Impact of nonalcoholic fatty liver disease on the risk of metachronous colorectal neoplasia after polypectomy

Nam Hee Kim; Yoon Suk Jung; Jung Ho Park; Dong Il Park; Chong Il Sohn

doi:10.3904/kjim.2019.360

Korean J Intern Med > Volume 36(3); 2021 > Article

Kim, Jung, Park, Park, and Sohn: Impact of nonalcoholic fatty liver disease on the risk of metachronous colorectal neoplasia after polypectomy

Original Article

Gastroenterology

Korean J Intern Med 2021;36(3):557-567.

Published online: July 7, 2020

DOI: https://doi.org/10.3904/kjim.2019.360

Impact of nonalcoholic fatty liver disease on the risk of metachronous colorectal neoplasia after polypectomy

Nam Hee Kim, Yoon Suk Jung

, Jung Ho Park, Dong Il Park, Chong Il Sohn

Division of Gastroenterology, Department of Internal Medicine, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea

Correspondence to Yoon Suk Jung, Ph.D. Division of Gastroenterology, Department of Internal Medicine, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, 29 Saemunan-ro, Jongno-gu, Seoul 03181, Korea Tel: +82-2-2001-8577 Fax: +82-2-2001-2049 E-mail: ys810.jung@samsung.com

Received October 29, 2019 Revised January 3, 2020 Accepted January 9, 2020

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background/Aims

Metabolic syndrome has been reported to be a risk factor for metachronous colorectal neoplasia (CRN). However, the impact of nonalcoholic fatty liver disease (NAFLD), a hepatic manifestation of metabolic syndrome, on the development of metachronous CRN after polypectomy has been rarely examined. We evaluated the association between NAFLD and the development of metachronous CRN after polypectomy.

Methods

Asymptomatic subjects who underwent abdominal ultrasonography and endoscopic removal of ≥ 1 adenomas at the index colonoscopy between 2010 and 2014, and had a follow-up surveillance colonoscopy until 2017 were analyzed.

Results

Of 6,182 participants, 2,642 (42.7%) had NAFLD at the time of the index colonoscopy. Patients with NAFLD had significantly higher cumulative incidence rates of metachronous overall CRN than those without NAFLD in both men (19.4% vs. 18.2% at 3 years and 49.2% vs. 44.0% at 5 years; p = 0.001) and women (18.7% vs. 10.5% at 3 years and 56.1% vs. 29.8% at 5 years; p < 0.001). Even after adjusting for confounders, NAFLD remained independently associated with an increased risk of metachronous overall CRN in both men (adjusted hazard ratio [HR], 1.17; 95% confidence interval [CI], 1.06 to 1.29) and women (adjusted HR, 1.63; 95% CI, 1.27 to 2.07). Additionally, NAFLD was an independent risk factor for metachronous advanced CRN (ACRN) in women (adjusted HR, 2.61; 95% CI, 1.27 to 5.37).

Conclusions

NAFLD is related to an increased risk of metachronous CRN after polypectomy. Especially, women with NAFLD are at an increased risk of developing metachronous ACRN. Our results indicate a possible effect of NAFLD on the pathogenesis of CRN.

Keywords: Non-alcoholic fatty liver disease; Colorectal neoplasms; Recurrence

INTRODUCTION

Colorectal cancer (CRC) is the third most commonly diagnosed cancer and the fourth leading cause of cancer-related deaths worldwide [1]. Screening colonoscopy with polypectomy is the most efficient strategy to reduce the incidence of CRC and its associated mortality [2]. Patients undergoing adenoma removal are recommended to undergo follow-up surveillance colonoscopy because of their higher risk of developing metachronous advanced adenomas or cancer [3-5]. The current guidelines on post-polypectomy surveillance stratify adenomas into two risk groups based on the baseline adenoma characteristics, and recommend follow-up surveillance colonoscopy every 5 to 10 years for patients with low-risk adenomas (1 to 2 tubular adenomas < 10 mm in size with low-grade dysplasia) and every 3 years for those with high-risk adenomas (≥ 3 adenomas or advanced adenomas) [3-5]. Recently, in addition to the baseline adenoma characteristics, some clinical features, such as the presence of metabolic syndrome, have also been established as important risk factors for the development of metachronous colorectal neoplasia (CRN) [6,7]. Identifying the risk factors for metachronous CRN can provide a more detailed and tailored approach to post-polypectomy surveillance for each individual patient.

Nonalcoholic fatty liver disease (NAFLD), a hepatic manifestation of metabolic syndrome, encompasses a histological spectrum ranging from simple steatosis to nonalcoholic steatohepatitis, and may subsequently progress to liver cirrhosis and hepatocellular carcinoma (HCC) [8]. However, the clinical burden of NAFLD is not confined to the liver [9]. NAFLD is also an emerging risk factor for type 2 diabetes, cardiovascular disease, and extrahepatic cancers, particularly CRC [10,11]. Several previous studies have reported a significant positive association between NAFLD and the prevalence of CRC [12,13]. In recent meta-analyses, the prevalence of colorectal adenomas (precursors of CRC) was also higher in patients with NAFLD [14,15]. Furthermore, the severity of NAFLD has been reported to be significantly correlated with the risk of CRN [16,17]. The results of previous studies have suggested that patients with NAFLD may also have an increased risk of developing metachronous CRN after polypectomy. However, there is a lack of data on the association between NAFLD and the risk of metachronous CRN development. Therefore, we aimed to compare the risk of metachronous CRN according to the presence or absence of NAFLD, and to identify whether NAFLD is associated with the development of metachronous CRN after polypectomy.

METHODS

Study population

The Kangbuk Samsung Health Study is a cohort study including South Korean men and women (aged ≥ 18 years) who underwent a comprehensive annual or biennial health examination at clinics associated with the Kangbuk Samsung Hospital Total Healthcare Center in Seoul and Suwon, Korea. The study population comprised a subset of the Kangbuk Samsung Health Study subjects who underwent screening colonoscopy as part of a comprehensive health examination between 2010 and 2017. We retrospectively analyzed the data obtained from a prospectively established cohort.

Patients who underwent abdominal ultrasonography (US) and endoscopic removal of ≥ 1 adenomas at the index colonoscopy between 2010 and 2014, and had a follow-up surveillance colonoscopy at an interval of ≥ 1 year until 2017, were considered to fulfill the inclusion criteria. All polyps were removed endoscopically at the index colonoscopy, and a single follow-up colonoscopy was performed in all patients. On the same day as the index colonoscopy, abdominal US was performed before the colonoscopy procedure.

In South Korea, the Industrial Safety and Health Law mandates free annual or biennial health screening examinations of all employees. Most of the participants of our study were employees of various companies and local governmental organizations, or their spouses. As part of their welfare policy, companies often subsidize comprehensive health examinations, including abdominal US and colonoscopy. In this study, general practitioners interviewed all participants before colonoscopy to ensure that they were asymptomatic (i.e., no abdominal pain, diarrhea, or hematochezia). Symptomatic participants were urged to seek medical care.

The exclusion criteria were as follows: a history of CRC or colorectal surgery, a history of inflammatory bowel disease, a diagnosis of CRC at the index colonoscopy, > 10 colorectal adenomas at the index colonoscopy, ≤ 1 year interval before the follow-up surveillance colonoscopy, age < 30 years, poor bowel preparation at the index or follow-up colonoscopy, excessive alcohol consumption (> 140 g/week for men and > 70 g/week for women), positivity to hepatitis B virus surface antigen, positivity to hepatitis C virus antibody, and a history of HCC. Poor bowel preparation was defined as “large amounts of solid fecal matter observed in the bowel precluding a satisfactory study, unacceptable preparation, or a condition in which < 90% of the mucosa could be adequately visualized” [18].

This study was approved by the Institutional Review Board of Kangbuk Samsung Hospital (approval no.2017-11-011, approved on November 7, 2017). The requirement for informed consent was waived because only de-identified data were retrospectively assessed.

Colonoscopic and histological examinations

Board-certified endoscopists performed all colonoscopic examinations using an EVIS LUCERA CV-260 colonoscope (Olympus Medical Systems, Tokyo, Japan). All detected polyps were removed through biopsy, polypectomy, or endoscopic mucosal resection, and the specimens were histologically assessed by experienced pathologists.

Overall CRN was defined as a cancer or any adenoma, and advanced CRN (ACRN) was defined as a cancer or advanced adenoma. Advanced adenoma was defined as the presence of one of the following features: ≥ 10 mm diameter, tubulovillous or villous structure, and high-grade dysplasia [3].

Measurements and definitions of variables

Data on health-related behaviors and medical history were obtained through a self-administered questionnaire, whereas physical measurements were obtained by trained staff. Smoking status was categorized as never, former, or current smoking. Family history of CRC was defined as the occurrence of CRC in ≥ 1 first-degree relatives at any age. Self-reported use of nonsteroidal anti-inflammatory drugs (NSAIDs) regularly over the month before study enrollment was recorded. The weekly frequency of moderate or vigorous physical activity was also assessed, and regular exercise was defined as exercising ≥ 3 times per week. Obesity was defined as a body mass index of ≥ 25 kg/m², which is the proposed cutoff value for diagnosing obesity in Asians [19].

The presence or absence of fatty liver was examined on abdominal US, which was performed using a 3.5-MHz transducer (LOGIQ 9; General Electric, Madison, WI, USA) by experienced radiologists who were unaware of the study aims. An ultrasonographic diagnosis of fatty liver was defined as the presence of a diffuse increase in fine echoes in the liver parenchyma compared with the kidney or spleen parenchyma [20]. For the diagnosis of fatty liver, the intraobserver agreement was extremely high (kappa 0.94) and the interobserver agreement was not low (kappa, 0.74) [21,22]. NAFLD was defined as the presence of fatty liver on abdominal US without other causes of chronic liver diseases as those described in the exclusion criteria.

Statistical analysis

Data are expressed as mean ± standard deviation or frequency (%). Baseline characteristics according to the presence or absence of NAFLD were compared using the chi-square test and Student’s t-test for categorical and continuous variables, respectively. The cumulative incidence of metachronous CRN according to the presence or absence of NAFLD was calculated using the Kaplan-Meier method, and the difference was determined using log-rank test. The hazard ratios (HRs) and corresponding 95% confidence intervals (CIs) for metachronous CRN were estimated using multivariable Cox proportional hazard regression analysis after adjusting for confounders including age, smoking status, family history of CRC, use of NSAIDs, obesity, regular exercise, and baseline adenoma characteristics. All reported p values were 2-tailed, and p < 0.05 was considered statistically significant. The software program SPSS version 21 (IBM Corp., Armonk, NY, USA) was used for statistical analyses.

RESULTS

Baseline characteristics of the study population

A total of 12,377 patients who underwent abdominal US and endoscopic removal of ≥ 1 adenomas at the time of the index colonoscopy, and had a follow-up surveillance colonoscopy, were eligible for inclusion in this study. Among them, 2,725 patients were excluded for the following reasons: a history of CRC or colorectal surgery (n = 97), a history of inflammatory bowel disease (n = 35), a diagnosis of CRC at the index colonoscopy (n = 9), >10 colorectal adenomas at the index colonoscopy (n = 3), ≤ 1 year interval before the follow-up colonoscopy (n = 278), age < 30 years (n = 131), and poor bowel preparation (n = 2,172). Additionally, 3470 patients with other causes of chronic liver diseases were excluded for the following reasons: excessive alcohol consumption (n = 3,098), positivity to hepatitis B virus surface antigen (n = 363), positivity to hepatitis C virus antibody (n = 8), and a history of HCC (n = 1). Finally, 6,182 patients were judged eligible for this study (Fig. 1).

Of the 6,182 eligible patients, 2,642 (42.7%) had NAFLD at the time of the index colonoscopy. The mean age of the study participants was 43.6 ± 8.4 years, and the proportion of men was 75.4%.

The baseline characteristics of the study participants are described in Table 1. Of the 4,659 male patients, 2331 (50.0%) had NAFLD at the time of the index colonoscopy. Among the men, the proportion of smokers and patients with obesity was higher in the NAFLD group than in the non-NAFLD group, whereas the proportion of patients with regular exercise was lower in the NAFLD group than in the non-NAFLD group. The mean interval between the index and follow-up colonoscopy was 3.5 ± 1.4 years, and the intervals were not significantly different between the two groups.

Of the 1,523 women, 311 (20.4%) had NAFLD at the time of index colonoscopy. Among the women, the mean age and the incidence of obesity were higher in patients with NAFLD than in those without NAFLD. The mean interval between the index and follow-up colonoscopy was 3.4 ± 1.4 years, and the intervals were longer in patients without NAFLD than in those with NAFLD.

Cumulative risk of metachronous CRN according to the presence or absence of NAFLD

Overall CRN developed in 1,999 (32.3%) patients and ACRN developed in 144 (2.3%) patients during a median follow-up of 3.3 years. The cumulative incidence rates of metachronous overall CRN on follow-up colonoscopy were compared between patients with and without NAFLD, separately in men and women (Fig. 2). In men, the 3- and 5-year cumulative incidence rates of metachronous overall CRN were significantly higher in patients with NAFLD than in those without NAFLD (19.4% vs. 18.2% and 49.2% vs. 44.0%, respectively; log-rank p = 0.001) (Fig. 2A). In women, the 3- and 5-year cumulative incidence rates of metachronous overall CRN were significantly higher in patients with NAFLD than in those without NAFLD (18.7% vs. 10.5% and 56.1% vs. 29.8%, respectively; log-rank p < 0.001) (Fig. 2B).

The 3- and 5-year cumulative incidence rates of metachronous ACRN were significantly higher in patients with NAFLD than in those without NAFLD among women (2.5% vs. 0.8% and 10.3% vs. 2.9%, respectively; log-rank p = 0.001) (Fig. 3B) but not among men (1.6% vs. 1.4% and 3.7% vs. 4.0%, respectively; log-rank p = 0.883) (Fig. 3A).

Risk factors associated with metachronous CRN

Table 2 shows the results of univariable and multivariable Cox regression analyses for the factors associated with the development of metachronous overall CRN. In the univariable analysis, NAFLD was associated with an increased risk of metachronous overall CRN development in both men (HR, 1.18; 95% CI, 1.07 to 1.29) and women (HR, 1.99; 95% CI, 1.59 to 2.50). Even after adjusting for potential confounders, NAFLD was still identified as an independent risk factor for metachronous overall CRN in both men (adjusted HR, 1.17; 95% CI, 1.06 to 1.29) and women (adjusted HR, 1.63; 95% CI, 1.27 to 2.07).

In addition, NAFLD was independently associated with an increased risk of metachronous ACRN in women (adjusted HR, 2.61; 95% CI, 1.27 to 5.37). However, there was no significant association between NAFLD and the risk of metachronous ACRN in men (Table 3).

We further performed a subgroup analysis according to menopausal status (Table 4). Of the 1,523 female patients, data on menopausal status were available in 1263 (82.9%). Of these, 863 patients (68.3%) were premenopausal and 400 patients (31.7%) were postmenopausal at the time of the index colonoscopy. NAFLD was associated with an increased risk of metachronous overall CRN in postmenopausal women (adjusted HR, 1.88; 95% CI, 1.32 to 2.67) but not in premenopausal women (adjusted HR, 1.37; 95% CI, 0.90 to 2.09). NAFLD was not significantly associated with the risk of metachronous ACRN in both premenopausal and postmenopausal women.

DISCUSSION

In this large-scale longitudinal study, we found that NAFLD was independently associated with an increased risk of the development of metachronous overall CRN after polypectomy in both men and women. Furthermore, NAFLD was an independent risk factor for metachronous ACRN in women. Our study provides clear evidence on the existence of an association between NAFLD and the development of CRN.

Accumulating epidemiological evidences have demonstrated that patients with NAFLD have a significantly higher prevalence of CRN than those without NAFLD [12-15]. NAFLD is considered a hepatic manifestation of metabolic syndrome [23,24], and is also associated with insulin resistance and a systemic low-grade inflammatory status [25]. NAFLD shares common risk factors with CRN, including central obesity, impaired glucose tolerance, and dyslipidemia [26,27]. The most likely potential mechanism linking NAFLD to the development of CRN is the proinflammatory and procarcinogenic effect of insulin resistance and the chronic inflammatory condition of patients with NAFLD [28,29]. Elevated serum levels of insulin and insulin-like growth factor may facilitate the development of CRN by promoting the proliferation of colonic epithelial cells and inhibiting apoptosis [27,29,30]. Proinflammatory cytokines, such as tumor necrosis factor-α, interleukin (IL)-6, IL-8, and plasminogen activator inhibitor-1, may also play important roles in colorectal carcinogenesis by promoting tumor cell proliferation and angiogenesis, and inhibiting apoptosis [31,32]. Additionally, decreased adiponectin levels and increased leptin levels in patients with NAFLD have procarcinogenic effects on the colorectum [33].

Several studies have reported a significant positive association between NAFLD and the prevalence of CRN [12-15], whereas data on the association between NAFLD and the risk of metachronous CRN at surveillance colonoscopy are extremely limited. To date, only two studies have attempted to investigate this topic [34,35]. One retrospective cohort study from Taiwan including 1,522 subjects reported that NAFLD was an independent risk factor for adenoma formation after a negative baseline colonoscopy (adjusted odds ratio [OR], 1.45; 95% CI, 1.07 to 1.98) [34]. However, the study only investigated the outcome of a specific time point. This is the reason why that study presented OR values from logistic regression analysis rather than HR values from Cox proportional hazards regression analysis. Additionally, in that study, only patients who had no adenoma at the index colonoscopy were included, and the interval between the initial (baseline) colonoscopy and the second colonoscopy (2.59 ± 1.24 years) was too short to analyze the risk of metachronous adenoma development in patients without adenoma at baseline. Another retrospective study including 441 NAFLD cases and 441 propensity score-matched controls demonstrated that NAFLD independently increased the risk of overall CRN occurrence with marginal significance (adjusted HR, 1.31; 95% CI, 1.01 to 1.71; p = 0.05) [35]. However, in a subgroup analysis based on index colonoscopy risk categories, the effect of NAFLD on the overall CRN occurrence at the time of surveillance colonoscopy was significant in the normal group (adjusted HR, 1.47; 95% CI, 1.05 to 2.06; p = 0.02), but not in the low- and high-risk groups. The negative results in the low- and high-risk groups might be because of the small number of patients in these groups. Among 441 subjects with NAFLD, only 85 and 38 were from the low- and high-risk groups, respectively, and among 441 subjects without NAFLD, only 100 and 34 were from the low- and high-risk groups, respectively. Our study with a relatively large sample size (5,176 patients with low-risk adenomas and 1,006 patients with high-risk adenomas) provides reliable evidence on the existence of a positive association between NAFLD and the risk of metachronous CRN in patients with adenoma at the index colonoscopy. Although our study did not include subjects with no adenoma at the index colonoscopy, according to the results of our study and the prior two studies, NAFLD seems to have an influence on metachronous CRN development regardless of the presence or absence of adenoma at the index colonoscopy.

Interestingly, we found that NAFLD was related to an increased risk of metachronous ACRN in women but not in men. This result suggests that NAFLD may have a stronger effect on colorectal carcinogenesis (especially the later stage of the adenoma-carcinoma sequence) in women than in men. The reasons for the sex-associated difference in the impact of NAFLD on the risk of metachronous ACRN development are unclear. Differences in sex hormones between men and women may be one of the reasons for the different results. To better assess the effects of sex hormones, we performed a subgroup analysis based on menopausal status. The results showed that NAFLD was associated with an increased risk of metachronous overall CRN in postmenopausal women but not in premenopausal women. These results may indicate that female hormones may have protective effects against metachronous CRN. However, NAFLD was not significantly associated with the risk of metachronous ACRN in postmenopausal women. This insignificant association may be because of the small number of postmenopausal women with metachronous ACRN (n = 7). Further studies are warranted to clarify the effects of NAFLD on metachronous CRN according to sex.

The current guidelines recommend the interval of post-polypectomy surveillance based solely on the baseline adenoma characteristics without considering the clinical risk factors for metachronous CRN [3-5]. Given that approximately one-third of the population has NAFLD and the incidence of this condition is markedly increasing [36,37], future risk stratification for surveillance colonoscopy may need to consider clinical risk factors such as NAFLD in addition to baseline adenoma characteristics. This might help provide a more detailed and tailored approach to each individual patient undergoing post-polypectomy surveillance.

To our knowledge, this is the first and largest longitudinal study demonstrating the impact of NAFLD on the risk of metachronous CRN development after adenoma removal. Nevertheless, the current study had several limitations. First, this was a hospital-based study rather than a population-based study and included only patients of Korean ethnicity. Moreover, most subjects were employees of various companies. Therefore, some degree of selection bias was likely present. Second, the possibility of inclusion of missed adenoma from index colonoscopy cannot be ruled out because of the retrospective nature of the study design. However, the likelihood of including missed adenoma is considered to be very low because our study selected and analyzed only high-quality data satisfying colonoscopy quality indicators, such as adenoma detection rate, withdrawal time of ≥ 6 minutes, and adequate bowel preparation. The overall adenoma detection rate in subjects aged ≥ 50 years at the index colonoscopy (between 2010 and 2014) was 32% (39% for men and 22% for women), which exceeded the recommended thresholds for colonoscopy quality (i.e., ≥ 25% overall or ≥ 30% for male patients and ≥ 20% for female patients) [38]. Third, the interval between the index and follow-up colonoscopy was relatively short (mean, 3.5 years). Accordingly, we did not include subjects without adenoma at the index colonoscopy. Fourth, fatty liver was diagnosed using abdominal US but was not confirmed with histological examination. However, liver biopsy is invasive and unfeasible in outpatients participating in health-checkup programs. Further, hepatic steatosis can be diagnosed using abdominal US with a high accuracy [39]. Lastly, the presence of fatty liver was evaluated only once (at the time of the index colonoscopy). The presence or severity of fatty liver may change over time. We did not assess the effect of dynamic changes of fatty liver status on the risk of metachronous CRN. However, during an average follow-up of 3.5 years, the presence or absence of fatty liver is likely to change in only a very small number of patients.

Despite these limitations, our data provide a better understanding of the association between NAFLD and the risk of metachronous CRN. NAFLD was identified as an independent risk factor for metachronous CRN development in both men and women, and was also an independent risk factor for metachronous ACRN in women. Our results indicate that NAFLD may have an impact on the risk of metachronous CRN development after polypectomy. Further large prospective studies are needed to clarify whether patients with NAFLD need more intensive surveillance after adenoma removal.

KEY MESSAGE

1. Nonalcoholic fatty liver disease (NAFLD) was independently associated with an increased risk of the development of metachronous overall colorectal neoplasia (CRN) after polypectomy in both men and women.

2. Furthermore, NAFLD was an independent risk factor for metachronous advanced CRN in women.

Conflict of Interest

No potential conflict of interest relevant to this article was reported.

Figure 1.

Flow diagram illustrating the selection of study subjects. NAFLD, nonalcoholic fatty liver disease.

Figure 2.

Cumulative incidence rate of metachronous overall colorectal neoplasia (CRN) according to the presence or absence of nonalcoholic fatty liver disease (NAFLD) in men (A) and women (B).

Figure 3.

Cumulative incidence rate of metachronous advanced colorectal neoplasia (ACRN) according to the presence or absence of nonalcoholic fatty liver disease (NAFLD) in men (A) and women (B).

Table 1.

Baseline characteristics according to nonalcoholic fatty liver disease status

Characteristic			Total (n = 6,182)	NAFLD (–) (n = 3,540)	NAFLD (+) (n = 2,642)	p value
Men			4,659	2,328	2,331
	Age, yr		43.3 ± 8.2	43.3 ± 8.5	43.3 ± 7.9	0.967
	Current or former smoking, %		63.7	62.0	65.5	0.015
	Family history of CRC, %		6.3	6.3	6.3	0.991
	Use of NSAIDs, %		3.2	3.0	3.4	0.364
	BMI, kg/m²		24.7 ± 2.9	23.4 ± 2.4	26.0 ± 2.7	< 0.001
	Obesity, BMI ≥ 25 kg/m², %		43.3	23.8	62.8	< 0.001
	Regular exercise^a, %		13.7	15.8	11.6	< 0.001
	Baseline adenoma characteristics					0.082
		Low-risk adenoma, %	83.9	84.9	83.0
		High-risk adenoma, %	16.1	15.1	17.0
	Interval between index and follow-up colonoscopy, yr		3.5 ± 1.4	3.5 ± 1.4	3.5 ± 1.4	0.762
Women			1,523	1,212	311
	Age, yr		44.7 ± 8.8	43.6 ± 8.3	48.9 ± 9.4	< 0.001
	Current or former smoking, %		4.5	4.4	5.1	0.559
	Family history of CRC, %		6.6	6.6	6.8	0.924
	Use of NSAIDs, %		4.8	4.7	5.1	0.745
	BMI, kg/m²		22.6 ± 3.2	21.8 ± 2.5	25.9 ± 3.5	< 0.001
	Obesity, BMI ≥ 25 kg/m², %		18.7	9.0	56.6	< 0.001
	Regular exercise^a, %		17.9	18.0	17.2	0.748
	Baseline adenoma characteristics					0.114
		Low-risk adenoma, %	83.1	83.8	80.1
		High-risk adenoma, %	16.9	16.2	19.9
	Interval between index and follow-up colonoscopy, yr		3.4 ± 1.4	3.5 ± 1.4	3.3 ± 1.3	0.027

Values are presented as mean ± standard deviation.

NAFLD, nonalcoholic fatty liver disease; CRC, colorectal cancer; NSAIDs, nonsteroidal anti-inflammatory drugs; BMI, body mass index.

^a Moderate or vigorous physical activity ≥ 3 times per week.

Table 2.

Risk factors for metachronous overall colorectal neoplasia

Variable	Crude HR (95% CI)		Adjusted HR (95% CI)^a
Variable	Men	Women	Men	Women
NAFLD
No	1 (reference)	1 (reference)	1 (reference)	1 (reference)
Yes	1.18 (1.07–1.29)	1.99 (1.59–2.50)	1.17 (1.06–1.29)	1.63 (1.27–2.07)
Age, yr	1.04 (1.03–1.04)	1.05 (1.04–1.06)	1.04 (1.03–1.04)	1.04 (1.03–1.05)
Current or former smoking	1.08 (0.98–1.19)	0.92 (0.56–1.51)	1.06 (0.96–1.18)	0.88 (0.53–1.45)
Family history of CRC	1.32 (1.09–1.59)	1.27 (0.86–1.87)	1.22 (1.01–1.48)	1.24 (0.83–1.85)
Use of NSAIDs	1.10 (0.85–1.42)	0.89 (0.54–1.47)	1.04 (0.80–1.35)	0.87 (0.52–1.46)
Regular exercise^b, %	1.03 (0.90–1.18)	1.07 (0.80–1.43)	0.94 (0.81–1.08)	0.99 (0.74–1.33)
Baseline adenoma characteristics
Low-risk adenoma	1 (reference)	1 (reference)	1 (reference)	1 (reference)
High-risk adenoma	1.57 (1.40–1.77)	1.58 (1.21–2.05)	1.40 (1.24–1.59)	1.28 (0.97–1.68)

HR, hazard ratio; CI, confidence interval; NAFLD, nonalcoholic fatty liver disease; CRC, colorectal cancer; NSAID, nonsteroidal anti-inflammatory drug.

^a Values were adjusted for NAFLD, age, current or former smoking, family history of CRC, use of NSAIDs, regular exercise, and baseline adenoma characteristics.

^b Moderate or vigorous physical activity ≥ 3 times per week.

Table 3.

Risk factors for metachronous advanced colorectal neoplasia

Variable	Crude HR (95% CI)		Adjusted HR (95% CI)^a
Variable	Men	Women	Men	Women
NAFLD
No	1 (reference)	1 (reference)	1 (reference)	1 (reference)
Yes	0.97 (0.67–1.42)	3.08 (1.56–6.07)	0.97 (0.66–1.42)	2.61 (1.27–5.37)
Age, yr	1.05 (1.03–1.07)	1.06 (1.03–1.10)	1.04 (1.02–1.67)	1.04 (1.01–1.08)
Current or former smoking	1.30 (0.86–1.96)	1.11 (0.27–4.64)	1.21 (0.80–1.83)	1.04 (0.24–4.58)
Family history of CRC	0.82 (0.33–2.01)	0.44 (0.06–3.20)	0.76 (0.31–1.88)	0.51 (0.07–3.72)
Use of NSAIDs	1.12 (0.41–3.05)	0.52 (0.07–3.79)	1.21 (0.44–3.29)	0.52 (0.07–3.81)
Regular exercise^b, %	1.29 (0.78–2.11)	1.45 (0.63–3.36)	1.10 (0.66–1.82)	1.45 (0.61–3.44)
Baseline adenoma characteristics
Low-risk adenoma	1 (reference)	1 (reference)	1 (reference)	1 (reference)
High-risk adenoma	3.51 (2.37–5.20)	4.20 (2.13–8.30)	2.96(1.97–4.45)	3.66 (1.80–7.45)

HR, hazard ratio; CI, confidence interval; NAFLD, nonalcoholic fatty liver disease; CRC, colorectal cancer; NSAID, nonsteroidal anti-inflammatory drug.

^a Values were adjusted for NAFLD, age, current or former smoking, family history of CRC, use of NSAIDs, regular exercise, and baseline adenoma characteristics.

^b Moderate or vigorous physical activity ≥ 3 times per week.

Table 4.

Subgroup analysis according to menopausal status

Variable	Metachronous overall CRN		Metachronous ACRN
Variable	Crude HR (95% CI)	Adjusted HR (95% CI)^a	Crude HR (95% CI)	Adjusted HR (95% CI)^a
Total women (n = 1,263)
NAFLD (−) (n = 987)	1 (reference)	1 (reference)	1 (reference)	1 (reference)
NAFLD (+) (n = 276)	1.97 (1.55–2.52)	1.65 (1.27–2.14)	2.66 (1.28–5.53)	2.25 (1.02–4.95)
Premenopausal women (n = 863)
NAFLD (−) (n = 720)	1 (reference)	1 (reference)	1 (reference)	1 (reference)
NAFLD (+) (n = 143)	1.37 (0.92–2.05)	1.37 (0.90–2.09)	2.55 (0.88–7.39)	2.90(0.93–9.07)
Postmenopausal women (n = 400)
NAFLD (−) (n = 267)	1 (reference)	1 (reference)	1 (reference)	1 (reference)
NAFLD (+) (n = 133)	2.04 (1.47–2.83)	1.88 (1.32–2.67)	2.32 (0.81–6.67)	1.67 (0.57–4.88)

Of the 1,523 female patients, 1,263 (82.9%) had available data on menopausal status.

CRN, colorectal neoplasia; ACRN, advanced colorectal neoplasia; HR, hazard ratio; CI, confidence interval; NAFLD, nonalcoholic fatty liver disease.

^a Values were adjusted for NAFLD, age, current or former smoking, family history of colorectal cancer, use of nonsteroidal anti-inflammatory drugs, regular exercise, and baseline adenoma characteristics.

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