Can a low dosage of recombinant human bone morphogenetic protein-2 loaded on collagen sponge induce ectopic bone?

Dental Materials Journal

Online ISSN : 1881-1361
Print ISSN : 0287-4547
ISSN-L : 0287-4547

Original Paper

Can a low dosage of recombinant human bone morphogenetic protein-2 loaded on collagen sponge induce ectopic bone?

Mohammed Katib Alruwaili, Tsutomu SUGAYA, Yasuhito MORIMOTO, Ko NAKANISHI, Tsukasa AKASAKA, Yasuhiro YOSHIDA

Author information

Keywords: Recombinant human bone morphogenetic protein-2 (rhBMP-2), Absorbable collagen sponge (ACS), Bone formation, Osteoinduction

JOURNAL FREE ACCESS

2023 Volume 42 Issue 3 Pages 433-440

DOI https://doi.org/10.4012/dmj.2022-229

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Abstract

Recombinant human bone morphogenetic protein-2 (rhBMP-2) is one of the growth factors that may induce the formation of new bone. The aim was to determine the efficacy of low doses of rhBMP-2 for bone regeneration using a collagen sponge as a carrier. Three doses of rhBMP-2 (1.167, 0.117, and 0.039 mg/mL) were combined with an absorbable collagen sponge (ACS) as a delivery vehicle. The rhBMP-2/ACS implants were placed in the subcutaneous tissues of rat backs. X-ray microcomputed tomography (micro-CT) and histological analysis were used to evaluate bone formation. The samples treated with 1.167 mg/mL of rhBMP-2 showed greater bone formation than the samples treated with 0.117 mg/mL of rhBMP-2 four weeks after surgery. However, there was no evidence of bone formation in the samples that were treated with 0.039 mg/mL of rhBMP-2. It was found that rhBMP-2 was osteogenic even at one-tenth of its manufacturer’s recommended concentration (1.167 mg/mL), indicating its potential for clinical use at lower concentrations.

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© 2023 The Japanese Society for Dental Materials and Devices

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