DNA Barcoding Based Identification of Rosa x Damascene and Prunus dulcis Herbs Using ITS2 Barcoding Gene Amplification.
DOI:
https://doi.org/10.38211/joarps.2024.05.219Abstract
The considerable risk of adulteration in the herbs has raised commercial interest in the identification of medicinal herbs globally. DNA barcoding is the primary techniques for identifying the herbs at genetic level. This technique's key benefit is that it can identify the material's purity. This study focuses on the accurate identification of species utilizing Polymerase chain reaction-based nuclear universal internal transcribed spacer region (a barcode region) amplification and sequencing in 2 medically significant plants (Rosa damascene and Prunus dulcis) procured from the local herbal market of Karachi in the year 2022. Results suggest that the ribosomal nuclear ITS2 region of the selected plant species shows the 100 % identity with the reference genome, therefore it has shown a good rate of identification at the species level. The findings of this concludes that ITS2 is the novel standard barcode that involve in species identification, genomic conservation, and secure utilization of the medically significant plant species.
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Ahmed, S. S. (2022). DNA barcoding in plants and animals: a critical review. DOI: https://doi.org/10.20944/preprints202201.0310.v1
Aleksidze, N., Mchedlidze, K., Getia, M., Mulkijanyan, K., Frédérich, M., & Mskhiladze, L. (2021). Pharmacognostic, phytochemical and pharmacological aspects of Rosa x damascena Herrm. Georgian cultivar. GSC Biological and Pharmaceutical Sciences, 16(1), 001-010. DOI: https://doi.org/10.30574/gscbps.2021.16.1.0189
Barcodes, I. (2006). DNA barcoding evolves into the familiar. Conservation Biology, 20(5), 1548-1549. DOI: https://doi.org/10.1111/j.1523-1739.2006.00542.x
Chen, S., Yin, X., Han, J., Sun, W., Yao, H., Song, J., & Li, X. (2023). DNA barcoding in herbal medicine: Retrospective and prospective. Journal of Pharmaceutical Analysis. DOI: https://doi.org/10.1016/j.jpha.2023.03.008
de Boer, H. J., Ichim, M. C., & Newmaster, S. G. (2015). DNA barcoding and pharmacovigilance of herbal medicines. Drug safety, 38, 611-620. DOI: https://doi.org/10.1007/s40264-015-0306-8
Dev, S. A., Sijimol, K., Prathibha, P., Sreekumar, V., & Muralidharan, E. (2020). DNA barcoding as a valuable molecular tool for the certification of planting materials in bamboo. 3 Biotech, 10, 1-12. DOI: https://doi.org/10.1007/s13205-019-2018-8
Doyle, J. (1991). DNA protocols for plants Molecular techniques in taxonomy (pp. 283-293): Springer. DOI: https://doi.org/10.1007/978-3-642-83962-7_18
Gavra, D. I., Marian, E., Pallag, A., VICAȘ, L. G., Lucaciu, R. L., Micle, O., Sevastre, B. (2022). Phytochemical Screening and Biological Activity of Ethanolic Extract of Rosa X Damascena Mill. Cultivated In The Western Region of Romania. Farmacia, 70(2). DOI: https://doi.org/10.31925/farmacia.2022.2.9
Han, J., Pang, X., Liao, B., Yao, H., Song, J., & Chen, S. (2016). An authenticity survey of herbal medicines from markets in China using DNA barcoding. Scientific reports, 6(1), 18723. DOI: https://doi.org/10.1038/srep18723
Hassan, A. H. (2023). Dna Barcode Trnh-Psba Is A Promising Candidate Gene for Efficient Identification of Bitter And Sweet Almond And Related Species. Egyptian Journal of Desert Research, 73(1), 265-281. DOI: https://doi.org/10.21608/ejdr.2023.214645.1144
Keser, S., Demir, E., & Yılmaz, Ö. (2014). Phytochemicals and Antioxidant Activity of the Almond Kernel (Prunus dulcis Mill.) from Turkey. Journal of the Chemical Society of Pakistan, 36(3).
Matsuo, K. (2021). Evaluation of methods for plant genomic DNA sequence analysis without DNA and PCR product purification. Plant Science, 312, 111023. DOI: https://doi.org/10.1016/j.plantsci.2021.111023
Michel, C.-I., Meyer, R. S., Taveras, Y., & Molina, J. (2016). The nuclear internal transcribed spacer (ITS2) as a practical plant DNA barcode for herbal medicines. Journal of Applied Research on Medicinal and Aromatic Plants, 3(3), 94-100. DOI: https://doi.org/10.1016/j.jarmap.2016.02.002
Prgomet, I., Gonçalves, B., Domínguez-Perles, R., Pascual-Seva, N., & Barros, A. I. (2017). Valorization challenges to almond residues: Phytochemical composition and functional application. Molecules, 22(10), 1774. DOI: https://doi.org/10.3390/molecules22101774
Sahu, S. K., Thangaraj, M., & Kathiresan, K. (2012). DNA extraction protocol for plants with high levels of secondary metabolites and polysaccharides without using liquid nitrogen and phenol. International Scholarly Research Notices, 2012. DOI: https://doi.org/10.5402/2012/205049
Sayed, H. A., Mostafa, S., Haggag, I. M., & Hassan, N. A. (2023). DNA barcoding of prunus species collection conserved in the national gene bank of Egypt. Molecular Biotechnology, 65(3), 410-418. DOI: https://doi.org/10.1007/s12033-022-00530-z
Stoeckle, M. Y., Gamble, C. C., Kirpekar, R., Young, G., Ahmed, S., & Little, D. P. (2011). Commercial teas highlight plant DNA barcode identification successes and obstacles. Scientific reports, 1(1), 42. DOI: https://doi.org/10.1038/srep00042
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Copyright (c) 2024 Syed Rizwan, Hakim Abdul Bari, Arshad Saleem, Song Jingyuan, Xin Tianyi, Muhammad Jahanzeb
This work is licensed under a Creative Commons Attribution 4.0 International License.
