Neural Stem Cells Transplanted into Rhesus Monkey Cortical Traumatic Brain Injury Can Survive and Differentiate into Neurons
Abstract
:1. Introduction
2. Results
2.1. Induction and Identification of NSCs
2.2. Survival and Differentiation of Grafted NSCs in the Site of TBI One Year after Transplantation
2.3. Activity and Migration of the Grafted NSCs
2.4. Differentiation of Grafted NSCs into Astrocytes
2.5. Differentiation of Grafted NSCs into Dopaminergic Neurons
3. Discussion
4. Materials and Methods
4.1. Embryonic Stem Cell Culture
4.2. Induction of NSC Differentiation
4.3. Induction of Dopaminergic Neurons Differentiation
4.4. Immunofluorescence Analysis of NSCs
4.5. Western Blot Analysis of NSCs
4.6. Brain Damage Surgery
4.7. Transplantation of NSCs
4.8. Toluidine Blue Staining
4.9. Identification of Cells by Immunofluorescence
4.10. Statistics
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Liu, S.; Shi, L.; Huang, T.; Luo, Y.; Chen, Y.; Li, S.; Wang, Z. Neural Stem Cells Transplanted into Rhesus Monkey Cortical Traumatic Brain Injury Can Survive and Differentiate into Neurons. Int. J. Mol. Sci. 2024, 25, 1642. https://doi.org/10.3390/ijms25031642
Liu S, Shi L, Huang T, Luo Y, Chen Y, Li S, Wang Z. Neural Stem Cells Transplanted into Rhesus Monkey Cortical Traumatic Brain Injury Can Survive and Differentiate into Neurons. International Journal of Molecular Sciences. 2024; 25(3):1642. https://doi.org/10.3390/ijms25031642
Chicago/Turabian StyleLiu, Shuyi, Liping Shi, Tianzhuang Huang, Yuyi Luo, Yongchang Chen, Shangang Li, and Zhengbo Wang. 2024. "Neural Stem Cells Transplanted into Rhesus Monkey Cortical Traumatic Brain Injury Can Survive and Differentiate into Neurons" International Journal of Molecular Sciences 25, no. 3: 1642. https://doi.org/10.3390/ijms25031642