A.K. Akhtar: Int. J. Of Pharm.& Life Sci. Vol. 2 (11) (2011), pp.1226-1234

Google Scholar

[10] S. Peraza-Sánchez, E.O. Chan-Che, E. Ruiz-Sánchez: Journal of Agricultural Food Chemistry Vol. 53 (2005), p.2429–2432

Google Scholar

[11] V. Navarro, M.L. Villarreal, G. Rojas, X. Lozoya: Journal of Ethnopharmacology Vol. 53 (1996), p.143–147

Google Scholar

[12] Y. Ortiz-Núñez, I. Spengler-Salabarria, I. Collado, R. Hernandez-Galán: Journal of Agricultural Food Chemistry Vol. 54 (2006), p.7517–7521

Google Scholar

[13] L. Johnny, U. K. Yusuf, and R. Nulit: African Journal of Biotechnology Vol. 10 (2011), p.4157–4165

Google Scholar

[14] N. O. Ogbebor, A. T. Adekunle and D. A. Enobakhare: African Journal of Biotechnology Vol. 6(3) (2007), p.213–218

Google Scholar

[15] R. N. Okigbo and U. O. Ogbonnaya: African Journal of Biotechnology Vol. 5 (9) (2006), p.727– 731

Google Scholar

[16] S. Peraza-Sánchez, E. O. Chan-Che, E. Ruiz-Sánchez: Journal of Agricultural Food Chemistry, Vol. 53 (2005), p.2429– 2432

Google Scholar

[17] E. Guerrero-Rodríguez, S. Solís-Gaona, F.D. Hernández-Castillo, A. Flores-Olivas, V. Sandoval-López: Revista Mexicana de Fitopatología Vol. 25 (2007), p.48–53

Google Scholar