The most effective way to reduce the worldwide burden of liver cancer is to prevent it from happening in the first place. The current treatment of liver cancer has significant side effects. Hence, there is a need to develop anti-liver cancer agents of plant origin, which are less toxic, more efficacious and cost-effective. The present study has been performed experimentally by in vitro to examine the anti-liver cancer activity of roots of Bauhinia tomentosa L (Fabaceae). The roots of B. tomentosa was tested for its anti-cancer activity against HUH-7 human liver cancer cell lines by MTT assay. The standard used in this assay was Camptothecin (CPT) at 25µG concentration. Plant extract was tested at 25μg/mL, 50 μg/mL,100μg/mL, 200 μg/mL and 400 μg/mL concentrations. The percent cell viability of standard drug was found to be 49.59% and plant extracts at 25 μg/mL, 50 μg/mL,100 μg/mL, 200 μg/mL and 400 μg/mL concentrations were found to be 93.82%, 86.21%, 74.48%, 63.04%, 45.71% respectively. The cell morphology was observed and recorded under a microscope. The results clearly indicated that B. tomentosa shows a dose-dependent activity and it was maximum at 400μg/mL concentration where it shows 45.71% of liver cancer cell viability and it was comparable to the standard drug where it shows 49.59% of viability.