Antitumor Effect of Cycloastragenol in Colon Cancer Cells via p53 Activation
Abstract
:1. Introduction
2. Results
2.1. Cycloastragenol Inhibits Colon Cancer Cell Viability
2.2. Cycloastragenol Induces p53 and Decreases Caspase 3 Expression
2.3. Cycloastragenol Induces Time-Dependent Apoptosis in HCT116p53+/+ Cells
2.4. p53 Stability Was Confirmed by Treatment with Cycloheximide in the HCT116p53+/+ Cells
2.5. The Role of p53 in CY-Mediated Apoptosis
2.6. Combinational Effect with CY and 5-FU or Doxorubicin
2.7. Ribosomal Protein RPL5 Mediates p53 Activation Due to CY in HCT116p53+/+ Cells
2.8. CY Alleviated Induced 5-FU Resistance of CRC Cells
2.9. CY Induces DNA Damage and Activation of ERK Kinase, p38 MAPK, and JNK
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Cytotoxicity Assay
4.3. Cell Cultures
4.4. p53 Stability Assay Using Cycloheximide
4.5. Isolation of the Total RNA and Real-Time PCR
4.6. Western Blotting
4.7. Terminal Deoxynucleotidyl Transferase-Mediated dUTP Nick end Labeling (TUNEL) Assay
4.8. siRNA Transfection
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Park, D.; Jung, J.H.; Ko, H.M.; Jee, W.; Kim, H.; Jang, H.-J. Antitumor Effect of Cycloastragenol in Colon Cancer Cells via p53 Activation. Int. J. Mol. Sci. 2022, 23, 15213. https://doi.org/10.3390/ijms232315213
Park D, Jung JH, Ko HM, Jee W, Kim H, Jang H-J. Antitumor Effect of Cycloastragenol in Colon Cancer Cells via p53 Activation. International Journal of Molecular Sciences. 2022; 23(23):15213. https://doi.org/10.3390/ijms232315213
Chicago/Turabian StylePark, Doil, Ji Hoon Jung, Hyun Min Ko, Wona Jee, Hyungsuk Kim, and Hyeung-Jin Jang. 2022. "Antitumor Effect of Cycloastragenol in Colon Cancer Cells via p53 Activation" International Journal of Molecular Sciences 23, no. 23: 15213. https://doi.org/10.3390/ijms232315213