Study on Characteristics of Lactobacillus rhamnosus on Lactic Acid Fermentation with Different Substrates

Article Preview

Abstract:

In order to investigate characteristics of Lactobacillus rhamnosus on lactic acid fermentation using different substrate, in this study, saccharification liquid obtained during wet oxidation blasting of straw were used as substrate, conversion reducing sugar to lactate by Lactobacillus rhamnosus was studied and compared with fermentation using glucose as substrate, The results indicated that Lactobacillus rhamnosus could utilize reducing sugar in saccharification liquid obtained during wet oxidation blasting of straw effectively. The highest efficiency of reducing sugar conversion (92.45%) appeared when concentration of reducing sugar as substrate was 20mg/mL; conversion rate of reducing sugar decreased as increasing of sugar concentration. Fermentation of saccharification liquid obtained during wet oxidation blasting of straw to lactic acid by Lactobacillus rhamnosus had optimal effects in the first 48h of incubation.

You might also be interested in these eBooks

Info:

Periodical:

Pages:

388-392

Citation:

Online since:

October 2014

Export:

Price:

* - Corresponding Author

[1] S. Moraïs, Y. Barak, J. Caspi, Y. Hadar, R. Lamed, Y. Shoham, D.B. Wilson, E.A. Bayer, Cellulase-Xylanase Synergy in Designer Cellulosomes for Enhanced Degradation of a Complex Cellulosic Substrate, mBio. 1 (2010) 1-8.

DOI: 10.1128/mbio.00285-10

Google Scholar

[2] Y. Jiang, C. Xie, G. Yang, X. Gong, X. Chen, L. Xu, B. Bao, Cellulase-producing bacteria of Aeromonas are dominant and indigenous in the gut of Ctenopharyngodon idellus (Valenciennes), Aquac Res. 42 (2011) 499-505.

DOI: 10.1111/j.1365-2109.2010.02645.x

Google Scholar

[3] A. Singh, B. Tripathi, H. Sahay, R. Singh, R. Kaushik, A. Saxena, D. Arora, Biochemical and molecular characterization of thermo-alkali tolerant xylanase producing bacteria from thermal springs of Manikaran, Indian Journal of Microbiology. 50 (2010).

DOI: 10.1007/s12088-010-0071-4

Google Scholar

[4] S. Morais, Y. Barak, J. Caspi, Y. Hadar, R. Lamed, Y. Shoham, D.B. Wilson, E.A. Bayer, Contribution of a Xylan-Binding Module to the Degradation of a Complex Cellulosic Substrate by Designer Cellulosomes, Appl. Environ. Microbiol. 76 (2010).

DOI: 10.1128/aem.00266-10

Google Scholar

[5] A. Garde, G. Jonsson, A.S. Schmidt, B.K. Ahring, Lactic acid production from wheat straw hemicellulose hydrolysate by Lactobacillus pentosus and Lactobacillus brevis, Bioresource Technol. 81 (2002) 217-223.

DOI: 10.1016/s0960-8524(01)00135-3

Google Scholar

[6] G. Bustos, A.B. Moldes, J.M. Cruz, J.M. Domínguez, Production of fermentable media from vine-trimming wastes and bioconversion into lactic acid by Lactobacillus pentosus, J. Sci. Food Agr. 84 (2004) 2105-2112.

DOI: 10.1002/jsfa.1922

Google Scholar

[7] P. Alvira, E. Tomás-Pejó, M. Ballesteros, M.J. Negro, Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: A review, Bioresource Technol. 101 (2010) 4851-4861.

DOI: 10.1016/j.biortech.2009.11.093

Google Scholar

[8] W. Li, Z. Li, L. Wang, Y. Lü, H. Tian, D. Gong, Process of simultaneous saccharification and fermentation for rice straw after wet-oxidation explosion, Industrial Microbiology 41 (2011) 57-61.

Google Scholar

[9] L. Cheng, W. Mu, B. Jiang, Efficient Biotransformation of D-Galactose to D-Tagatose by Acidothermus Cellulolytics Atcc 43068, J. Food Biochem. 35 (2011) 1298-1310.

DOI: 10.1111/j.1745-4514.2010.00452.x

Google Scholar

[10] A.A. Bahar, K. Sezen, Z. Demirbağ, R. Nalçacioğlu, The relationship between insecticidal effects and chitinase activities of Coleopteran-originated entomopathogens and their chitinolytic profile, Ann. Microbiol. 62 (2011) 647-653.

DOI: 10.1007/s13213-011-0301-y

Google Scholar

[11] N. Liu, X.G. Chen, H.J. Park, C.G. Liu, C.S. Liu, X.H. Meng, L.J. Yu, Effect of MW and concentration of chitosan on antibacterial activity of Escherichia coli, Carbohyd, Polym. 64 (2006) 60-65.

DOI: 10.1016/j.carbpol.2005.10.028

Google Scholar

[12] H. Li, J. Lin, J. Lin, Y. Qu, X. Dong, Effects of high sugar concentration and nitrogen sources on L-lactic acid fermentation using Lactobacillus rhamnosus, Industrial Microbiology 2 (2006) 22-27.

Google Scholar