Therapeutic Effect of Mitochondrial Division Inhibitor-1 (Mdivi-1) on Hyperglycemia-Exacerbated Early and Delayed Brain Injuries after Experimental Subarachnoid Hemorrhage
Abstract
:1. Introduction
2. Results
2.1. ELISA Assay for Inflammatory Factors In Vitro
2.2. Neurological Outcomes
2.3. Morphological, Cross-Sectional-Area, and Thickness Changes in Basal Artery (BA)
2.4. Microglia and Astrocyte Proliferation
2.5. Real-Time PCR of Pro-Inflammatory Factors
2.6. Apoptosis of Neuron Cells
2.7. Western Blot Analysis
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Treatment
4.2. Animal Preparation
4.3. Hyperglycemia Induction
4.4. SAH Induction
4.5. Experimental Design and Drug Administration
4.6. Neurological Assessment
4.7. Tissue Processing
4.8. Morphometric Assessment of BA
4.9. Immunofluorescence
4.10. Enzyme-Linked Immunosorbent Assay (ELISA)
4.11. TUNEL Staining
4.12. Western Blot Analysis
4.13. Real-Time Quantitative PCR (qRT-PCR)
4.14. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Group | Ambulation | Placing/Stepping Reflex | MDI |
---|---|---|---|
Normal | 0 | 0 | 0 |
SAH | 1.91 ± 0.37 | 1.34 ± 0.11 | 2.86 ± 0.24 |
DM + SAH | 2.39 ± 0.27 | 1.77 ± 0.19 | 4.20 ± 0.31 |
DM + SAH + Mdivi-1 (0.24 mg/kg) | 2.11 ± 0.19 | 1.59 ± 0.29 | 3.83 ± 0.25 |
DM + SAH + Mdivi-1 (1.2 mg/kg) | 1.62 ± 0.13 * | 0.57 ± 0.21 * | 1.89 ± 0.31 * |
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Chung, C.-L.; Huang, Y.-H.; Lin, C.-J.; Chong, Y.-B.; Wu, S.-C.; Chai, C.-Y.; Tsai, H.-P.; Kwan, A.-L. Therapeutic Effect of Mitochondrial Division Inhibitor-1 (Mdivi-1) on Hyperglycemia-Exacerbated Early and Delayed Brain Injuries after Experimental Subarachnoid Hemorrhage. Int. J. Mol. Sci. 2022, 23, 6924. https://doi.org/10.3390/ijms23136924
Chung C-L, Huang Y-H, Lin C-J, Chong Y-B, Wu S-C, Chai C-Y, Tsai H-P, Kwan A-L. Therapeutic Effect of Mitochondrial Division Inhibitor-1 (Mdivi-1) on Hyperglycemia-Exacerbated Early and Delayed Brain Injuries after Experimental Subarachnoid Hemorrhage. International Journal of Molecular Sciences. 2022; 23(13):6924. https://doi.org/10.3390/ijms23136924
Chicago/Turabian StyleChung, Chia-Li, Yu-Hua Huang, Chien-Ju Lin, Yoon-Bin Chong, Shu-Chuan Wu, Chee-Yin Chai, Hung-Pei Tsai, and Aij-Lie Kwan. 2022. "Therapeutic Effect of Mitochondrial Division Inhibitor-1 (Mdivi-1) on Hyperglycemia-Exacerbated Early and Delayed Brain Injuries after Experimental Subarachnoid Hemorrhage" International Journal of Molecular Sciences 23, no. 13: 6924. https://doi.org/10.3390/ijms23136924