Histological and Immunohistochemical Analysis of the Effects of Topical Melatonin Treatment Associated with Collagen Sponge and rhBMP-2 Protein on Bone Remodeling
Abstract
:1. Introduction
2. Materials and Methods
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Groups | Treatments |
---|---|
Group 1 | blood clot |
Group 2 | blood clot + Scaffold |
Group 3 | blood clot + Scaffold + Melatonin 1 µg |
Group 4 | blood clot + Scaffold + Melatonin 10 µg |
Group 5 | blood clot + Scaffold + Melatonin 100 µg |
Group 6 | blood clot + Scaffold + Melatonin 1 µg + rhBMP-2 |
Group 7 | blood clot + Scaffold + Melatonin 10 µg + rhBMP-2 |
Group 8 | blood clot + Scaffold + Melatonin 100 µg + rhBMP-2 |
Group 9 | blood clot + Scaffold + rhBMP-2 |
Group 1 | Group 2 | Group 3 | Group 4 | Group 5 | Group 6 | Group 7 | Group 8 | Group 9 | |
---|---|---|---|---|---|---|---|---|---|
OPG | ++++ | + | +++ | +++ | +++ | ++++ | ++ | ++++ | +++ |
CD31 | + | + | + | + | + | + | + | + | + |
IL1β | ++ | ++ | ++ | +++ | ++ | ++ | ++ | ++ | ++ |
IL6 | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
IL10 | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
Osteocalcin | +++ | + | ++ | ++ | ++ | +++ | +++ | +++ | +++ |
Osteoprogeterin | ++++ | ++ | ++ | ++ | ++ | ++++ | +++ | ++++ | ++++ |
RUNX2 | ++ | + | ++ | ++ | ++ | +++ | ++ | +++ | +++ |
TRAP | ++ | + | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
VEGF | ++ | ++ | ++ | ++ | ++ | ++ | +++ | ++ | ++ |
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Montarele, L.F.; Pitol, D.L.; Pereira, B.F.; Feldman, S.; Fazan, V.P.S.; Issa, J.P.M. Histological and Immunohistochemical Analysis of the Effects of Topical Melatonin Treatment Associated with Collagen Sponge and rhBMP-2 Protein on Bone Remodeling. Biomolecules 2022, 12, 1738. https://doi.org/10.3390/biom12121738
Montarele LF, Pitol DL, Pereira BF, Feldman S, Fazan VPS, Issa JPM. Histological and Immunohistochemical Analysis of the Effects of Topical Melatonin Treatment Associated with Collagen Sponge and rhBMP-2 Protein on Bone Remodeling. Biomolecules. 2022; 12(12):1738. https://doi.org/10.3390/biom12121738
Chicago/Turabian StyleMontarele, Leticia Ferreira, Dimitrius Leonardo Pitol, Bruno Fiorelini Pereira, Sara Feldman, Valéria Paula Sassoli Fazan, and João Paulo Mardegan Issa. 2022. "Histological and Immunohistochemical Analysis of the Effects of Topical Melatonin Treatment Associated with Collagen Sponge and rhBMP-2 Protein on Bone Remodeling" Biomolecules 12, no. 12: 1738. https://doi.org/10.3390/biom12121738