Insights into Intramuscular Connective Tissue Associated with Wooden Breast Myopathy in Fast-Growing Broiler Chickens
Abstract
:1. Introduction
2. Materials and Methods
2.1. Broiler Chicken Breast Fillets
2.2. Extraction of Intramuscular Connective Tissue and Protein Solution Preparation
2.3. Histological Evaluation
2.4. Transmission Electron Microscope (TEM) Observation
2.5. Mechanical Strength Measurement
2.6. Differential Scanning Calorimetry (DSC) Analysis
2.7. FT-IR Spectroscopy
2.8. Particle Size and Molecular Weight Distribution of Connective Tissue Protein
2.9. Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)
2.10. Measurement of Surface Hydrophobicity
2.11. Measurement of Intrinsic Fluorescence
2.12. Statistical Analysis
3. Results and Discussion
3.1. Collagen Organization
3.2. Collagen Fibrillar Structure
3.3. Mechanical Strength
3.4. Thermogram of the Connective Tissue
3.5. Secondary Structure
3.6. Particle Size
3.7. Surface Hydrophobicity
3.8. Intrinsic Fluorescence
3.9. OPLS-DA Model
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Severity | TO (°C) | TM (°C) | TE (°C) | ΔH (J/g) |
---|---|---|---|---|
NOR | 61.53 c | 66.46 c | 77.20 b | 4.95 a |
MIL | 63.40 b | 67.19 bc | 77.62 b | 2.41 b |
MOD | 64.66 b | 68.35 b | 79.25 a | 2.34 b |
SEV | 67.50 a | 70.18 a | 80.88 a | 2.25 b |
s.e.m. | 0.24 | 0.60 | 0.37 | 0.42 |
p value | <0.0001 | <0.0001 | <0.0001 | <0.0001 |
Myopathy Level | α-Helix (%) | β-Sheet (%) | β-Turn (%) | Random Coil (%) |
---|---|---|---|---|
NOR | 14.61 a | 45.71 a | 20.78 c | 19.64 b |
MIL | 12.98 b | 41.17 b | 24.64 ab | 21.24 b |
MOD | 11.64 c | 36.40 c | 23.24 b | 28.67 a |
SEV | 11.54 c | 32.80 d | 25.08 a | 29.61 a |
s.e.m. | 0.50 | 1.57 | 1.19 | 1.41 |
p value | <0.0001 | <0.0001 | 0.0003 | <0.0001 |
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Zhang, Y.; Huang, M.; Shao, X.; Zhang, F.; Li, Z.; Bai, Y.; Xu, X.; Wang, P.; Zhao, T. Insights into Intramuscular Connective Tissue Associated with Wooden Breast Myopathy in Fast-Growing Broiler Chickens. Foods 2023, 12, 2375. https://doi.org/10.3390/foods12122375
Zhang Y, Huang M, Shao X, Zhang F, Li Z, Bai Y, Xu X, Wang P, Zhao T. Insights into Intramuscular Connective Tissue Associated with Wooden Breast Myopathy in Fast-Growing Broiler Chickens. Foods. 2023; 12(12):2375. https://doi.org/10.3390/foods12122375
Chicago/Turabian StyleZhang, Yulong, Mingyuan Huang, Xuefei Shao, Feiyu Zhang, Zhen Li, Yun Bai, Xinglian Xu, Peng Wang, and Tinghui Zhao. 2023. "Insights into Intramuscular Connective Tissue Associated with Wooden Breast Myopathy in Fast-Growing Broiler Chickens" Foods 12, no. 12: 2375. https://doi.org/10.3390/foods12122375