mRNA Levels of Aromatase, 5α-Reductase Isozymes, and Prostate Cancer-Related Genes in Plucked Hair from Young Men with Androgenic Alopecia
Abstract
:1. Introduction
2. Results
2.1. 5α-R Isozymes and Aromatase mRNA Levels
2.2. Analysis of Gene Expression Profiles via PCR Array
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Subjects and Sampling
5.2. RNA Isolation
5.3. Reverse Transcription and Quantitative Real-Time PCR
5.4. Human PCa PCR Array
5.5. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Symbol | Description | Fold Changes | p |
---|---|---|---|
APC | Adenomatous polyposis coli | 3.62 | 0.001070 |
AR | Androgen receptor | 4.87 | 0.047702 |
ETV1 | Ets variant 1 | 2.18 | 0.028468 |
IL6 | Interleukin 6 | 4.35 | 0.019576 |
FOXO1 | Forkhead box O1 | −4.00 | 0.009073 |
GPX3 | Glutathione peroxidase 3 | −2.56 | 0.035205 |
Differentially Methylated Promoters | APC, AR, CAV1, CCNA1, CDH1 (E-Cadherin), CDKN2A (P16INK4A), DKK3, DLC1, EDNRB, GPX3, GSTP1, MGMT (AGT), MSX1, PDLIM4 (RIL), PTGS2 (COX2), RARB, RASSF1, SFRP1, SLC5A8, TIMP2, TNFRSF10D, ZNF185. |
Upregulated in Prostate Cancer | ARNTL(BMAL1), CAMSAP1, DDX11, ECT2, ETV1, HAL, IGFBP5, KLK3, MTO1, PDPK1, RBM39, SOCS3, SOX4, SUPT7L. |
Downregulated in Prostate Cancer | CCND2, CLN3, GCA, IGF1, LGALS4, LOXL1, PPP2R1B, SFRP1, SLC5A8, TFPI2, USP5. |
Metastatic Potential | CREB1, KLHL13, MAX, NDRG3, PES1, SCAF11, SEPT7. |
Androgen Receptor Signaling | AR, CAV1, CCND1, DAXX, EGFR(ERBB1), FOXO1, GNRH1, IGF1, IL6, NFKB1, NRIP1, PTEN, SHBG, TGFB1I1, TIMP2, TIMP3, VEGFA. |
AKT & PI3 Kinase Signaling | AKT1, AR, BCL2, CCND1, CCND2, CDH1 (E-Cadherin), CDKN2A (P16INK4A), EGFR(ERBB1), FOXO1, GNRH1, IGF1, IL6, MAPK1 (ERK2), NFKB1, PDPK1, PTEN, TIMP2, TIMP3, TNFRSF10D, TP53 (p53), VEGFA. |
PTEN Signaling | AKT1, EGFR (ERBB1), GNRH1, IGF1, IL6, MAPK1 (ERK2), PDPK1, PTEN, TIMP2, TIMP3, TP53 (p53), VEGFA. |
Apoptosis | BCL2, CASP3, CDKN2A (P16INK4A), EGFR (ERBB1), ETV1, GNRH1, IGF1, IL6, MAPK1 (ERK2), NFKB1, PTEN, TIMP2, TIMP3, TP53 (p53), VEGFA. |
Cell Cycle | APC, BCL2, CASP3, CAV2, CCNA1, CCND1, CCND2, CDKN2A (P16INK4A), EGFR (ERBB1), IGF1, PPP2R1B, PTEN, PTGS1 (COX1), PTGS2 (COX2), TP53 (p53). |
Transcription Factors | AR, ARNTL (BMAL1), CDKN2A (P16INK4A), CREB1, DAXX, EGR3, ERG, ETV1, FOXO1, MAX, MSX1, NFKB1, NKX3-1, NRIP1, RARB, RBM39, SOX4, SREBF1, SUPT7L, TP53 (p53). |
Fatty Acid Metabolism | ACACA, CAMKK1, FASN, HMGCR, IGF1, PRKAB1, SREBF1, STK11 (LKB1). |
Other Prostate Cancer Genes | MKI67, TMPRSS2. |
Primer | Forward | Reverse | Size | Annealing Tª (°C) |
---|---|---|---|---|
SRD5A1 | AGCCATTGTGCAGTGTATGC | AGCCTCCCCTTGGTATTTTG | 136 | 55 |
SRD5A2 | TGAATACCCTGATGGGTGG | CAAGCCACCTTGTGGAATC | 154 | 54 |
SRD5A3 | TCCTTCTTTGCCCAAACATC | CTGATGCTCTCCCTTTACGC | 212 | 60 |
CYP19A1 | TATTAGGGCCCTGTGTCTGC | TGGGTTGGGACTTTTCCTCC | 193 | 60 |
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Sánchez, P.; Serrano Falcón, C.; Martínez Rodríguez, S.; Torres, J.M.; Serrano, S.; Ortega, E. mRNA Levels of Aromatase, 5α-Reductase Isozymes, and Prostate Cancer-Related Genes in Plucked Hair from Young Men with Androgenic Alopecia. Int. J. Mol. Sci. 2023, 24, 17461. https://doi.org/10.3390/ijms242417461
Sánchez P, Serrano Falcón C, Martínez Rodríguez S, Torres JM, Serrano S, Ortega E. mRNA Levels of Aromatase, 5α-Reductase Isozymes, and Prostate Cancer-Related Genes in Plucked Hair from Young Men with Androgenic Alopecia. International Journal of Molecular Sciences. 2023; 24(24):17461. https://doi.org/10.3390/ijms242417461
Chicago/Turabian StyleSánchez, Pilar, Cristina Serrano Falcón, Sergio Martínez Rodríguez, Jesús M. Torres, Salvio Serrano, and Esperanza Ortega. 2023. "mRNA Levels of Aromatase, 5α-Reductase Isozymes, and Prostate Cancer-Related Genes in Plucked Hair from Young Men with Androgenic Alopecia" International Journal of Molecular Sciences 24, no. 24: 17461. https://doi.org/10.3390/ijms242417461