Therapeutic Potential of Adipose-Derived Stem Cell-Conditioned Medium and Extracellular Vesicles in an In Vitro Radiation-Induced Skin Injury Model
Abstract
:1. Introduction
2. Results
2.1. Characterization of ADSCs and ADSC-EVs
2.2. Construction of the In Vitro Radiation-Induced Skin Injury Model
2.3. Effects of ADSC-CM and ADSC-EVs on HDFs
2.4. ADSC-CM and ADSC-EVs Promoted Collagen Synthesis of HDFs
2.5. Gene Expression Differences in Samples Treated with ADSC-CM or ADSC-EVs
3. Discussion
4. Materials and Methods
4.1. Patients
4.2. Isolation and Culture of ADSCs and HDFs
4.3. Preparation of ADSC-CM
4.4. Isolation and Collection of ADSC-EVs
4.5. Flow Cytometry
4.6. Western Blotting
4.7. Radiation Conditions and Evaluation of the In Vitro Model
4.8. Cell Proliferation Assay
4.9. TUNEL Assay
4.10. Collagen Synthesis by HDFs
4.11. qPCR Analysis
4.12. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lin, Z.; Shibuya, Y.; Imai, Y.; Oshima, J.; Sasaki, M.; Sasaki, K.; Aihara, Y.; Khanh, V.C.; Sekido, M. Therapeutic Potential of Adipose-Derived Stem Cell-Conditioned Medium and Extracellular Vesicles in an In Vitro Radiation-Induced Skin Injury Model. Int. J. Mol. Sci. 2023, 24, 17214. https://doi.org/10.3390/ijms242417214
Lin Z, Shibuya Y, Imai Y, Oshima J, Sasaki M, Sasaki K, Aihara Y, Khanh VC, Sekido M. Therapeutic Potential of Adipose-Derived Stem Cell-Conditioned Medium and Extracellular Vesicles in an In Vitro Radiation-Induced Skin Injury Model. International Journal of Molecular Sciences. 2023; 24(24):17214. https://doi.org/10.3390/ijms242417214
Chicago/Turabian StyleLin, Zhixiang, Yoichiro Shibuya, Yukiko Imai, Junya Oshima, Masahiro Sasaki, Kaoru Sasaki, Yukiko Aihara, Vuong Cat Khanh, and Mitsuru Sekido. 2023. "Therapeutic Potential of Adipose-Derived Stem Cell-Conditioned Medium and Extracellular Vesicles in an In Vitro Radiation-Induced Skin Injury Model" International Journal of Molecular Sciences 24, no. 24: 17214. https://doi.org/10.3390/ijms242417214