翻译后修饰蛋白质组学分离方法的研究进展

doi:10.3724/SP.J.1123.2016.08047

色谱 ›› 2016, Vol. 34 ›› Issue (12): 1199-1206.DOI: 10.3724/SP.J.1123.2016.08047

翻译后修饰蛋白质组学分离方法的研究进展

许静静^1,2, 刘幸¹, 周虎¹

1. 中国科学院上海药物研究所分析化学研究室, 上海 201203;
2. 上海市食品药品检验所化学药品室, 上海 201203

收稿日期:2016-08-31 出版日期:2016-12-08 发布日期:2013-04-24
通讯作者: 周虎,E-mail:zhouhu@simm.ac.cn
基金资助:
国家自然科学基金（21375138）；中国科学院“百人计划”；国家自然科学基金青-科学基金项目（31500110）；江苏省儿童呼吸疾病（中医药）重点实验室开放研究课题（JKLPRD201404）。.

Recent advances in separation methods for post-translational modification proteomics

XU Jingjing^1,2, LIU Xing¹, ZHOU Hu¹

1. Department of Analytical Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China;
2. Chemical Drugs Department, Shanghai Institute of Food and Drug Control, Shanghai 201203, China

Received:2016-08-31 Online:2016-12-08 Published:2013-04-24
Supported by:
National Natural Science Foundation of China (No. 21375138); "One Hundred Talented People" of the Chinese Academy of Sciences; Youth Science Fund Project of National Natural Science Foundation of China (No. 31500110); Jiangsu Province Key Laboratory of Children's Respiratory Diseases (Traditional Chinese Medicine) Open Research Topic (No. JKLPRD201404).

摘要/Abstract

摘要：

蛋白质翻译后修饰（PTMs）是调节细胞内生理活动的重要途径。该文总结了近年来PTMs蛋白质组学相关的分离方法，包括反相（RP）色谱法、离子交换（IEX）色谱法、亲水相互作用色谱（HILIC）法、多孔石墨化碳（PGC）色谱法、毛细管电泳（CE）法及分子筛色谱（SEC）法等。这些新方法为磷酸化、乙酰化、糖基化等PTM肽段或蛋白质的鉴定提供了更高的分离度和灵敏度。此外，该文也介绍了蛋白质领域其他重要分离方法的研究进展，这些方法可能被进一步应用于PTMs蛋白质组学的研究中。

关键词: 蛋白质组学, 多孔石墨化碳色谱, 翻译后修饰, 反相色谱, 离子交换色谱, 毛细管电泳, 亲水相互作用色谱

Abstract:

Post-translational modifications (PTMs) are the key control of many cellular processes. Current progresses of separation methods for PTMs proteomics, including reversed phase (RP) chromatography, ion exchange (IEX) chromatography, hydrophilic interaction chromatography (HILIC), porous graphitic carbon (PGC) chromatography, capillary electrophoresis (CE) and size-exclusion chromatography (SEC) are reviewed. The methods for the identification of PTMs have higher resolution and sensitivity. In addition, some other important separation methods for proteomics study are introduced, and they may be applied in the study of PTMs with further optimization in the future.

Key words: capillary electrophoresis (CE), hydrophilic interaction chromatography (HILIC), ion exchange (IEX) chromatography, porous graphitic carbon (PGC) chromatography, post-translational modifications (PTMs), proteomics, reversed phase (RP) chromatography

中图分类号:

O658

许静静, 刘幸, 周虎. 翻译后修饰蛋白质组学分离方法的研究进展[J]. 色谱, 2016, 34(12): 1199-1206.

XU Jingjing, LIU Xing, ZHOU Hu. Recent advances in separation methods for post-translational modification proteomics[J]. Chinese Journal of Chromatography, 2016, 34(12): 1199-1206.

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翻译后修饰蛋白质组学分离方法的研究进展

Recent advances in separation methods for post-translational modification proteomics

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