Possibility of Limiting Mineral Fertilization in Potato Cultivation by Using Bio-fertilizer and Its Influence on Protein Content in Potato Tubers
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Location and Treatment
- K–hydrothermal coefficient value,
- P–total monthly precipitation,
- t–total mean daily temperature in a given month.
2.2. Plant Sampling and Chemical Analysis
2.3. Statistical Analysis
3. Results
3.1. Crude Protein Content in Potato Tubers
3.2. Essential and Non-Essential Amino Acids Content in Potato Tubers
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Type of Organic Sources (1st Factor) | |
---|---|
Control | without organic sources |
Catch crop (fodder pea) | 27 t ha−1, in autumn, after post-harvest treatments and before pre-winter plough |
Straw | 4 t ha−1, in autumn, after post-harvest treatments and before pre-winter plough |
Farmyard manure (FYM) | 25 t ha−1, in autumn, before pre-winter plough |
Rate of Mineral Fertilization (2nd Factor) (Ammonium Nitrate-34%, Triple Superphosphate-46%, Potassium Sulphate-50%) | |
100% NPK 50% NPK | 100 kg N ha−1, 100 kg P2O5 ha−1, 150 kg K2O ha−1 50 kg N ha−1, 50 kg P2O5 ha−1, 150 kg K2O ha−1 |
applied in spring, prior to potato planting, at the rates adjusted to the soil richness and nutritive requirements of the plant | |
Bio-Fertilizer Application (3rd Factor) | |
Control | Without treatment |
Bio-fertilizer | UGmax fertilizer applied at three rates: 0.6 l ha−1 in autumn, on organic sources, prior to pre-winter plough; 0.3 l ha−1 in spring, prior to tuber planting, during soil tillage; 0.3 l ha−1 as foliar fertilization, at the plant height of 15–20 cm |
Parameters | Unit | Value | Categories |
---|---|---|---|
pH H2O pH KCl | - - | 5.1–6.7 5.7–6.1 | Slightly acid |
Organic carbon | g kg−1 | 7.55–7.80 | - |
Total nitrogen | g kg−1 | 0.69–0.75 | Low richness |
Phosphorus | mg kg−1 | 190.0–210.0 | High richness |
Potassium | mg kg−1 | 95.0–150.0 | Medium richness |
Magnesium | mg kg−1 | <20.0 | Very low richness |
Component | Organic Source [kg·t−1] | Organic Source [t·ha−1] | ||||
---|---|---|---|---|---|---|
Catch Crop | Straw | Farmyard Manure | Catch Crop | Straw | Farmyard Manure | |
N | 35.10 | 5.62 | 5.12 | 488.7 | 151.74 | 138.24 |
P2O5 | 4.34 | 1.06 | 4.48 | 90.18 | 28.62 | 120.96 |
K2O | 25.15 | 10.21 | 6.85 | 247.05 | 275.67 | 184.95 |
CaO | 2.74 | 0.84 | 4.42 | 46.98 | 22.68 | 119.34 |
Mg | 7.25 | 2.25 | 1.82 | 141.75 | 33.75 | 49.14 |
Years | Rainfall | Total | |||||
---|---|---|---|---|---|---|---|
Month | |||||||
April | May | June | July | August | September | ||
2009 | 0.4 | 12.4 | 53.4 | 118.0 | 17.6 | 34.4 | 236.1 |
2010 | 33.8 | 92.6 | 18.1 | 107.4 | 150.7 | 74.7 | 477.3 |
2011 | 13.5 | 35.4 | 100.8 | 132.5 | 67.7 | 37.0 | 386.9 |
Average | 15.9 | 46.8 | 57.4 | 119.3 | 78.7 | 48.7 | 366.8 |
Temperature | |||||||
2009 | 9.8 | 12.3 | 14.5 | 18.6 | 18.2 | 13.7 | 87.1 |
2010 | 7.8 | 11.5 | 16.7 | 21.6 | 18.4 | 12.2 | 88.2 |
2011 | 10.5 | 13.5 | 17.7 | 17.5 | 17.7 | 14.3 | 91.2 |
Average | 9.4 | 12.4 | 16.3 | 19.2 | 18.1 | 13.4 | 88.8 |
Hydrothermal Coefficient K | Average | ||||||
2009 | 0.01 | 0.32 | 1.23 | 2.05 | 0.61 | 0.84 | 0.84 |
2010 | 1.44 | 2.60 | 0.36 | 1.60 | 2.64 | 2.04 | 1.78 |
2011 | 0.43 | 0.85 | 1.90 | 2.44 | 1.23 | 0.86 | 1.29 |
Average | 0.63 | 1.25 | 1.16 | 2.03 | 1.50 | 1.25 | 1.30 |
Essential Amino Acids | Organic Sources | Mineral Fertilizers | Bio-Fertilizer | Organic Sources × Mineral Fertilizers | Organic Sources × Bio-Fertilizer | Mineral Fertilizers × Bio-Fertilizer | Organic Sources × Mineral Fertilizers × Bio-Fertilizer |
---|---|---|---|---|---|---|---|
Tyrosine | * | * | * | * | * | * | ** |
Threonine | * | * | * | ** | ** | * | * |
Methionine | ** | n.s. | ** | * | * | ** | * |
Valine | ** | n.s. | ** | ** | ** | ** | ** |
Isoleucine | ** | n.s. | ** | ** | ** | ** | ** |
Leucine | * | n.s. | ** | ** | ** | ** | ** |
Lysine | ** | ** | ** | ** | ** | ** | ** |
Phenylalanine | ** | n.s. | * | ** | ** | ** | ** |
Sum | ** | n.s. | ** | ** | ** | ** | ** |
Non-Essential Amino Acids | Organic Sources | Mineral Fertilizers | Bio-Fertilizer | Organic Sources × Mineral Fertilizers | Organic Sources × Bio-Fertilizer | Mineral Fertilizers × Bio-Fertilizer | Organic Sources × Mineral Fertilizers × Bio-Fertilizer |
---|---|---|---|---|---|---|---|
Aspartic acid | ** | ** | ** | ** | n.s. | ** | ** |
Asparagine | ** | ** | ** | ** | ** | ** | ** |
Alanine | ** | ** | ** | ** | ** | ** | ** |
Glutamic acid | ** | n.s. | ** | * | * | n.s. | ** |
Sum | ** | * | ** | ** | ** | ** | ** |
Organic Fertilization | Mineral NPK Fertilization | Bio-Fertilizer | Tyrosine | Threonine | Methionine | Valine | Isoleucine | Leucine | Lysine | Phenyl- Alanine | Sum |
---|---|---|---|---|---|---|---|---|---|---|---|
Control (without organic source) | 100% | Control | 0.64 ± 0.01 | 1.40 ± 0.08 | 2.19 ± 0.11 | 2.40 ± 0.14 | 1.84 ± 0.05 | 0.52 ± 0.04 | 0.17 ± 0.01 | 1.66 ± 0.04 | 10.82 |
Bio-fertilizer | 0.71 ± 0.02 | 1.32 ± 0.02 | 2.34 ± 0.05 | 2.54 ± 0.08 | 1.95 ± 0.11 | 1.72 ± 0.02 | 0.64 ± 0.0 | 1.39 ± 0.01 | 12.61 | ||
50% | Control | 0.83 ± 0.01 | 1.26 ± 0.01 | 2.09 ± 0.08 | 2.30 ± 0.01 | 1.57 ± 0.08 | 2.27 ± 0.02 | 0.09 ± 0.01 | 1.65 ± 0.01 | 12.06 | |
Bio-fertilizer | 0.70 ± 0.01 | 1.28 ± 0.04 | 2.29 ± 0.03 | 2.31 ± 0.01 | 1.67 ± 0.05 | 2.31 ± 0.03 | 0.11 ± 0.03 | 1.26 ± 0.01 | 11.93 | ||
Catch crop (pea) | 100% | Control | 1.36 ± 0.02 | 2.49 ± 0.03 | 2.67 ± 0.07 | 3.89 ± 0.05 | 2.94 ± 0.04 | 4.67 ± 0.11 | 0.22 ± 0.02 | 2.39 ± 0.01 | 20.63 |
Bio-fertilizer | 1.06 ± 0.01 | 3.87 ± 0.11 | 3.05 ± 0.04 | 4.06 ± 0.04 | 3.41 ± 0.06 | 4.97 ± 0.08 | 0.89 ± 0.01 | 2.25 ± 0.02 | 23.56 | ||
50% | Control | 1.12 ± 0.05 | 2.21 ± 0.05 | 2.58 ± 0.05 | 3.72 ± 0.02 | 3.50 ± 0.07 | 3.92 ± 0.07 | 0.23 ± 0.01 | 2.25 ± 0.01 | 19.53 | |
Bio-fertilizer | 1.35 ± 0.03 | 3.43 ± 0.08 | 2.96 ± 0.08 | 3.78 ± 0.01 | 3.57 ± 0.04 | 4.59 ± 0.02 | 0.44 ± 0.01 | 2.12 ± 0.01 | 22.24 | ||
Straw | 100% | Control | 1.18 ± 0.04 | 2.69 ± 0.02 | 2.34 ± 0.05 | 2.89 ± 0.03 | 3.03 ± 0.08 | 4.82 ± 0.03 | 0.31 ± 0.00 | 2.45 ± 0.01 | 19.71 |
Bio-fertilizer | 1.62 ± 0.01 | 4.52 ± 0.05 | 2.38 ± 0.03 | 2.94 ± 0.11 | 3.98 ± 0.11 | 3.45 ± 0.05 | 0.99 ± 0.01 | 2.51 ± 0.02 | 24.79 | ||
50% | Control | 0.91 ± 0.01 | 1.57 ± 0.02 | 2.28 ± 0.01 | 2.55 ± 0.08 | 2.15 ± 0.04 | 3.07 ± 0.02 | 0.28 ± 0.00 | 1.69 ± 0.01 | 14.50 | |
Bio-fertilizer | 0.93 ± 0.01 | 3.06 ± 0.04 | 2.35 ± 0.05 | 2.98 ± 0.04 | 3.82 ± 0.03 | 3.97 ± 0.07 | 0.84 ± 0.00 | 1.78 ± 0.01 | 19.73 | ||
Farmyard manure (FYM) | 100% | Control | 1.60 ± 0.05 | 1.51 ± 0.02 | 1.98 ± 0.04 | 3.15 ± 0.09 | 2.67 ± 0.02 | 3.56 ± 0.01 | 0.14 ± 0.00 | 1.89 ± 0.00 | 16.50 |
Bio-fertilizer | 1.37 ± 0.04 | 1.16 ± 0.03 | 2.06 ± 0.06 | 3.51 ± 0.02 | 3.39 ± 0.05 | 3.30 ± 0.05 | 0.05 ± 0.00 | 2.34 ± 0.01 | 17.18 | ||
50% | Control | 1.04 ± 0.01 | 1.09 ± 0.02 | 1.86 ± 0.02 | 3.11 ± 0.03 | 2.39 ± 0.04 | 3.12 ± 0.04 | 0.02 ± 0.00 | 1.45 ± 0.00 | 14.08 | |
Bio-fertilizer | 1.53 ± 0.04 | 2.57 ± 0.01 | 1.98 ± 0.03 | 3.47 ± 0.04 | 3.23 ± 0.03 | 3.24 ± 0.07 | 0.28 ± 0.00 | 2.11 ± 0.01 | 18.41 | ||
Mean | 1.12 | 2.21 | 2.34 | 3.10 | 2.82 | 3.34 | 0.36 | 1.94 | 17.24 | ||
Organic sources | Control | 0.72 c | 1.32 b | 2.23 c | 2.39 d | 1.76 c | 1.71 d | 0.25 c | 1.49 c | 11.86 d | |
Catch crop | 1.22 b | 3.00 a | 2.82 a | 3.86 a | 3.36 a | 4.54 a | 0.44 b | 2.25 a | 21.49 a | ||
Straw | 1.16 ab | 2.96 a | 2.34 b | 2.84 c | 3.25 ab | 3.83 b | 0.61 a | 2.11 ab | 19.08 b | ||
FYM | 1.39 a | 1.58 bc | 1.97 d | 3.31 b | 2.92 b | 3.31 c | 0.12 d | 1.95 bc | 16.54 c | ||
Mineral fertilization | 100% NPK fertilization | 1.13 a | 2.26 a | 2.36 a | 3.09 a | 2.78 a | 3.06 a | 0.40 a | 2.05 a | 17.14 a | |
50% NPK fertilization | 1.05 b | 2.06 b | 2.30 a | 3.03 a | 2.74 a | 3.31 a | 0.29 b | 1.79 b | 16.56 b | ||
Bio-fertilizer (Bio-F) | Control | 1.09 b | 1.78 b | 2.25 b | 3.00 b | 2.51 b | 3.24 b | 0.18 b | 1.93 b | 15.98 b | |
Bio-fertilizer | 1.16 a | 2.65 a | 2.43 a | 3.20 a | 3.13 a | 3.44 a | 0.53 a | 1.97 a | 18.51 a |
Organic Fertilization | Mineral NPK Fertilization | Bio-Fertilizer | Aspartic Acid | Asparagine | Alanine | Glutamic Acid | Sum |
---|---|---|---|---|---|---|---|
Control (without organic fertilizer) | 100% | Control | 6.37 ± 0.05 | 4.92 ± 0.04 | 0.22 ± 0.00 | 4.99 ± 0.02 | 16.50 |
Bio-fertilizer | 7.68 ± 0.07 | 5.83 ± 0.05 | 1.61 ± 0.04 | 6.72 ± 0.04 | 21.84 | ||
50% | Control | 4.47 ± 0.04 | 3.91 ± 0.02 | 0.22 ± 0.01 | 4.29 ± 0.05 | 12.89 | |
Bio-fertilizer | 6.95 ± 0.08 | 7.07 ± 0.07 | 0.22 ± 0.00 | 5.95 ± 0.01 | 20.19 | ||
Catch crop (pea) | 100% | Control | 7.51 ± 0.11 | 4.56 ± 0.04 | 1.22 ± 0.01 | 7.56 ± 0.08 | 25.85 |
Bio-fertilizer | 8.95 ± 0.12 | 4.86 ± 0.05 | 1.24 ± 0.01 | 8.48 ± 0.11 | 28.53 | ||
50% | Control | 4.64 ± 0.04 | 2.24 ± 0.02 | 1.02 ± 0.01 | 7.10 ± 0.04 | 15.00 | |
Bio-fertilizer | 6.62 ± 0.08 | 4.05 ± 0.03 | 1.15 ± 0.02 | 8.14 ± 0.03 | 19.96 | ||
Straw | 100% | Control | 6.06 ± 0.05 | 4.21 ± 0.05 | 0.27 ± 0.00 | 5.01 ± 0.05 | 15.55 |
Bio-fertilizer | 10.35 ± 0.14 | 4.97 ± 0.01 | 2.48 ± 0.02 | 8.40 ± 0.06 | 26.20 | ||
50% | Control | 5.86 ± 0.12 | 4.20 ± 0.02 | 0.31 ± 0.01 | 4.80 ± 0.05 | 15.17 | |
Bio-fertilizer | 8.57 ± 0.09 | 1.76 ± 0.01 | 0.67 ± 0.01 | 5.81 ± 0.02 | 16.81 | ||
Farmyard manure (FYM) | 100% | Control | 4.71 ± 0.08 | 3.53 ± 0.02 | 0.22 ± 0.00 | 2.88 ± 0.01 | 11.34 |
Bio-fertilizer | 6.12 ± 0.11 | 3.37 ± 0.01 | 0.31 ± 0.00 | 5.31 ± 0.05 | 15.11 | ||
50% | Control | 4.12 ± 0.05 | 2.36 ± 0.04 | 0.21 ± 0.00 | 2.31 ± 0.04 | 9.00 | |
Bio-fertilizer | 8.28 ± 0.06 | 1.89 ± 0.02 | 0.58 ± 0.01 | 7.50 ± 0.05 | 18.25 | ||
Mean | 6.70 | 3.98 | 0.75 | 5.95 | 17.39 | ||
Organic sources | Control | 6.37 c | 5.43 a | 0.57 c | 5.49 c | 17.86 c | |
Catch crop | 6.93 b | 3.93 b | 1.16 a | 7.82 a | 19.84 a | ||
Straw | 7.71 a | 3.79 b | 0.93 b | 6.01 b | 18.43 b | ||
FYM | 5.81 d | 2.79 c | 0.33 d | 4.50 d | 13.43 d | ||
Mineral fertilization | 100% NPK fertilization | 7.12 a | 4.57 a | 0.87 a | 6.04 a | 20.12 a | |
50% NPK fertilization | 6.19 b | 3.43 b | 0.55 b | 5.74 a | 17.66 b | ||
Bio-fertilizer | Control | 5.47 b | 3.74 b | 0.46 b | 4.87 b | 14.54 b | |
Bio-fertilizer | 7.94 a | 4.23 a | 1.03 a | 7.08 a | 20.24 a |
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Wichrowska, D.; Szczepanek, M. Possibility of Limiting Mineral Fertilization in Potato Cultivation by Using Bio-fertilizer and Its Influence on Protein Content in Potato Tubers. Agriculture 2020, 10, 442. https://doi.org/10.3390/agriculture10100442
Wichrowska D, Szczepanek M. Possibility of Limiting Mineral Fertilization in Potato Cultivation by Using Bio-fertilizer and Its Influence on Protein Content in Potato Tubers. Agriculture. 2020; 10(10):442. https://doi.org/10.3390/agriculture10100442
Chicago/Turabian StyleWichrowska, Dorota, and Małgorzata Szczepanek. 2020. "Possibility of Limiting Mineral Fertilization in Potato Cultivation by Using Bio-fertilizer and Its Influence on Protein Content in Potato Tubers" Agriculture 10, no. 10: 442. https://doi.org/10.3390/agriculture10100442