Characterization of Lactic Acid Bacteria Strains Isolated from Algerian Honeybee and Honey and Exploration of Their Potential Probiotic and Functional Features for Human Use
Abstract
:1. Introduction
2. Materials and Methods
2.1. Honeybees and Honey Sampling
2.2. Isolation of Acid Tolerant LAB and Characterization of Fermentation Pattern
2.3. Molecular Identification and Phylogenetic Analysis
2.4. Assessment of Proteolytic, Lipolytic Activities and EPS Production
2.5. Resistance to Simulated Gastro-Intestinal Conditions
2.6. Autoaggregation and Cell Surface Hydrophobicity
2.7. Antimicrobial Activity
2.8. In Vitro Cholesterol Lowering Ability
2.9. Antibiotic Susceptibility
2.10. Hemolytic Activity
2.11. Biogenic Amines Production
2.12. Statistical Analysis
3. Results and Discussion
3.1. Physico-Chemical Characterization of Honey Samples
3.2. Molecular Identification and Phylogenetic Analysis
3.3. Biochemical Characterization
3.4. Assessment of Proteolytic, Lipolytic Activities and EPS Production
3.5. Resistance to Simulated Gastro-Intestinal Conditions
3.6. Autoaggregation and Cell Surface Hydrophobicity
3.7. Antimicrobial Activity
3.8. In Vitro Cholesterol Lowering Ability
3.9. Antibiotic Susceptibility
3.10. Hemolysis
3.11. Biogenic Amines Production
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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U21 | AB1 | 49d | AB24 | AB34 | ML10 | AB5 | AB18 | AB46 | 4M | AM | FM | QM | MM | ML7 | ML17 | ML20 | AB36 | AB70 | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
L-arabinose | - | - | - | - | - | - | + | - | - | - | w | - | - | - | - | - | - | - | - |
D-ribose | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + |
D-galactose | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + |
D-glucose | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + |
D-fructose | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + |
D-monose | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + |
L-rhamnose | - | - | - | - | - | - | w | - | - | - | - | - | - | - | - | - | - | - | - |
D-mannitol | w | w | - | w | w | - | + | - | - | + | + | + | + | + | - | - | - | + | + |
N-acetylglucosamine | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + |
Amygdaline | + | + | + | + | + | + | + | w | w | + | + | + | + | + | + | + | + | + | + |
Arbutine | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + |
Esculine citrate de fer | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + |
Salicine | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + |
D-celobiose | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + |
D-maltose | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + |
D-lactose(bovine) | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + |
D-melibiose | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + |
D-saccharose | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + |
D-trehalose | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + |
D-raffinose | w | w | w | w | w | - | w | w | w | + | + | + | + | + | + | + | + | + | + |
Amidon | - | - | - | - | - | - | - | w | w | - | - | - | - | - | - | - | - | - | - |
Gentiobiose | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + |
D-tagatose | - | - | - | - | - | - | - | + | + | - | w | - | - | - | - | - | w | - | - |
Potassium gluconate | w | w | - | w | - | - | - | - | - | w | w | - | - | - | - | - | w | - | - |
U21 | AB1 | 49d | ML10 | AB5 | AB46 | 4M | AM | FM | QM | MM | ML7 | AB36 | ML20 | ||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
K. pneumonia ATCC 70060 | BC | 20.0 ± 0.1 | 23.00 ± 0.06 | 19.0 ± 0.06 | 18.50 ± 0.03 | 18.50 ± 0.03 | 15.50 ± 0.03 | 23.0 ± 0.2 | 10.0 ± 0.0 | 12.0 ± 0.2 | 11.50 ± 0.03 | 14.6 ± 0.3 | 23.0 ± 0.1 | 23.0 ± 0.2 | 20.0 ± 0.1 |
CFS | - | - | - | - | - | - | - | - | - | - | - | - | - | - | |
B. cereus ATCC 10876 | BC | 16.0 ± 0.1 | 19.5 ± 0.03 | 15.0 ± 0.2 | 22.0 ± 0.1 | 19.30 ± 0.04 | 13.5 ± 0.1 | 20.30 ± 0.04 | 16.0 ± 0.2 | 17.50 ± 0.03 | 14.0 ± 0.1 | 12.30 ± 0.08 | 13.0 ± 0.1 | 17.0 ± 0.3 | 23.0 ± 0.4 |
CFS | - | - | - | - | - | - | - | - | - | - | - | - | - | 22.5 ± 0.5 | |
S. aureus ATCC 43300 | BC | 23.0 ± 0.4 | 20.0 ± 0.1 | 15.0 ± 0.2 | 22.3 ± 0.1 | 17.10 ± 0.05 | 16.0 ± 0.2 | 21.0 ± 0.2 | 14.0 ± 0.4 | 16.5 ± 0.1 | 19.0 ± 0.3 | 19.0 ± 0.2 | 19.0 ± 0.2 | 19.0 ± 0.3 | 18.0 ± 0.1 |
CFS | 15.0 ± 0.6 | - | - | - | - | - | - | - | - | - | - | - | - | - | |
E. coli ATCC 25522 | BC | 15.0 ± 0.3 | 12.5 ± 0.1 | - | 27.6 ± 0.4 | 14.0 ± 0.2 | - | 21.0 ± 0.1 | - | 16.0 ± 0.2 | 14.6 ± 0.1 | 14.0 ± 0.06 | 13.0 ± 0.2 | 19.0 ± 0.6 | 18.0 ± 0.1 |
CFS | - | - | - | - | - | - | - | - | - | - | - | - | - | 10.0 ± 0.2 | |
P. aeruginosa ATCC 27853 | BC | 19.0 ± 0.3 | 14.0 ± 0.2 | - | 11.0 ± 0.2 | 13.3 ± 0.2 | - | 13.0 ± 0.2 | 10.6 ± 0.1 | 14.0 ± 0.2 | 10.00 ± 0.06 | 12.6 ± 0.2 | 08.00 ± 0.06 | 15.0 ± 0.2 | 14.0 ± 0.2 |
CFS | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
K. pneumonia ATCC 700603 | B. cereus ATCC 10876 | S. aureus ATCC 43300 | E. coli ATCC 25522 | P. aeruginosa ATCC 27853 | |
---|---|---|---|---|---|
Ampicillin | - | - | - | - | - |
Cefoxitin | 12.0 ± 0.8 | - | 10.0 ± 0.1 | - | - |
Chloranohenicol | 12.0 ± 0.3 | - | 18.0 ± 1.1 | - | - |
Clindamicyn | - | - | - | - | - |
Oxacillin | - | - | - | - | - |
Penicillin | - | - | - | - | - |
Tetracycline | - | 28.0 ± 0.6 | 23.00 ± 0.06 | - | - |
Trimethoprim | - | - | 16.0 ± 0.5 | - | - |
Vancomycin | - | - | 14.0 ± 1.0 | - | - |
Erytromicyn | - | - | - | - | - |
Naladixic acid | - | - | 13.0 ± 0.2 | - | 14.00 ± 0.08 |
Streptomycin | 19.0 ± 0.9 | 28.00 ± 0.03 | 20.00 ± 0.04 | 20.0 ± 0.3 | 13.0 ± 0.4 |
Gentamycin | 14.00 ± 0.07 | 14.0 ± 1.0 | 23.0 ± 0.3 | 13.0 ± 0.4 | 16.0 ± 0.3 |
U21 | AB1 | 49d | ML10 | AB5 | AB46 | 4M | AM | FM | QM | MM | ML7 | AB36 | ML20 | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Ampicillin | S | S | S | S | S | S | S | S | S | S | S | S | S | S |
Cefoxitin | S | S | S | I | S | S | I | S | S | S | I | I | I | S |
Chloranohenicol | S | S | S | S | S | S | S | S | S | S | S | S | S | S |
Clindamicyn | S | S | S | S | S | S | S | S | S | S | S | S | S | S |
Oxacillin | R | R | R | R | R | R | R | R | R | R | R | R | R | R |
Penicillin | S | R | R | R | R | S | R | S | R | R | I | I | R | I |
Tetracycline | R | I | I | R | R | I | I | R | R | R | R | R | I | I |
Trimethoprim | S | R | R | R | R | R | I | R | I | I | I | I | I | I |
Vancomycin | R | R | R | R | R | R | R | R | R | R | R | R | R | R |
Erythromicyn | S | S | S | S | S | S | I | S | S | S | S | S | I | S |
Naladixic acid | R | R | R | R | R | R | R | R | R | R | R | R | R | R |
Streptomycin | R | I | R | R | I | I | S | S | R | I | R | R | I | S |
Gentamycin | R | R | R | R | R | I | R | R | R | R | R | R | R | I |
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Meradji, M.; Bachtarzi, N.; Mora, D.; Kharroub, K. Characterization of Lactic Acid Bacteria Strains Isolated from Algerian Honeybee and Honey and Exploration of Their Potential Probiotic and Functional Features for Human Use. Foods 2023, 12, 2312. https://doi.org/10.3390/foods12122312
Meradji M, Bachtarzi N, Mora D, Kharroub K. Characterization of Lactic Acid Bacteria Strains Isolated from Algerian Honeybee and Honey and Exploration of Their Potential Probiotic and Functional Features for Human Use. Foods. 2023; 12(12):2312. https://doi.org/10.3390/foods12122312
Chicago/Turabian StyleMeradji, Meriem, Nadia Bachtarzi, Diego Mora, and Karima Kharroub. 2023. "Characterization of Lactic Acid Bacteria Strains Isolated from Algerian Honeybee and Honey and Exploration of Their Potential Probiotic and Functional Features for Human Use" Foods 12, no. 12: 2312. https://doi.org/10.3390/foods12122312