Divergent Mutational Landscapes of Consensus and Minority Genotypes of West Nile Virus Demonstrate Host and Gene-Specific Evolutionary Pressures
Abstract
:1. Introduction
2. Materials and Methods
3. Results
3.1. Host and Gene-Specific Genetic Diversity and Selective Pressures
3.2. Unique Intra and Interhost Genetic Signatures
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Interhost | |||||
AA 1 | NT 2 | pN 3 | Mean Sn NT | Mean Sn AA | |
All | 4702 | 25,503 | 0.1844 | 0.0023 | 0.0005 |
Avian | 1896 | 10,739 | 0.1766 | 0.0019 | 0.0004 |
Mosquito | 2796 | 14,671 | 0.1906 | 0.0028 | 0.0007 |
Intrahost | |||||
AA 1 | NT 2 | pN 3 | Mean Sn NT | Mean Sn AA | |
All | 151,265 | 380,537 | 0.3975 | 0.0346 | 0.0192 |
Avian | 113,728 | 283,958 | 0.4005 | 0.0470 * | 0.0277 * |
Mosquito | 36,449 | 91,853 | 0.3968 | 0.0233 * | 0.0114 * |
Intrahost Substitution Ratio | ||||||||||||
# A - U | # A - C | # A - G | # U - A | # U - G | # U - C | # G - A | # G - C | # G - U | # C - U | # C - G | # C - A | |
All | 0.0966 | 0.0866 | 0.0954 | 0.0674 | 0.0597 | 0.0777 | 0.0948 | 0.0910 | 0.0934 | 0.0932 | 0.0717 | 0.0726 |
Avian | 0.0969 | 0.0868 | 0.0943 | 0.0671 | 0.0597 | 0.0770 | 0.0943 | 0.0935 | 0.0943 | 0.0918 | 0.0715 | 0.0727 |
Mosquito | 0.0957 | 0.0858 | 0.0995 | 0.0679 | 0.0594 | 0.0799 | 0.0967 | 0.0834 | 0.0903 | 0.0971 | 0.0724 | 0.0718 |
Interhost Substitution Ratio | ||||||||||||
# A - U | # A - C | # A - G | # U - A | # U - G | # U - C | # G - A | # G - C | # G - U | # C - U | # C - G | # C - A | |
All | 0.0462 | 0.0151 | 0.1087 | 0.0325 | 0.0038 | 0.2592 | 0.1169 | 0.0031 | 0.0151 | 0.3879 | 0.0013 | 0.0100 |
Avian | 0.0549 | 0.0092 | 0.1187 | 0.0359 ns | 0.0049 | 0.2701 | 0.1104 | 0.0023 | 0.0131 | 0.3716 | 0.0009 | 0.0076 |
Mosquito | 0.0398 * | 0.0195 | 0.1018 | 0.0300 | 0.0030 | 0.2508 | 0.1219 | 0.0037 | 0.0167 | 0.3998 | 0.0016 | 0.0116 |
Interhost | Intrahost | ||
---|---|---|---|
Mutation | Significance | Mutation | Significance |
# A - U | a **** | # A - U | ns |
# A - C | m **** | # A - C | ns |
# A - G | a **** | # A - G | m **** |
# U - A | a ** | # U - A | ns |
# U - G | a * | # U - G | ns |
# U - C | a **** | # U - C | m ** |
# G - A | a **** | # G - A | m * |
# G - C | ns | # G - C | a **** |
# G - U | m * | # G - U | a *** |
# C - U | m **** | # C - U | m **** |
# C - G | ns | # C - G | ns |
# C - A | m ** | # C - A | ns |
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Caldwell, H.S.; Lasek-Nesselquist, E.; Follano, P.; Kramer, L.D.; Ciota, A.T. Divergent Mutational Landscapes of Consensus and Minority Genotypes of West Nile Virus Demonstrate Host and Gene-Specific Evolutionary Pressures. Genes 2020, 11, 1299. https://doi.org/10.3390/genes11111299
Caldwell HS, Lasek-Nesselquist E, Follano P, Kramer LD, Ciota AT. Divergent Mutational Landscapes of Consensus and Minority Genotypes of West Nile Virus Demonstrate Host and Gene-Specific Evolutionary Pressures. Genes. 2020; 11(11):1299. https://doi.org/10.3390/genes11111299
Chicago/Turabian StyleCaldwell, Haley S., Erica Lasek-Nesselquist, Paisley Follano, Laura D. Kramer, and Alexander T. Ciota. 2020. "Divergent Mutational Landscapes of Consensus and Minority Genotypes of West Nile Virus Demonstrate Host and Gene-Specific Evolutionary Pressures" Genes 11, no. 11: 1299. https://doi.org/10.3390/genes11111299