New Insights into Salt-Tolerance in Acidophilic Iron-Oxidising Bacteria

D. Barrie Johnson; Barry M. Grail; Violaine Bonnefoy

doi:10.4028/www.scientific.net/AMR.1130.3

Paper Titles

Foreword, Honouring Henry L. Ehrlich, Committees & Sponsors

New Insights into Salt-Tolerance in Acidophilic Iron-Oxidising Bacteria
p.3

Microbial Communities in Sediments in Acidic, Metal-Rich Mine Lakes: Results from a Study in South-West Spain
p.7

Influence of Different Growth Conditions on the Composition of Extracellular Polymeric Substances of Acidithiobacillus ferrooxidans and Acidithiobacillus ferrivorans Species
p.11

Comparative Genomics Underlines the Functional and Taxonomic Diversity of Novel “Ferrovum” Related Iron Oxidizing Bacteria
p.15

Screening and Isolation of Bacterial Strains with the Biotechnological Potential to Destruct Cyanide-Bearing Compounds under the Conditions of Extreme Continental Climate
p.19

Dyp-Type Peroxidase (DypA) from the Bioleaching Acidophilic Bacterium Leptospirillum ferriphilum DSM 14647
p.23

Global Transcriptional Responses of Acidithiobacillus ferrooxidans Wenelen under Different Sulfide Minerals
p.28

Comparison of Microbial and Geochemical Conditions of Lignite Coal Spoil and Overburden Areas and their Environmental Impact
p.32

HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 1130New Insights into Salt-Tolerance in Acidophilic...

New Insights into Salt-Tolerance in Acidophilic Iron-Oxidising Bacteria

Abstract:

Colonies of iron-oxidising acidophilic bacteria were isolated on solid media containing up to 500 mM NaCl from non-saline samples from the Rio Tinto (Spain). One of these isolates was identified as an "Acidithiobacillus ferriphilus" strain. Laboratory cultures of the type strain of Acidithiobacillus ferridurans grown on hydrogen for one year were also found to adapt to the presence of 500 mM salt. This culture also grew on sulfur, but not on ferrous iron, in media containing 500 mM NaCl. It regained its ability to oxidise iron only after protracted incubation in salt-free media. Molecular analysis found an insertion of about 1.5 kb in the regulatory region of the rus operon of the strain that was unable to oxidize iron. This insertion was lost in the strain that had subsequently regained this ability. Our results suggest that chloride tolerance and dissimilatory oxidation of iron by these bacteria do appear to be mutually exclusive.

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Periodical:

Advanced Materials Research (Volume 1130)

Pages:

3-6

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.1130.3

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Online since:

November 2015

Authors:

D. Barrie Johnson*, Barry M. Grail, Violaine Bonnefoy

Keywords:

Acidithiobacillus, Insertion Sequence, Iron Oxidation, Salt Tolerance

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