A method for simultaneous determination of 15 pesticides in cereals by HPLC with UV and FL detection was developed. The pesticides were extracted by SFE, defatted with Extrelut^®+Sep-Pak^® C₁₈ and cleaned up with a Sep-Pak^® Florisil cartridge. Pesticides were eluted from the cartridge with mixtures of 15 and 50% ether-n-hexane and 50% acetone-n-hexane.
Extrelut^® 3 was directly connected to the exhaust port of SFE to charge the extract. The amount of lipid extracted by SFE was smaller than that in the case of solvent extraction with acetone or acetonitrile. The large amount of lipid in the case of corn was further decreased by gel permeation chromatography (GPC) after Extrelut^® 3. Separation of the Sep-Pak^® Florisil eluate into three fractions was effective in providing a clue to the kinds of pesticide contained in the sample.