Genome-Wide Identification of the ERF Transcription Factor Family for Structure Analysis, Expression Pattern, and Response to Drought Stress in Populus alba × Populus glandulosa
Abstract
:1. Introduction
2. Results
2.1. Identification of the PagERF Transcription Factor Family in P. alba × P. glandulosa
2.2. Chromosomal Distribution of PagERF Genes
2.3. Phylogenetic Analysis of PagERF Proteins
2.4. Localization and Duplication of PagERF Genes
2.5. Analysis of PagERF Gene Structure and PagERF Motif Composition
2.6. Analysis of Cis-Acting Elements in PagERF Promoters
2.7. Expression Pattern Analysis of PagERF Genes
2.8. RT-qPCR Validation of PagERF Gene Expression Patterns
2.9. Analysis of PagERF Genes in Response to Drought in Seedlings
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Treatments
4.2. Identification of PagERF Transcription Factors
4.3. Chromosomal Localization and Evolutionary Analysis of PagERF Transcription Factors
4.4. Motif Analysis of PagERF Transcription Factors and Phylogenetic Tree Reconstruction
4.5. Analysis of Cis-Acting Elements in PagERF Promoters
4.6. RNA Extraction and RT-qPCR
4.7. RNA Sequencing
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zeng, D.; Dai, L.-J.; Li, X.; Li, W.; Qu, G.-Z.; Li, S. Genome-Wide Identification of the ERF Transcription Factor Family for Structure Analysis, Expression Pattern, and Response to Drought Stress in Populus alba × Populus glandulosa. Int. J. Mol. Sci. 2023, 24, 3697. https://doi.org/10.3390/ijms24043697
Zeng D, Dai L-J, Li X, Li W, Qu G-Z, Li S. Genome-Wide Identification of the ERF Transcription Factor Family for Structure Analysis, Expression Pattern, and Response to Drought Stress in Populus alba × Populus glandulosa. International Journal of Molecular Sciences. 2023; 24(4):3697. https://doi.org/10.3390/ijms24043697
Chicago/Turabian StyleZeng, Dong, Li-Juan Dai, Xiang Li, Wei Li, Guan-Zheng Qu, and Shuang Li. 2023. "Genome-Wide Identification of the ERF Transcription Factor Family for Structure Analysis, Expression Pattern, and Response to Drought Stress in Populus alba × Populus glandulosa" International Journal of Molecular Sciences 24, no. 4: 3697. https://doi.org/10.3390/ijms24043697