Differential Impact of CD43 and CD28 on T-Cell Differentiation Depending on the Order of Engagement with the TCR
Abstract
:1. Introduction
2. Results
2.1. Engaging either CD28 or CD43 with the TCR Results in Robust T-Cell Activation
2.2. Engagement of CD43 or CD28 before or after TCR Activation Leads to Different Outcomes
2.3. The Timing of CD28 or CD43 Engagement Relative to the TCR Defines Distinct Activation Patterns
2.4. Different Activation Programs Predict the Usage of Specific Groups of Transcription Regulatory Elements and Different Outcomes
3. Discussion
4. Materials and Methods
4.1. Cell Activation
4.2. Flow Cytometry
4.3. Cytokine Detection
4.4. Image Analysis and Data Normalization
4.5. Correlations, Principal Component Analysis, and Protein Expression Signatures
4.6. Differential Protein Expression Analysis
4.7. Transcription Factors Prediction
4.8. Ingenuity Pathway Analysis
4.9. Clustering Analysis
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sandoval-Hernández, M.A.; Fierro, N.A.; Veytia-Bucheli, J.I.; Alvarado-Velázquez, D.A.; Alemán-Navarro, E.; Melchy-Pérez, E.; Auvynet, C.; Imaz-Rosshandler, I.; Carneiro, J.; Perez-Rueda, E.; et al. Differential Impact of CD43 and CD28 on T-Cell Differentiation Depending on the Order of Engagement with the TCR. Int. J. Mol. Sci. 2024, 25, 3135. https://doi.org/10.3390/ijms25063135
Sandoval-Hernández MA, Fierro NA, Veytia-Bucheli JI, Alvarado-Velázquez DA, Alemán-Navarro E, Melchy-Pérez E, Auvynet C, Imaz-Rosshandler I, Carneiro J, Perez-Rueda E, et al. Differential Impact of CD43 and CD28 on T-Cell Differentiation Depending on the Order of Engagement with the TCR. International Journal of Molecular Sciences. 2024; 25(6):3135. https://doi.org/10.3390/ijms25063135
Chicago/Turabian StyleSandoval-Hernández, Monserrat Alba, Nora Alma Fierro, José Ignacio Veytia-Bucheli, Den Alejandro Alvarado-Velázquez, Estefanía Alemán-Navarro, Erika Melchy-Pérez, Constance Auvynet, Iván Imaz-Rosshandler, Jorge Carneiro, Ernesto Perez-Rueda, and et al. 2024. "Differential Impact of CD43 and CD28 on T-Cell Differentiation Depending on the Order of Engagement with the TCR" International Journal of Molecular Sciences 25, no. 6: 3135. https://doi.org/10.3390/ijms25063135