Network of miR396-mRNA in Tissue Differentiation in Moso Bamboo (Phyllostachys edulis)
Abstract
:1. Introduction
2. Results
2.1. A Total of 1128 Predicted Target Genes of Five miR396 Members
2.2. Three Up-Regulated miR396s in Bamboo Underground Shoots
2.3. Four Differentially Expressed miR396s in 13 Combination Pairs
2.4. Ten Confirmed MiRNA-mRNA Pairs by Degradome Sequencing
2.5. MiR396s Specifically Bind to PeGRF TF Genes and Are Localized in Vascular Tissues
3. Discussion
3.1. Bamboo miR396-mRNA Regulatory Module in Vascular Tissues
3.2. Potential Role of Bamboo miR396 in Vascular Tissue Differentiation
3.3. Potential Role of miR396 in Xylem Patterning
4. Materials and Methods
4.1. Plant Materials
4.2. Data Collection
4.3. MiR396 Identification and Annotation
4.4. Analysis of miR396 Expression
4.5. Target Prediction and Annotation
4.6. Expression Analysis of the Predicted Targets
4.7. Degradome Analysis of miR396-mRNA Interaction Pairs
4.8. Sequence Analysis and Phylogenetic Tree Construction
4.9. Dual-Luciferase Reporter Assays in N. benthamiana Leaves
4.10. RNA FISH
4.11. Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Li, Y.; Vasupalli, N.; Cai, O.; Lin, X.; Wu, H. Network of miR396-mRNA in Tissue Differentiation in Moso Bamboo (Phyllostachys edulis). Plants 2023, 12, 1103. https://doi.org/10.3390/plants12051103
Li Y, Vasupalli N, Cai O, Lin X, Wu H. Network of miR396-mRNA in Tissue Differentiation in Moso Bamboo (Phyllostachys edulis). Plants. 2023; 12(5):1103. https://doi.org/10.3390/plants12051103
Chicago/Turabian StyleLi, Ying, Naresh Vasupalli, Ou Cai, Xiaofang Lin, and Hongyu Wu. 2023. "Network of miR396-mRNA in Tissue Differentiation in Moso Bamboo (Phyllostachys edulis)" Plants 12, no. 5: 1103. https://doi.org/10.3390/plants12051103